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GRAF (ARHGAP26) (NM_001135608) Human Tagged ORF Clone

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SKU
RC226597
ARHGAP26 (Myc-DDK-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $695.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GRAF
Synonyms GRAF; GRAF1; OPHN1L; OPHN1L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226597 representing NM_001135608
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTCCCAGCGCTCGAGTTCAGCGACTGCTGCCTCGATAGTCCGCACTTCCGAGAGACGCTCAAGT
CGCACGAAGCAGAGCTGGACAAGACCAACAAATTCATCAAGGAGCTCATCAAGGACGGGAAGTCACTCAT
AAGCGCGCTCAAGAATTTGTCTTCAGCGAAGCGGAAGTTTGCAGATTCCTTAAATGAATTTAAATTTCAG
TGCATAGGAGATGCAGAAACAGATGATGAGATGTGTATAGCAAGGTCTTTGCAGGAGTTTGCCACTGTCC
TCAGGAATCTTGAAGATGAACGGATACGGATGATTGAGAATGCCAGCGAGGTGCTCATCACTCCCTTGGA
GAAGTTTCGAAAGGAACAGATCGGGGCTGCCAAGGAAGCCAAAAAGAAGTATGACAAAGAGACAGAAAAG
TATTGTGGCATCTTAGAAAAACACTTGAATTTGTCTTCCAAAAAGAAAGAATCTCAGCTTCAGGAGGCAG
ACAGCCAAGTGGACCTGGTCCGGCAGCATTTCTATGAAGTATCCCTGGAATATGTCTTCAAGGTGCAGGA
AGTCCAAGAGAGAAAGATGTTTGAGTTTGTGGAGCCTCTGCTGGCCTTCCTGCAAGGACTCTTCACTTTC
TATCACCATGGTTACGAACTGGCCAAGGATTTCGGGGACTTCAAGACACAGTTAACCATTAGCATACAGA
ACACAAGAAATCGCTTTGAAGGCACTAGATCAGAAGTGGAATCACTGATGAAAAAGATGAAGGAGAATCC
CCTTGAGCACAAGACCATCAGTCCCTACACCATGGAGGGATACCTCTACGTGCAGGAGAAACGTCACTTT
GGAACTTCTTGGGTGAAGCACTACTGTACATATCAACGGGATTCCAAACAAATCACCATGGTACCATTTG
ACCAAAAGTCAGGAGGAAAAGGGGGAGAAGATGAATCAGTTATCCTCAAATCCTGCACACGGCGGAAAAC
AGACTCCATTGAGAAGAGGTTTTGCTTTGATGTGGAAGCAGTAGACAGGCCAGGGGTTATCACCATGCAA
GCTTTGTCGGAAGAGGACCGGAGGCTCTGGATGGAAGCCATGGATGGCCGGGAACCTGTCTACAACTCGA
ACAAAGACAGCCAGAGTGAAGGGACTGCGCAGTTGGACAGCATTGGCTTCAGCATAATCAGGAAATGCAT
CCATGCTGTGGAAACCAGAGGGATCAACGAGCAAGGGCTGTATCGAATTGTGGGGGTCAACTCCAGAGTG
CAGAAGTTGCTGAGTGTCCTGATGGACCCCAAGACTGCTTCTGAGACAGAAACAGATATCTGTGCTGAAT
GGGAGATAAAGACCATCACTAGTGCTCTGAAGACCTACCTAAGAATGCTTCCAGGACCACTCATGATGTA
CCAGTTTCAAAGAAGTTTCATCAAAGCAGCAAAACTGGAGAACCAGGAGTCTCGGGTCTCTGAAATCCAC
AGCCTTGTTCATCGGCTCCCAGAGAAAAATCGGCAGATGTTACAGCTGCTCATGAACCACTTGGCAAATG
TTGCTAACAACCACAAGCAGAATTTGATGACGGTGGCAAACCTTGGTGTGGTGTTTGGACCCACTCTGCT
GAGGCCTCAGGAAGAAACAGTAGCAGCCATCATGGACATCAAATTTCAGAACATTGTCATTGAGATCCTA
ATAGAAAACCACGAAAAGATATTTAACACCGTGCCCGATATGCCTCTCACCAATGCCCAGCTGCACCTGT
CTCGGAAGAAGAGCAGTGACTCCAAGCCCCCGTCCTGCAGCGAGAGGCCCCTGACGCTCTTCCACACCGT
TCAGTCAACAGAGAAACAGGAACAAAGGAACAGCATCATCAACTCCAGTTTGGAATCTGTCTCATCAAAT
CCAAACAGCATCCTTAATTCCAGCAGCAGCTTACAGCCCAACATGAACTCCAGTGACCCAGACCTGGCTG
TGGTCAAACCCACCCGGCCCAACTCACTCCCCCCGAATCCAAGCCCAACTTCACCCCTCTCGCCATCTTG
GCCCATGTTCTCGGCGCCATCCAGCCCTATGCCCACCTCATCCACGTCCAGCGACTCATCCCCCGTCAGC
ACACCGTTCCGGAAGGCAAAAGCCTTGTATGCCTGCAAAGCTGAACATGACTCAGAACTTTCGTTCACAG
CAGGCACGGTCTTCGATAATGTTCACCCATCTCAGGAGCCTGGCTGGTTGGAGGGGACTCTGAACGGAAA
GACTGGCCTCATCCCTGAGAATTACGTGGAGTTCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC226597 representing NM_001135608
Red=Cloning site Green=Tags(s)

MGLPALEFSDCCLDSPHFRETLKSHEAELDKTNKFIKELIKDGKSLISALKNLSSAKRKFADSLNEFKFQ
CIGDAETDDEMCIARSLQEFATVLRNLEDERIRMIENASEVLITPLEKFRKEQIGAAKEAKKKYDKETEK
YCGILEKHLNLSSKKKESQLQEADSQVDLVRQHFYEVSLEYVFKVQEVQERKMFEFVEPLLAFLQGLFTF
YHHGYELAKDFGDFKTQLTISIQNTRNRFEGTRSEVESLMKKMKENPLEHKTISPYTMEGYLYVQEKRHF
GTSWVKHYCTYQRDSKQITMVPFDQKSGGKGGEDESVILKSCTRRKTDSIEKRFCFDVEAVDRPGVITMQ
ALSEEDRRLWMEAMDGREPVYNSNKDSQSEGTAQLDSIGFSIIRKCIHAVETRGINEQGLYRIVGVNSRV
QKLLSVLMDPKTASETETDICAEWEIKTITSALKTYLRMLPGPLMMYQFQRSFIKAAKLENQESRVSEIH
SLVHRLPEKNRQMLQLLMNHLANVANNHKQNLMTVANLGVVFGPTLLRPQEETVAAIMDIKFQNIVIEIL
IENHEKIFNTVPDMPLTNAQLHLSRKKSSDSKPPSCSERPLTLFHTVQSTEKQEQRNSIINSSLESVSSN
PNSILNSSSSLQPNMNSSDPDLAVVKPTRPNSLPPNPSPTSPLSPSWPMFSAPSSPMPTSSTSSDSSPVS
TPFRKAKALYACKAEHDSELSFTAGTVFDNVHPSQEPGWLEGTLNGKTGLIPENYVEFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001135608
ORF Size 2277 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001135608.3
RefSeq ORF 2280 bp
Locus ID 23092
UniProt ID Q9UNA1, A0A0S2Z536
Cytogenetics 5q31.3
Protein Families Druggable Genome
MW 86 kDa
Summary Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2017
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Documents
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"NM_001135608" in other vectors (6)
SKU Description Size Price
RC226597L1 Lenti-ORF clone of ARHGAP26 (Myc-DDK-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 10 ug
$995.00
RC226597L2 Lenti-ORF clone of ARHGAP26 (mGFP-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 10 ug
$995.00
RC226597L3 Lenti-ORF clone of ARHGAP26 (Myc-DDK-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 10 ug
$995.00
RC226597L4 Lenti-ORF clone of ARHGAP26 (mGFP-tagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 10 ug
$995.00
RG226597 ARHGAP26 (tGFP-tagged) - Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 10 ug
$789.00 $895.00
SC325319 ARHGAP26 (untagged)-Human Rho GTPase activating protein 26 (ARHGAP26), transcript variant 2 10 ug
$765.00

Plasmid Map for RC226597

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC226597
Circular map for RC226597

file-alt Citations

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