RPS24 (NM_001142285) Human Tagged ORF Clone

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SKU
RC226724
RPS24 (Myc-DDK-tagged)-Human ribosomal protein S24 (RPS24), transcript variant d
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPS24
Synonyms DBA3; eS24; S24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226724 representing NM_001142285
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCAACCGACTACTTCAGAGGAAACAAATGG
TCATTGATGTCCTTCACCCCGGGAAGGCGACAGTGCCTAAGACAGAAATTCGGGAAAAACTAGCCAAAAT
GTACAAGACCACACCGGATGTCATCTTTGTATTTGGATTCAGAACTCATTTTGGTGGTGGCAAGACAACT
GGCTTTGGCATGATTTATGATTCCCTGGATTATGCAAAGAAAAATGAACCCAAACATAGACTTGCAAGAC
ATGGCCTGTATGAGAAGAAAAAGACCTCAAGAAAGCAACGAAAGGAACGCAAGAACAGAATGAAGAAAGT
CAGGGGGACTGCAAAGGCCAATGTTGGTGCTGGCAAAAAGATGAGGGAATTGGGGCTTGGAGTGCAAGCA
TTGGGAAGAATTTCCCAGGAAGAGAGATGCACAGATGTGAAGAACTCGAAGGCAAGAGAAAGCCGGGGGG
TTGTGTGGCAGGTAGAAGTGCCAGGACCGTGGAGCGTGTGGACATGTGGCAGATTGCGGAGGGGCTGTGG
CAAGTATTTACAGGTGGCCGTTACCTGGAGGAAGACTGAAAACAGAGAACAGTGCTGCCAGGCGTGCCTT
TTGGAGAGGGCACTGGTCAGAAACGGAGCCTTCATGTCGCCTGCCTCACCTGCTCCTGCTGGTTCTCCCC
ACCCTGTGGACGGTGACTTGGTCCTCCACTTGCCAGAAGCCTTGTCAGCAACCTTGACTCTGTCACCCCA
CATCCAGGCCATCAACAAGTCTTTTGGCCCTTTCTTTGAAATCCACCAGGAATCCAGCTGCTTCTCCCCA
CCTTCCTGCCTCTCTGGTCTGGGCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC226724 representing NM_001142285
Red=Cloning site Green=Tags(s)

MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTT
GFGMIYDSLDYAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKKMRELGLGVQA
LGRISQEERCTDVKNSKARESRGVVWQVEVPGPWSVWTCGRLRRGCGKYLQVAVTWRKTENREQCCQACL
LERALVRNGAFMSPASPAPAGSPHPVDGDLVLHLPEALSATLTLSPHIQAINKSFGPFFEIHQESSCFSP
PSCLSGLGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001142285
ORF Size 867 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001142285.2
RefSeq ORF 870 bp
Locus ID 6229
UniProt ID P62847
Cytogenetics 10q22.3
Protein Pathways Ribosome
MW 32.3 kDa
Summary Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S24E family of ribosomal proteins. It is located in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Mutations in this gene result in Diamond-Blackfan anemia. provided by RefSeq, Nov 2008
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Documents
Resources
"NM_001142285" in other vectors (4)
SKU Description Size Price
RC226724L3 Lenti ORF clone of Human ribosomal protein S24 (RPS24), transcript variant d, Myc-DDK-tagged 10 ug
$600.00
RC226724L4 Lenti ORF clone of Human ribosomal protein S24 (RPS24), transcript variant d, mGFP tagged 10 ug
$600.00
RG226724 RPS24 (tGFP-tagged) - Human ribosomal protein S24 (RPS24), transcript variant d 10 ug
$500.00
SC325715 RPS24 (untagged)-Human ribosomal protein S24 (RPS24), transcript variant d 10 ug
$330.00

Plasmid Map for RC226724

Download sequence for

Cloning scheme for RC226724

Circular map for RC226724

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