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PLET1 (NM_001145024) Human Tagged ORF Clone

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SKU
RC227346
C11orf34 (Myc-DDK-tagged)-Human chromosome 11 open reading frame 34 (C11orf34)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLET1
Synonyms C11orf34
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227346 representing NM_001145024
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGTCTTCCATGACATGCTGCTGCAGCCACTGGGGATGTTTCTGTGCCTCAGTCTGCAGCTTTCTT
CTGCCACCTTTATAAGGTACAGTAGCACCTGCTTCACCTTTGATGAATACTACACCATAACCCTAGACAT
CAAGGCCAGTTCACATATCTACGAAAGCAATGCAGTCTATTCTGTATTTGTTCCCGTGAATGACAGCGTC
TATGCTGTGGTCATGAAAACCTTGGACGAGAACAGTGACTCAGCGGGCCTCTGGCAAAGAGCGGATAAAA
ATTGCTACAGCAACTCCACGTATTACGTGAAAGATCAATACATGACGGTCTTAGAGGCACAGTGGCAAGC
TCCTGAACCTGAGAACATAACTGAAGTGGAGATACAAGCTTTCACTGTCCAGATCAGAGCGCTGCCTATA
CTTTCTACTCTGAAGCTAAGAGAAAAACTGTCAACCTTAGCCTTAGCTGCCAAGATTCCCCAGAGCTCAG
CCTTCAAGCCTTTCTTCATGATTACACCCAAGAGTATCAGACTCGAAGGCTTGGCCAACCAAGTCTTCAG
CAGCCCCATCACAGAGGCCATTTATATCCTGCTTGCTTTTCTCACCAGCACACTTCTCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227346 representing NM_001145024
Red=Cloning site Green=Tags(s)

MAVFHDMLLQPLGMFLCLSLQLSSATFIRYSSTCFTFDEYYTITLDIKASSHIYESNAVYSVFVPVNDSV
YAVVMKTLDENSDSAGLWQRADKNCYSNSTYYVKDQYMTVLEAQWQAPEPENITEVEIQAFTVQIRALPI
LSTLKLREKLSTLALAAKIPQSSAFKPFFMITPKSIRLEGLANQVFSSPITEAIYILLAFLTSTLLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001145024
ORF Size 621 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145024.1, NP_001138496.1
RefSeq ORF 624 bp
Locus ID 349633
UniProt ID Q6UQ28
Cytogenetics 11q23.1
MW 23.2 kDa
Summary Modulates leading keratinocyte migration and cellular adhesion to matrix proteins during a wound-healing response and promotes wound repair. May play a role during trichilemmal differentiation of the hair follicle (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001145024" in other vectors (6)
SKU Description Size Price
RC227346L1 Lenti ORF clone of Human chromosome 11 open reading frame 34 (C11orf34), Myc-DDK-tagged 10 ug
$600.00
RC227346L2 Lenti ORF clone of Human chromosome 11 open reading frame 34 (C11orf34), mGFP tagged 10 ug
$600.00
RC227346L3 Lenti ORF clone of Human chromosome 11 open reading frame 34 (C11orf34), Myc-DDK-tagged 10 ug
$600.00
RC227346L4 Lenti ORF clone of Human chromosome 11 open reading frame 34 (C11orf34), mGFP tagged 10 ug
$600.00
RG227346 C11orf34 (tGFP-tagged) - Human chromosome 11 open reading frame 34 (C11orf34) 10 ug
$500.00
SC325605 C11orf34 (untagged)-Human chromosome 11 open reading frame 34 (C11orf34) 10 ug
$330.00

Plasmid Map for RC227346

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227346
Circular map for RC227346

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