MANSC4 (NM_001146221) Human Tagged ORF Clone

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SKU
RC228271
MANSC4 (Myc-DDK-tagged)-Human MANSC domain containing 4 (MANSC4)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MANSC4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC228271 representing NM_001146221
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATGTGGCAGAGGTAGCAGTGAACGTGATATTGCTCCTAAGCATGGGGTGGACATCAGACTCTCTCT
GCTCACCCACAATTTTTTACAGAGACTGCTGGATCCGTCGCTTCCCAGGTCTTCTAATCAATCTGGAGGA
GTCTCAGAAGCTGGGAGCCCAGTTCTTGAAGTATTATTCTGAAAGCACTGGCCAGAAGTGCAGTAGGAGC
TGCTGTCTTCGGAAGGATGTTTCCTGTAACCTGGCTGTCTTCTACCACAGTCCTATTCATGACAATATCA
ACTGCCTCCATGTTCACTGCCCAACACTGGAGAGCTGCATATTAGAGCCTGGAACCAGTGCCATTTTGTA
CAACATAACAGACGGTATAGATCCGGATTTGCTGGTTTTTGAACAATCTCCCACATATCTAAATACTCGT
TCTTCATCCAATAGATGGGACAGACTAAGGATTCTAAAAGCTATGAATTTAGATAAACAAACCACCACGA
TAAATGGTATGCTGCCATCCACAGAGGCTCCATCCTCAACCACGCATCAAGATTTGGTTGTAAACACAAA
CAGTACCAGTTATTCTAAGGAATTAACCACAGATTTTTGGGCAAGATTTACTTCCCTGAATGAGTCCATT
ACCACAAAGATAAATAAGGTGTCACCAAGTACTGATTTCATCAGCAATCCAGATAATAAGACTATTTCTC
CTTTCTTTGAACCCATAGACACAAAACTTTCTCATATGCCTGTTCCACCTGGACTCAACAGTAGCAAACA
ATTACTAAACAAAACCAAAGGATACAATAGCAGAAACCACACATCTGCAAATGAAGATGAGGTATCTGTG
ACTTCAAAGACTTGGCTGGTTTCTGTGGCCCTTTGCACCTCTGTCATCTTTCTCGGCTGTTGTATAGTCA
TCCTGGCATCTGGATGCTGTGGAAAGCAGCAGGGCCAGTATAAACCAGGACAGAGAAAATCAGGATCCTT
GCAAATTAAAAACCGTAACCATATGAAGGAGAACTCTTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC228271 representing NM_001146221
Red=Cloning site Green=Tags(s)

MHVAEVAVNVILLLSMGWTSDSLCSPTIFYRDCWIRRFPGLLINLEESQKLGAQFLKYYSESTGQKCSRS
CCLRKDVSCNLAVFYHSPIHDNINCLHVHCPTLESCILEPGTSAILYNITDGIDPDLLVFEQSPTYLNTR
SSSNRWDRLRILKAMNLDKQTTTINGMLPSTEAPSSTTHQDLVVNTNSTSYSKELTTDFWARFTSLNESI
TTKINKVSPSTDFISNPDNKTISPFFEPIDTKLSHMPVPPGLNSSKQLLNKTKGYNSRNHTSANEDEVSV
TSKTWLVSVALCTSVIFLGCCIVILASGCCGKQQGQYKPGQRKSGSLQIKNRNHMKENSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001146221
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001146221.5
RefSeq ORF 1023 bp
Locus ID 100287284
UniProt ID A6NHS7
Cytogenetics 12p11.22
MW 37.7 kDa
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Documents
Resources
"NM_001146221" in other vectors (4)
SKU Description Size Price
RC228271L3 Lenti ORF clone of Human MANSC domain containing 4 (MANSC4), Myc-DDK-tagged 10 ug
$757.00
RC228271L4 Lenti ORF clone of Human MANSC domain containing 4 (MANSC4), mGFP tagged 10 ug
$757.00
RG228271 MANSC4 (tGFP-tagged) - Human MANSC domain containing 4 (MANSC4) 10 ug
$657.00
SC326906 MANSC4 (untagged)-Human MANSC domain-containing protein (LOC100133893) 10 ug
$503.00

Plasmid Map for RC228271

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Cloning scheme for RC228271

Circular map for RC228271

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