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Invadolysin (LMLN) (NM_001136049) Human Tagged ORF Clone

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SKU
RC227243
LMLN (Myc-DDK-tagged)-Human leishmanolysin-like (metallopeptidase M8 family) (LMLN), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $634.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Invadolysin
Synonyms GP63; INV; IX14; LMNL1; MSP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227243 representing NM_001136049
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAACGACGCTCGGCCCGAAGATGGCGGCCGAATGGGGCGGAGGAGTGGGTTACTCGGGCTCAGGCC
CGGGCCGGAGCCGGTGGCGCTGGAGCGGGTCTGTGTGGGTCCGAAGCGTTTTACTCCTGTTGGGCGGGCT
CCGGGCCAGCGCCACATCTACTCCCGTCTCCTTGGGCAGTTCCCCTCCCTGCCGGCACCACGTCCCCTCT
GACACTGAGGTCATAAATAAAGTTCATCTTAAGGCAAATCATGTGGTCAAGAGAGATGTTGATGAGCATT
TAAGAATCAAGACTGTCTATGATAAAAGTGTTGAAGAGTTGCTCCCTGAGAAAAAGAATCTTGTAAAGAA
CAAGCTTTTCCCACAAGCGATTTCTTATTTAGAGAAGACTTTTCAGGTCCGTCGACCTGCGGGCACTATC
TTACTTAGCAGACAATGTGCAACAAACCAATACCTCCGGAAGGAAAACGATCCTCACAGGTACTGCACCG
GGGAGTGTGCCGCACACACAAAGTGCGGCCCCGTTATTGTTCCTGAGGAACATCTCCAGCAATGCCGGGT
CTACCGTGGGGGTAAGTGGCCTCATGGAGCAGTGGGTGTGCCAGACCAAGAAGGCATCTCAGATGCAGAC
TTTGTTCTTTACGTTGGTGCTCTGGCCACCGAGAGATGCAGCCATGAAAACATCATCTCTTATGCAGCCT
ATTGTCAGCAGGAAGCAAACATGGACAGGCCAATAGCAGGATATGCTAACCTGTGTCCAAATATGATCTC
TACCCAGCCTCAGGAGTTTGTTGGGATGCTGTCCACAGTGAAACATGAGGTTATTCATGCCCTGGGTTTC
TCTGCTGGGCTGTTTGCATTCTACCATGATAAAGATGGAAATCCTCTCACTTCAAGATTTGCAGATGGCC
TCCCACCTTTTAATTATAGTCTGGGATTATATCAATGGAGTGATAAAGTAGTTCGAAAAGTGGAGAGATT
ATGGGATGTTCGAGATAATAAGATAGTTCGTCACACTGTGTATCTCCTGGTAACGCCTCGTGTTGTTGAG
GAAGCACGAAAACATTTTGATTGTCCAGTTCTAGAGGGAATGGAACTTGAAAATCAAGGTGGTGTGGGCA
CTGAGCTCAACCATTGGGAAAAAAGGTTATTAGAGAATGAAGCGATGACTGGTTCTCACACTCAGAATCG
AGTACTCTCTCGAATCACTCTGGCATTAATGGAGGACACTGGCTGGTATAAAGCAAATTACAGCATGGCT
GAGAAGTTAGACTGGGGCCGAGGAATGGGCTGTGACTTTGTCAGGAAGAGCTGTAAATTCTGGATTGATC
AGCAGAGACAAAAGAGACAGATGCTGAGCCCTTACTGTGACACGCTCAGAAGTAACCCACTGCAGCTAAC
TTGCAGACAGGACCAGAGAGCAGTTGCCGTGTGTAATTTGCAGAAGTTCCCTAAGCCTTTACCACAGGAA
TACCAGTACTTTGATGAACTCAGTGGAATACCTGCAGAAGATTTGCCTTATTATGGTGGCTCCGTGGAAA
TTGCTGACTACTGCCCTTTCAGTCAGGAATTCAGTTGGCATTTAAGTGGTGAATATCAGCGCAGCTCAGA
TTGTAGAATATTGGAAAATCAACCAGAAATTTTTAAGAACTATGGCGCTGAAAAGTATGGACCTCATTCC
GTTTGTCTAATTCAGAAATCAGCATTCGTTATGGAGAAGTGTGAGAGGAAGCTGAGTTACCCAGACTGGG
GAAGCGGATGCTATCAGGTTTCTTGTTCTCCTCAAGGTCTGAAAGTTTGGGTCCAAGATACTTCATATTT
GTGTAGTCGGGCTGGGCAGGTCCTCCCTGTCAGTATCCAGATGAATGGCTGGATTCACGATGGAAACCTG
CTCTGCCCATCATGTTGGGACTTCTGTGAGCTCTGTCCTCCAGAAACAGATCCTCCAGCCACTAACCTGA
CCCGAGCTCTGCCACTTGATCTTTGTTCCTGTTCCTCGAGCCTGGTGGTCACCCTCTGGCTTCTGCTAGG
CAATCTGTTTCCTCTGCTGGCTGGATTTCTTCTGTGTATATGGCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227243 representing NM_001136049
Red=Cloning site Green=Tags(s)

MVTTLGPKMAAEWGGGVGYSGSGPGRSRWRWSGSVWVRSVLLLLGGLRASATSTPVSLGSSPPCRHHVPS
DTEVINKVHLKANHVVKRDVDEHLRIKTVYDKSVEELLPEKKNLVKNKLFPQAISYLEKTFQVRRPAGTI
LLSRQCATNQYLRKENDPHRYCTGECAAHTKCGPVIVPEEHLQQCRVYRGGKWPHGAVGVPDQEGISDAD
FVLYVGALATERCSHENIISYAAYCQQEANMDRPIAGYANLCPNMISTQPQEFVGMLSTVKHEVIHALGF
SAGLFAFYHDKDGNPLTSRFADGLPPFNYSLGLYQWSDKVVRKVERLWDVRDNKIVRHTVYLLVTPRVVE
EARKHFDCPVLEGMELENQGGVGTELNHWEKRLLENEAMTGSHTQNRVLSRITLALMEDTGWYKANYSMA
EKLDWGRGMGCDFVRKSCKFWIDQQRQKRQMLSPYCDTLRSNPLQLTCRQDQRAVAVCNLQKFPKPLPQE
YQYFDELSGIPAEDLPYYGGSVEIADYCPFSQEFSWHLSGEYQRSSDCRILENQPEIFKNYGAEKYGPHS
VCLIQKSAFVMEKCERKLSYPDWGSGCYQVSCSPQGLKVWVQDTSYLCSRAGQVLPVSIQMNGWIHDGNL
LCPSCWDFCELCPPETDPPATNLTRALPLDLCSCSSSLVVTLWLLLGNLFPLLAGFLLCIWH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001136049
ORF Size 2076 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001136049.2, NP_001129521.2
RefSeq ORF 2055 bp
Locus ID 89782
UniProt ID Q96KR4, B4DR62
Cytogenetics 3q29
Protein Families Druggable Genome, Protease, Transmembrane
MW 77.9 kDa
Summary This gene encodes a zinc-metallopeptidase. The encoded protein may play a role in cell migration and invasion. Studies of a similar protein in Drosophila indicate a potential role in mitotic progression. Alternatively spliced transcript variants have been described. provided by RefSeq, Feb 2009
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Documents
Resources
"NM_001136049" in other vectors (4)
SKU Description Size Price
RC227243L3 Lenti ORF clone of Human leishmanolysin-like (metallopeptidase M8 family) (LMLN), transcript variant 1, Myc-DDK-tagged 10 ug
$934.00
RC227243L4 Lenti ORF clone of Human leishmanolysin-like (metallopeptidase M8 family) (LMLN), transcript variant 1, mGFP tagged 10 ug
$934.00
RG227243 LMLN (tGFP-tagged) - Human leishmanolysin-like (metallopeptidase M8 family) (LMLN), transcript variant 1 10 ug
$834.00
SC326178 LMLN (untagged)-Human leishmanolysin-like (metallopeptidase M8 family) (LMLN), transcript variant 1 10 ug
$698.00

Plasmid Map for RC227243

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227243
Circular map for RC227243

file-alt Citations

Pathogen evasion of chemokine response through suppression of CXCL10
Antonia, Gibbs, Trahair et al
Front Cell Infect Microbiol (2019) 9, 280 DOI: 10.3389/fcimb.2019.00280
AI Summary: In this study, OriGene products contributed by providing the CXCL10 plasmid (NM_001565) with C-terminal Myc and Flag tags and a codon-optimized GP63 plasmid on the pcDNA3.1/Hygro backbone, which were essential for investigating the mechanism of CXCL10 suppression by pathogen-derived protease GP63.
Snippet: The CXCL10 plasmid was obtained from Origene (NM_001565), and contains C terminal Myc and Flag epitope tags. For GP63, a codon optimized plasmid was obtained from OriGene on the pcDNA3.1/Hygro plasmid backbone
Pathogen-encoded evasion of CXCL10 and T cell responses
Antonia, Alvarez, Trahair et al
bioRxiv (2019)
Snippet: …Gibbs1, Kelly J. 4 Pittman1, Alyson B. Barnes1, Jeffrey S. Smith2, Sudarshan Rajagopal2,3, J. Will 5 View as HTML;The CXCL10 plasmid was obtained from Origene (NM_001565), and contains C terminal Myc and Flag epitope tags. …
Showing 1-2 of 2 papers.
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