OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

TRAF4AF1 (KNSTRN) (NM_001142761) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC227397
KNSTRN (Myc-DDK-tagged)-Human chromosome 15 open reading frame 23 (C15orf23), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA333584 Rabbit Polyclonal Anti-KNSTRN Antibody
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "TRAF4AF1 or NM_001142761"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRAF4AF1
Synonyms C15orf23; HSD11; SKAP; TRAF4AF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227397 representing NM_001142761
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTCCCGAAGCCCCGCCCCTGGACAGAGTTTTCCGTACAACATGGCTGTCTACAGAGTGCGATT
CCCACCCACTTCCGCCTAGCTACCGGAAGTTTCTATTTGAAACCCAGGCGGCCGACTTAGCCGGTGGCAC
GACAGTTGCTGCAGGGAATCTTTTAAACGAGAGCGAGAAGGACTGCGGGCAGGACCGGCGGGCTCCTGGG
GTTCAGCCGTGCCGCCTCGTTACGATGACCAGTGTGGTTAAGACAGTGTATAGCCTGCAGCCCCCCTCTG
CGCTGAGCGGCGGCCAGCCGGCAGACACACAAACTCGGGCCACTTCTAAGAGTCTCTTACCTGTTAGGTC
CAAAGAAGTCGATGTTTCCAAACAGCTTCATTCAGGAGGTCCAGAGAATGATGTTACAAAAATCACCAAA
CTGAGACGAGAGAATGGGCAAATGAAAGCTACTGACACTGCCACCAGAAGGAATGTCAGAAAAGGCTACA
AACCACTGAGTAAGCAAAAATCAGAGGAAGAGCTCAAGGACAAGAACCAGCTGTTAGAAGCCGTCAACAA
GCAGTTGCACCAGAAGTTGACTGAAACTCAGGGAGAGCTGAAGGACCTGACCCAGAAGGTAGAGCTGCTG
GAGAAGTTTCGGGACAACTGTTTGGCAATTTTGGAGAGCAAGGGCCTTGATCCAGCTTTAGGCAGTGAGA
CCCTGGCATCACGACAAGAATCCACTACTGATCACATGGACTCTATGTTGCTGTTAGAAACTTTGCAAGA
GGAGCTGAAGCTTTTTAACGAAACAGCCAAAAAGCAGATGGAGGAGTTACAGATTGCTTGGATGAATCAT
GGGATATTGCACCAAATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227397 representing NM_001142761
Red=Cloning site Green=Tags(s)

MAAPEAPPLDRVFRTTWLSTECDSHPLPPSYRKFLFETQAADLAGGTTVAAGNLLNESEKDCGQDRRAPG
VQPCRLVTMTSVVKTVYSLQPPSALSGGQPADTQTRATSKSLLPVRSKEVDVSKQLHSGGPENDVTKITK
LRRENGQMKATDTATRRNVRKGYKPLSKQKSEEELKDKNQLLEAVNKQLHQKLTETQGELKDLTQKVELL
EKFRDNCLAILESKGLDPALGSETLASRQESTTDHMDSMLLLETLQEELKLFNETAKKQMEELQIAWMNH
GILHQM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001142761
ORF Size 858 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001142761.1, NP_001136233.1
RefSeq ORF 861 bp
Locus ID 90417
UniProt ID Q9Y448
Cytogenetics 15q15.1
MW 31.7 kDa
Summary Essential component of the mitotic spindle required for faithful chromosome segregation and progression into anaphase (PubMed:19667759). Promotes the metaphase-to-anaphase transition and is required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:19667759, PubMed:22110139). The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments (PubMed:21402792). Required for kinetochore oscillations and dynamics of microtubule plus-ends during live cell mitosis, possibly by forming a link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division (PubMed:23035123). May be involved in UV-induced apoptosis via its interaction with PRPF19; however, these results need additional evidences (PubMed:24718257).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001142761" in other vectors (4)
SKU Description Size Price
RC227397L3 Lenti ORF clone of Human chromosome 15 open reading frame 23 (C15orf23), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC227397L4 Lenti ORF clone of Human chromosome 15 open reading frame 23 (C15orf23), transcript variant 2, mGFP tagged 10 ug
$600.00
RG227397 KNSTRN (tGFP-tagged) - Human chromosome 15 open reading frame 23 (C15orf23), transcript variant 2 10 ug
$500.00
SC325712 KNSTRN (untagged)-Human chromosome 15 open reading frame 23 (C15orf23), transcript variant 2 10 ug
$330.00

Plasmid Map for RC227397

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227397
Circular map for RC227397

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products