CBY3 (NM_001164444) Human Tagged ORF Clone

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SKU
RC228176
CBY3 (Myc-DDK-tagged)-Human chibby homolog 3 (Drosophila) (CBY3)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CBY3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC228176 representing NM_001164444
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGCTTCCAGAGACCATCTCCCTGAACCAGATCTTGGGGATGCAGCGCCCCCTGGCTCGCCTTCAT
CATTCTGGACATCCGGGCTCCCGAGACAAGAGCGCAGCACGAGTCGCCAGCGCTCCCGAGGCTCCCCTTC
GAGCACCTGCGTGCCCTACAAGGTCCACGCCCTGGCAACCTTCGAGTGCTCGGCTACGAGCCATGCCAGC
CGCCTGTGGCAGACGCTGCAGCAGTTTTGGGCCGATCACATCTCGCGGCCCTTCTCGCCACGGCGGCCGC
CACTGCGCCGCATGCCCTCCCTGTCCACCTTCTACCTGCTGGACCACAACACGCGCCAGGCCGAGCTGGG
CCTCGCCTACGGCGCGCCGTGCATGCGCCTCAGCAACCAGGCCTTCGTGTTCCGCGGCGGGCGGTGGACC
ACTGAGAGCCAGCTGGCGAGGACGCGGTCGCCGCTGCTCTCGCGGACCGCCTGGGGCTGGAAGGCGCAGG
TGCAGCGGTCCAAAAGCCAAGTGTTGCTGGAGGAGAACAACTACCTGAAGCTGCAGCAGGAACTGCTCAT
AGACATGCTGACTGAGACCATGGCGCGCATGCACTTGCTGGAGAAGCAGCGCAACCCCGAGGTGATCCCG
ACGGCTGCGGCGCGCGCCGGGCAGAGGAAGATGCGCAAGCGCGCAGGCGCCAGCGCGGGCGTGCTCATGA
TCCAGCCGTGCGCTCTGGACTCGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC228176 representing NM_001164444
Red=Cloning site Green=Tags(s)

MWASRDHLPEPDLGDAAPPGSPSSFWTSGLPRQERSTSRQRSRGSPSSTCVPYKVHALATFECSATSHAS
RLWQTLQQFWADHISRPFSPRRPPLRRMPSLSTFYLLDHNTRQAELGLAYGAPCMRLSNQAFVFRGGRWT
TESQLARTRSPLLSRTAWGWKAQVQRSKSQVLLEENNYLKLQQELLIDMLTETMARMHLLEKQRNPEVIP
TAAARAGQRKMRKRAGASAGVLMIQPCALDSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164444
ORF Size 726 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164444.2
RefSeq ORF 729 bp
Locus ID 646019
UniProt ID A6NI87
Cytogenetics 5q35.3
MW 27.2 kDa
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Documents
Resources
"NM_001164444" in other vectors (4)
SKU Description Size Price
RC228176L3 Lenti ORF clone of Human chibby homolog 3 (Drosophila) (CBY3), Myc-DDK-tagged 10 ug
$600.00
RC228176L4 Lenti ORF clone of Human chibby homolog 3 (Drosophila) (CBY3), mGFP tagged 10 ug
$600.00
RG228176 CBY3 (tGFP-tagged) - Human chibby homolog 3 (Drosophila) (CBY3) 10 ug
$500.00
SC326811 CBY3 (untagged)-Human chibby homolog 3 (Drosophila) (CBY3) 10 ug
$330.00

Plasmid Map for RC228176

Download sequence for

Cloning scheme for RC228176

Circular map for RC228176

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