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CRISP2 (NM_001142417) Human Tagged ORF Clone

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SKU
RC227036
CRISP2 (Myc-DDK-tagged)-Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CRISP2
Synonyms CRISP-2; CT36; GAPDL5; TPX1; TSP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227036 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTTACTACCGGTGTTGTTTCTGGTTACTGTGCTGCTTCCATCTTTACCTGCAGAAGGAAAGGATC
CCGCTTTTACTGCTTTGTTAACCACCCAGTTGCAAGTGCAAAGGGAGATTGTAAATAAACACAATGAACT
AAGGAAAGCAGTCTCTCCACCTGCCAGTAACATGCTAAAGATGGAATGGAGCAGAGAGGTAACAACGAAT
GCCCAAAGGTGGGCAAACAAGTGCACTTTACAACATAGTGATCCAGAGGACCGCAAAACCAGTACAAGAT
GTGGTGAGAATCTCTATATGTCAAGTGACCCTACTTCCTGGTCTTCTGCAATCCAAAGCTGGTATGACGA
GATCCTAGATTTTGTCTATGGTGTAGGACCAAAGAGTCCCAATGCAGTTGTTGGACATTATACTCAGCTT
GTTTGGTACTCGACTTACCAGGTAGGCTGTGGAATTGCCTACTGTCCCAATCAAGATAGTCTAAAATACT
ACTATGTTTGCCAATATTGTCCTGCTGGTAATAATATGAATAGAAAGAATACCCCGTACCAACAAGGAAC
ACCTTGTGCCGGTTGCCCTGATGACTGTGACAAAGGACTATGCACCAATAGTTGCCAGTATCAAGATCTC
CTAAGTAACTGTGATTCCTTGAAGAATACAGCTGGCTGTGAACATGAGTTACTCAAGGAAAAGTGCAAGG
CTACTTGCCTATGTGAGAACAAAATTTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227036 protein sequence
Red=Cloning site Green=Tags(s)

MALLPVLFLVTVLLPSLPAEGKDPAFTALLTTQLQVQREIVNKHNELRKAVSPPASNMLKMEWSREVTTN
AQRWANKCTLQHSDPEDRKTSTRCGENLYMSSDPTSWSSAIQSWYDEILDFVYGVGPKSPNAVVGHYTQL
VWYSTYQVGCGIAYCPNQDSLKYYYVCQYCPAGNNMNRKNTPYQQGTPCAGCPDDCDKGLCTNSCQYQDL
LSNCDSLKNTAGCEHELLKEKCKATCLCENKIY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001142417
ORF Size 729 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001142417.3
RefSeq Size 1387 bp
RefSeq ORF 732 bp
Locus ID 7180
UniProt ID P16562
Cytogenetics 6p12.3
Protein Families Secreted Protein
MW 27.3 kDa
Summary May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001142417" in other vectors (4)
SKU Description Size Price
RC227036L3 Lenti-ORF clone of CRISP2 (Myc-DDK-tagged)-Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 4 10 ug
$600.00
RC227036L4 Lenti-ORF clone of CRISP2 (mGFP-tagged)-Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 4 10 ug
$600.00
RG227036 CRISP2 (tGFP-tagged) - Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 4 10 ug
$500.00
SC324826 CRISP2 (untagged)-Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 4 10 ug
$330.00

Plasmid Map for RC227036

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227036
Circular map for RC227036

file-alt Citations

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