SHISA9 (NM_001145204) Human Tagged ORF Clone

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SKU
RC227529
SHISA9 (Myc-DDK-tagged)-Human shisa homolog 9 (Xenopus laevis) (SHISA9), transcript variant 1
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SHISA9
Synonyms CKAMP44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227529 representing NM_001145204
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCGCGTCCTTCGGCTGCTCCTCGGTTGCTTCCTCACCGAGCTGTGCGCCCGCGTGTGCCGGGCGC
AGGAGCGAGCGGGACACGGGCAGCTGGCGCAACTGGGCGGCGTGTTGCTGCTGGCGGGGGGCAACCGCTC
CGGGGCCGCCTCCGGAGAGGCCAGCGAGGGCGCTGAGGCATCGGACGCGCCCCCGACCCGGGCGCCCACG
CCGGACTTCTGCCGGGGCTACTTCGATGTCATGGGCCAGTGGGACCCGCCGTTCAACTGCAGCTCGGGCG
ACTTCATCTTCTGCTGCGGGACTTGTGGCTTCCGGTTCTGCTGCACGTTTAAGAAGCGGCGACTGAACCA
AAGCACCTGCACCAACTACGACACGCCGCTCTGGCTCAACACCGGCAAGCCCCCCGCCCGCAAGGACGAC
CCCTTGCACGACCCCACCAAGGACAAGACCAACCTGATCGTCTACATCATCTGCGGGGTGGTGGCCGTCA
TGGTGCTCGTGGGCATCTTCACCAAGCTGGGGCTGGAGAAAGCGCACCGGCCCCAAAGGGAGCACATGTC
CAGGGCCCTTGCGGATGTCATGAGACCACAGGGCCACTGCAACACTGATCACATGGAGAGAGACCTAAAC
ATCGTTGTCCACGTCCAGCATTATGAGAACATGGACACGAGAACCCCCATAAATAATCTTCATGCCACCC
AGATGAACAACGCAGTGCCCACCTCTCCTCTGCTCCAGCAGATGGGCCATCCACATTCGTACCCGAACCT
GGGCCAGATCTCCAACCCCTATGAACAGCAGCCACCAGGAAAAGAGCTCAACAAGTACGCCTCCTTAAAG
GCAGTCGGAAGTTCTGATGGTGACTGGGCAGTATCGACACTTAAGTCACCAAAAGCTGACAAGGTCAATG
ACGACTTCTACACCAAGCGACGGCACCTGGCTGAGCTGGCTGCCAAGGGGAACTTACCTCTGCACCCCGT
AAGAGTGGAGGACGAGCCCCGGGCCTTCAGCCCTGAGCACGGTCCTGCCAAGCAGAATGGACAGAAGTCC
CGCACCAACAAGATGCCCCCACATCCCCTGGCCTACACCTCTACCACCAACTTTAAGGGCTGGGACCCCA
ACGAGCAGTCCCTCCGGCGGCAGGCTTACAGCAACAAGGGCAAGCTTGGCACGGCCGAGACAGGCTCCAG
CGACCCCTTGGGAACTCGCCCCCAGCACTACCCACCCCCACAGCCATACTTCATCACCAACAGCAAAACA
GAAGTGACTGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC227529 representing NM_001145204
Red=Cloning site Green=Tags(s)

MRRVLRLLLGCFLTELCARVCRAQERAGHGQLAQLGGVLLLAGGNRSGAASGEASEGAEASDAPPTRAPT
PDFCRGYFDVMGQWDPPFNCSSGDFIFCCGTCGFRFCCTFKKRRLNQSTCTNYDTPLWLNTGKPPARKDD
PLHDPTKDKTNLIVYIICGVVAVMVLVGIFTKLGLEKAHRPQREHMSRALADVMRPQGHCNTDHMERDLN
IVVHVQHYENMDTRTPINNLHATQMNNAVPTSPLLQQMGHPHSYPNLGQISNPYEQQPPGKELNKYASLK
AVGSSDGDWAVSTLKSPKADKVNDDFYTKRRHLAELAAKGNLPLHPVRVEDEPRAFSPEHGPAKQNGQKS
RTNKMPPHPLAYTSTTNFKGWDPNEQSLRRQAYSNKGKLGTAETGSSDPLGTRPQHYPPPQPYFITNSKT
EVTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001145204
ORF Size 1272 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145204.3
RefSeq ORF 1275 bp
Locus ID 729993
UniProt ID B4DS77
Cytogenetics 16p13.12
MW 46.7 kDa
Summary Regulator of short-term neuronal synaptic plasticity in the dentate gyrus. Associates with AMPA receptors (ionotropic glutamate receptors) in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001145204" in other vectors (4)
SKU Description Size Price
RC227529L3 Lenti ORF clone of Human shisa homolog 9 (Xenopus laevis) (SHISA9), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC227529L4 Lenti ORF clone of Human shisa homolog 9 (Xenopus laevis) (SHISA9), transcript variant 1, mGFP tagged 10 ug
$986.00
RG227529 SHISA9 (tGFP-tagged) - Human shisa homolog 9 (Xenopus laevis) (SHISA9), transcript variant 1 10 ug
$886.00
SC325965 SHISA9 (untagged)-Human shisa homolog 9 (Xenopus laevis) (SHISA9), transcript variant 1 10 ug
$686.00

Plasmid Map for RC227529

Download sequence for

Cloning scheme for RC227529

Circular map for RC227529

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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