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CCDC42B (CFAP73) (NM_001144872) Human Tagged ORF Clone

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SKU
RC227433
CCDC42B (Myc-DDK-tagged)-Human coiled-coil domain containing 42B (CCDC42B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC42B
Synonyms CCDC42B; MIA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227433 representing NM_001144872
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGCCCTGGGAGGAATATTTCCGACTGGCTTTGCAAGAGAAACTGTCTACAAAGCTGCCAGAGC
AGGCTGAGGATCATGTCCCACCAGTACTGCGTCTCCTGGAGAAGAGGCAAGAGCTGGTAGATGCAGACCA
GGCCCTGCAGGCCCAGAAGGAGGTGTTCCGCACCAAGACGGCAGCCCTGAAACAGCGTTGGGAACAGCTG
GAACAAAAGGAGCGGGAGCTAAAGGGATCGTTCATCCGCTTTGACAAGTTTTTGCAGGACTCCGAGGCCC
GGCGCAATCGCGCGCTGCGGAGGGCGGCGGAGGAGAGGCACCAGGCGGGCCGTCGGGAGGTGGAGGCGCT
GCGTCTGTGGACCCAGCTCCAGGAGCTACGGCGGGAACACGCGCGGCTGCAGCGCCGGCTTAAGCGCCTG
GAGCCCTGCGCGCGCCTGCTGGAGCAAGCGCTGGAGCTGCTGCCCGGGTTCCAAGAGGTTCCGGAGCTGG
TGGCGCGCTTCGACGGCCTGGCCGAGACGCAGGCGGCGCTGAGGCTCAGGGAGCGCGAGCAGCTCGCGGA
GCTGGAGGCGGCGCGAGCGCGGCTGCAGCAGCTGCGGGACGCCTGGCCGGACGAGGTGCTCGCACAGGGC
CAGCGGCGGGCACAGCTGCAGGAGCGCCTGGAGGCTGCCAGGGAGCGTACGCTGCAGTGGGAATCCAAGT
GGATTCAGATTCAGAACACAGCAGCGGAGAAGACTCTGCTCCTGGGACGCAGCAGGATGGCTGTGCTCAA
CCTGTTCCAGCTAGTGTGCCAGCATCAGGGGCAGCCTCCCACCCTGGACATCGAGGACACGGAGGGACAG
CTAGAGCACGTGAAGCTGTTCATGCAGGACCTCTCTGCCATGCTGGCCGGCCTGGGTCAGGCTGAGCCTG
CAGCCCCTGCCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227433 representing NM_001144872
Red=Cloning site Green=Tags(s)

MAVPWEEYFRLALQEKLSTKLPEQAEDHVPPVLRLLEKRQELVDADQALQAQKEVFRTKTAALKQRWEQL
EQKERELKGSFIRFDKFLQDSEARRNRALRRAAEERHQAGRREVEALRLWTQLQELRREHARLQRRLKRL
EPCARLLEQALELLPGFQEVPELVARFDGLAETQAALRLREREQLAELEAARARLQQLRDAWPDEVLAQG
QRRAQLQERLEAARERTLQWESKWIQIQNTAAEKTLLLGRSRMAVLNLFQLVCQHQGQPPTLDIEDTEGQ
LEHVKLFMQDLSAMLAGLGQAEPAAPAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001144872
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001144872.2
RefSeq ORF 927 bp
Locus ID 387885
UniProt ID A6NFT4
Cytogenetics 12q24.13
MW 35.7 kDa
Summary May play a role in ciliary/flagellar motility by regulating the assembly and the activity of axonemal inner dynein arm.UniProtKB/Swiss-Prot Function
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"NM_001144872" in other vectors (4)
SKU Description Size Price
RC227433L3 Lenti ORF clone of Human coiled-coil domain containing 42B (CCDC42B), Myc-DDK-tagged 10 ug
$600.00
RC227433L4 Lenti ORF clone of Human coiled-coil domain containing 42B (CCDC42B), mGFP tagged 10 ug
$600.00
RG227433 CCDC42B (tGFP-tagged) - Human coiled-coil domain containing 42B (CCDC42B) 10 ug
$500.00
SC325750 CCDC42B (untagged)-Human coiled-coil domain containing 42B (CCDC42B) 10 ug
$330.00

Plasmid Map for RC227433

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227433
Circular map for RC227433

file-alt Citations

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