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CBWD1 (NM_001145356) Human Tagged ORF Clone

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SKU
RC227294
CBWD1 (Myc-DDK-tagged)-Human COBW domain containing 1 (CBWD1), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CBWD1
Synonyms COBP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227294 representing NM_001145356
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTACCGGCTGTTGGATCTGCGGATGAGGAGGAGGATCCTGCGGAGGAGGATTGTCCTGAATTGGTTC
CCATTGAGACGACGCAAAGCGAGGAGGAGGAAAAGTCTGGCCTCGGCGCCAAGATCCCAGTCACAATTAT
CACCGGGTATTTAGGTGCTGGGAAGACAACACTTCTGAACTATATTTTGACAGAGCAACATAGTAAAAGA
GTAGCGGTCATTTTAAATGAATTTGGGGAAGGAAGTGCGCTGGAGAAATCCTTAGCTGTCAGCCAAGGTG
GAGAGCTCTATGAAGAGTGGCTGGAACTTAGAAACGGTTGCCTCTGCTGTTCAGTGAAGGACAGTGGCCT
TAGAGCTATTGAGAATTTGATGCAAAAGAAGGGGAAATTTGATTACATACTGTTAGAGACCACTGGATTA
GCAGACCCTGGTGCAGTGGCTTCTATGTTTTGGGTTGATGCTGAATTAGGGAGTGATATTTATCTTGATG
GTATCATAACTATTGTGGATTCAAAATATGGATTAAAACATTTAACAGAAGAGAAACCTGATGGCCTTAT
CAATGAAGCTACTAGACAAGTTGCTTTGGCAGATGCCATTCTCATTAATAAAACAGACCTGGTTCCAGAA
GAAGATGTAAAGAAATTAAGAACGACAATTAGATCCATAAATGGACTAGGACAAATCTTAGAAACACAAA
GATCAAGTTTGCAGAAAAAACTTCAGCATGTGCCAGGAACACAACCTCACCTTGATCAGAGTATTGTTAC
AATCACATTTGAAGTACCAGGAAATGCAAAGGAAGAACATCTTAATATGTTTATTCAGAATCTCCTGTGG
GAAAAGAATGTGAGAAACAAGGACAATCACTGCATGGAGGTCATAAGGCTGAAGGGATTGGTGTCAATCA
AAGACAAATCACAACAAGTGATTGTCCAGGGTGTCCATGAGCTCTATGATCTGGAGGAGACTCCAGTGAG
CTGGAAGGATGACACTGAGAGAACAAATCGATTGGTCCTCCTTGGCAGAAATTTAGATAAGGATATCCTT
AAACAGCTGTTTATAGCTACTGTGACAGAAACAGAAAAGCAGTGGACAACACGTTTCCAAGAAGATCAAG
TTTGTACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227294 representing NM_001145356
Red=Cloning site Green=Tags(s)

MLPAVGSADEEEDPAEEDCPELVPIETTQSEEEEKSGLGAKIPVTIITGYLGAGKTTLLNYILTEQHSKR
VAVILNEFGEGSALEKSLAVSQGGELYEEWLELRNGCLCCSVKDSGLRAIENLMQKKGKFDYILLETTGL
ADPGAVASMFWVDAELGSDIYLDGIITIVDSKYGLKHLTEEKPDGLINEATRQVALADAILINKTDLVPE
EDVKKLRTTIRSINGLGQILETQRSSLQKKLQHVPGTQPHLDQSIVTITFEVPGNAKEEHLNMFIQNLLW
EKNVRNKDNHCMEVIRLKGLVSIKDKSQQVIVQGVHELYDLEETPVSWKDDTERTNRLVLLGRNLDKDIL
KQLFIATVTETEKQWTTRFQEDQVCT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001145356
ORF Size 1128 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145356.1, NP_001138828.1
RefSeq ORF 1131 bp
Locus ID 55871
UniProt ID Q9BRT8
Cytogenetics 9p24.3
MW 41.8 kDa
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"NM_001145356" in other vectors (4)
SKU Description Size Price
RC227294L3 Lenti ORF clone of Human COBW domain containing 1 (CBWD1), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC227294L4 Lenti ORF clone of Human COBW domain containing 1 (CBWD1), transcript variant 3, mGFP tagged 10 ug
$757.00
RG227294 CBWD1 (tGFP-tagged) - Human COBW domain containing 1 (CBWD1), transcript variant 3 10 ug
$657.00
SC325842 CBWD1 (untagged)-Human COBW domain containing 1 (CBWD1), transcript variant 3 10 ug
$503.00

Plasmid Map for RC227294

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227294
Circular map for RC227294

file-alt Citations

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