LFA3 (CD58) (NM_001144822) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LFA3 |
| Synonyms | ag3; LFA-3; LFA3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001144822 |
| ORF Size | 744 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001144822.1, NP_001138294.1 |
| RefSeq ORF | 747 bp |
| Locus ID | 965 |
| UniProt ID | P19256 |
| Cytogenetics | 1p13.1 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| MW | 27.8 kDa |
| Summary | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. provided by RefSeq, Jan 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001144822" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC227634L1 | Lenti ORF clone of Human CD58 molecule (CD58), transcript variant 2, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC227634L2 | Lenti ORF clone of Human CD58 molecule (CD58), transcript variant 2, mGFP tagged | 10 ug |
$750.00
|
|
| RC227634L3 | Lenti ORF clone of Human CD58 molecule (CD58), transcript variant 2, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC227634L4 | Lenti ORF clone of Human CD58 molecule (CD58), transcript variant 2, mGFP tagged | 10 ug |
$750.00
|
|
| RG227634 | CD58 (tGFP-tagged) - Human CD58 molecule (CD58), transcript variant 2 | 10 ug |
$650.00
|
|
| SC325661 | CD58 (untagged)-Human CD58 molecule (CD58), transcript variant 2 | 10 ug |
$330.00
|
|
Citations
Cognate T-cell labeling by conventional and unconventional antigen-presenting cells in vivo using a modular SrtA-based toolkit
Samson, Farsakoglu, Lenaerts et al
bioRxiv (2026)
Samson, Farsakoglu, Lenaerts et al
bioRxiv (2026)
AI Summary: OriGene’s CD58 (NM_001144822) Human Tagged ORF Clone Lentiviral Particle (RC227634) was used as a template to generate the flag-SrtA-hCD58 fragment, which was essential for constructing the modular LIPSTIC toolkit described in the study.
Snippet: For the pMY-flag-SrtA-hCD58-IRES-GFP construct, an intermediate flag-SrtA-hCD58 fragment was amplified by PCR from the pMY-flag-SrtA-CD40-IRES-GFP and the CD58 (NM_001144822) Human Tagged ORF Clone Lentiviral Particle (RC227634, Origene). Next, the fragment was inserted into XhoI and NotI sites of a pMY-IRES-GFP plasmid
Showing 1-1 of 1 papers.
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in shipping.
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