PAS1C1 (LMNTD1) (NM_001145727) Human Tagged ORF Clone

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SKU
RC226918
IFLTD1 (Myc-DDK-tagged)-Human intermediate filament tail domain containing 1 (IFLTD1), transcript variant 3
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAS1C1
Synonyms IFLTD1; LMNARS1; PAS1C1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226918 representing NM_001145727
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACAGCCAGTGAGAGCAGGAGACATCGGTGTTTCCAGAGTCCAGACCCACCACAATGCCAGCCCCT
TCTCAGTTCCGAAGAAACAGGATGAATCACCCATGATTGGGGATGGAGAAGATTATTTCCTTTCTTTGTT
TGGTGATTCAAAGAAACTTACAGCACACTCAAACTACACTCAGAAAACTTTAAAATACTTTTCTATGATT
CTTGAAGAAGTTGGCCAATTTACCTCCAGTTCTCTTGGAGATGTTGAAATAGCTGAAGTGAATGTCAAGG
GTTTGTTCGTGAAGCTCATTAACTCTTCCCTTGACAAAGAAATGGCAATTGGAGATCATATTCTCCAGCA
AAATGTGAATGGACAAACCATTTCTTTGTACCGATTCCTTCCAAACATCGTAATGCAGGCAAATTCCACA
GTAACAGTGTGGGCAGCAGCATCTGAAGCAAAGCATCAACCTCCATCAGATTTTCTTTGGAAGGAACAAG
ACAAGTTTAGAGCAAGTCCTGATTGTATAACAATCCTGTGCAAACCGAACGGTCAAGCCATTGCGTGGTA
CACCCCTATCCACTGGAAGCAAGCGTGGGAAAAATTAGATGCTGACGTTGAATTTAACAGATGTTCAGTA
GTATCTCCAACATTCCGAAAGCGTGTGTTTCAGTGGACAGCATCTACAGCTACAATAACTAAAGAAAAAC
AAGATCAACCTAAGAAAGATATCTCAAATTATCAGGTGGAACAAGCTCAAGTTCTTCTTAAGAGAGAGAA
GGAAATCCCACCAACCGTTTTCCCTAATCGCAGCCCTTGGTGCCAGAATCCCTATGTCTCTGCACATCCT
TACTGTCCTCTGATTGAACCACACAATACATCCACCGCTGGAGGCAGATTGGATAGACAGCCCAGGACTC
GGTCAACCAGACCTAATCGAGCCTCAGGGTCTAAGAAAAAGAAGACATCTGAGTCACAAAAGCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC226918 representing NM_001145727
Red=Cloning site Green=Tags(s)

MQQPVRAGDIGVSRVQTHHNASPFSVPKKQDESPMIGDGEDYFLSLFGDSKKLTAHSNYTQKTLKYFSMI
LEEVGQFTSSSLGDVEIAEVNVKGLFVKLINSSLDKEMAIGDHILQQNVNGQTISLYRFLPNIVMQANST
VTVWAAASEAKHQPPSDFLWKEQDKFRASPDCITILCKPNGQAIAWYTPIHWKQAWEKLDADVEFNRCSV
VSPTFRKRVFQWTASTATITKEKQDQPKKDISNYQVEQAQVLLKREKEIPPTVFPNRSPWCQNPYVSAHP
YCPLIEPHNTSTAGGRLDRQPRTRSTRPNRASGSKKKKTSESQKQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001145727
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145727.3
RefSeq ORF 978 bp
Locus ID 160492
UniProt ID Q8N9Z9
Cytogenetics 12p12.1
MW 36.6 kDa
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Documents
Resources
"NM_001145727" in other vectors (4)
SKU Description Size Price
RC226918L3 Lenti ORF clone of Human intermediate filament tail domain containing 1 (IFLTD1), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC226918L4 Lenti ORF clone of Human intermediate filament tail domain containing 1 (IFLTD1), transcript variant 3, mGFP tagged 10 ug
$600.00
RG226918 IFLTD1 (tGFP-tagged) - Human intermediate filament tail domain containing 1 (IFLTD1), transcript variant 3 10 ug
$500.00
SC326560 IFLTD1 (untagged)-Human intermediate filament tail domain containing 1 (IFLTD1), transcript variant 3, mRNA 10 ug
$330.00

Plasmid Map for RC226918

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Cloning scheme for RC226918

Circular map for RC226918

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