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SH2D4B (NM_001145719) Human Tagged ORF Clone

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SKU
RC227417
SH2D4B (Myc-DDK-tagged)-Human SH2 domain containing 4B (SH2D4B), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SH2D4B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227417 representing NM_001145719
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCCAGAGGACTCAGGAGGCTCTCCAGATGCTGCAGCGAGTGACAAGCACATCCAATGGCTCCTAG
GGGCAGATGGCGAGGTCTGGGTCTGGATCATGGGAGAAGGCCCTGGTGACAAGCCCTACGAAGAGATCTC
TGAGGAGCTGATTGCAGAGAGGGCGCGGCTGCAGGCACAGAGGGAAGCTGAGGAGCTCTGGAGACAGAAG
GAGGCAGAGATCACCAAGAAGTTCCGGGATGCTCTGGCCAATGAGAAAGCCCGGATCTTGGCGGAGAAGT
GGAAAGTGGAGATGGAAGACCGCAAGGCTGCCAAAGTCCTGGAGGAACGCATCCACGAGGAATTCAAGAG
GAAAGAGGAAGAGGAGAGGAAGCGAGGAGAAGAGCAGATTCGCCTCCAGGAAGAGCAGAGGGCGAAGGAG
CTCTACTGGACCCTGAAGCAGGCTCAGCTGCATTGCCAAGCCAGTGAGAAAGAGGAGCGAGAGTGGGAAG
AACAGTTGCGCCGGTCCAAGGCGGCTGATGAGGAGAGGAGCCGCCGAGCCCAGCGCGCCCGGGACGAGTA
CCGACACCACTCGCTCCGTGCTATCCAGAAGGGCACGGTCGCTGGCCTCAGCTCCATGTTCCGGGAGCTT
GGCCAGAGCCATGAGCAGGAGGCAAGACTCTACCACCACCTCCCCGACCCGGGTCTGCCGCAGCCCCTTG
CCCTGCCGGTCAGCAGGACCTGGGAGCGCCCGCTGCGCCCAGTCTCCAGAGATGTCATCGTCCGCTGGTT
TAAGGAGGAGCAGCTGCCTCGCCGAGCTGGCTTCGAGAGGAACACCAAGTTCATCGCCCCCTGGTTCCAT
GGAGGAAATTATCACTGTTTCAGGAGGAGAGTTACTTCAGGAACCCTGCGGACAGAGGGACAGCCCACCA
GACTACCATCTGTTGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227417 representing NM_001145719
Red=Cloning site Green=Tags(s)

MLPEDSGGSPDAAASDKHIQWLLGADGEVWVWIMGEGPGDKPYEEISEELIAERARLQAQREAEELWRQK
EAEITKKFRDALANEKARILAEKWKVEMEDRKAAKVLEERIHEEFKRKEEEERKRGEEQIRLQEEQRAKE
LYWTLKQAQLHCQASEKEEREWEEQLRRSKAADEERSRRAQRARDEYRHHSLRAIQKGTVAGLSSMFREL
GQSHEQEARLYHHLPDPGLPQPLALPVSRTWERPLRPVSRDVIVRWFKEEQLPRRAGFERNTKFIAPWFH
GGNYHCFRRRVTSGTLRTEGQPTRLPSVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001145719
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145719.1, NP_001139191.1
RefSeq ORF 930 bp
Locus ID 387694
UniProt ID Q5SQS7
Cytogenetics 10q23.1
MW 36.3 kDa
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Documents
Resources
"NM_001145719" in other vectors (4)
SKU Description Size Price
RC227417L3 Lenti ORF clone of Human SH2 domain containing 4B (SH2D4B), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC227417L4 Lenti ORF clone of Human SH2 domain containing 4B (SH2D4B), transcript variant 2, mGFP tagged 10 ug
$600.00
RG227417 SH2D4B (tGFP-tagged) - Human SH2 domain containing 4B (SH2D4B), transcript variant 2 10 ug
$500.00
SC326556 SH2D4B (untagged)-Human SH2 domain containing 4B (SH2D4B), transcript variant 2, mRNA 10 ug
$330.00

Plasmid Map for RC227417

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227417
Circular map for RC227417

file-alt Citations

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