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ATPBD4 (DPH6) (NM_001141972) Human Tagged ORF Clone

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SKU
RC226708
ATPBD4 (Myc-DDK-tagged)-Human ATP binding domain 4 (ATPBD4), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ATPBD4 or NM_001141972"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ATPBD4
Synonyms ATPBD4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226708 representing NM_001141972
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGTCGCGGCTCTGATCAGTGGTGGGAAGGACAGCTGCTATAATATGATGCAGTGCATTGCTGCTG
GGCATCAGATCGTTGCTTTAGCAAATCTAAGACCAGCTGAAAACCAAGTGGGGTCTGATGAACTGGATAG
CTACATGTATCAGACAGTGGGGCACCATGCCATTGACTTGTATGCAGAAGCAATGGCTCTTCCCCTCTAT
CGCCGAACCATAAGAGGAAGGAGCTTGGATACAAGACAAGTGTACACCAAATGTGAAGGTGATGAGGTTG
AAGATCTCTATGAGCTTTTGAAACTTGTTAAGGGCATCACTAGAATGACCTTGCTTGCTGAATATGATGC
TCTGAATCTCCAAGATTTTCACATGCATTTGAAAGTGGGCAGCCAGGCGATTGTTTACAGGACTCCAAAT
GAACTGTGCACTCACAGCAAGTTTGATAAACACACATTTCCTCCTTTTATCAGTGAGATTGCAAAATGTG
AAGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC226708 representing NM_001141972
Red=Cloning site Green=Tags(s)

MRVAALISGGKDSCYNMMQCIAAGHQIVALANLRPAENQVGSDELDSYMYQTVGHHAIDLYAEAMALPLY
RRTIRGRSLDTRQVYTKCEGDEVEDLYELLKLVKGITRMTLLAEYDALNLQDFHMHLKVGSQAIVYRTPN
ELCTHSKFDKHTFPPFISEIAKCEV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001141972
ORF Size 495 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001141972.2
RefSeq ORF 498 bp
Locus ID 89978
UniProt ID Q7L8W6
Cytogenetics 15q14
MW 18.5 kDa
Summary Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor (EEF2) to diphthamide (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001141972" in other vectors (4)
SKU Description Size Price
RC226708L3 Lenti ORF clone of Human ATP binding domain 4 (ATPBD4), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC226708L4 Lenti ORF clone of Human ATP binding domain 4 (ATPBD4), transcript variant 2, mGFP tagged 10 ug
$450.00
RG226708 ATPBD4 (tGFP-tagged) - Human ATP binding domain 4 (ATPBD4), transcript variant 2 10 ug
$350.00
SC325546 ATPBD4 (untagged)-Human ATP binding domain 4 (ATPBD4), transcript variant 2 10 ug
$165.00

Plasmid Map for RC226708

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC226708
Circular map for RC226708

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