R3HCC1 (NM_001136108) Human Tagged ORF Clone

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SKU
RC226560
R3HCC1 (Myc-DDK-tagged)-Human R3H domain and coiled-coil containing 1 (R3HCC1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol R3HCC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226560 representing NM_001136108
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTCAGGGAACAGAGGACCTAAAGGGCCCAGGACAAAGGTGTGAGAATGAGCCACTGCTGGACCCTG
TTGGCCCTGAGCCTCTGGGGCCTGAGAGTCAGTCAGGGAAGGGAGACATGGTGGAGATGGCCACACGGTT
TGGGTCCACCCTGCAGCTAGACCTGGAAAAGGGGAAGGAGAGTCTGTTGGAGAAGAGGCTGGTGGCAGAG
GAGGAAGAGGACGAAGAGGAGGTGGAAGAGGATGGCCCCAGCAGCTGCTCGGAGGACGATTACAGTGAGC
TGCTGCAGGAGATCACAGACAACCTGACGAAGAAGGAGATTCAGATAGAGAAGATCCATTTGGACACATC
CTCCTTCGTGGAGGAGCTGCCTGGAGAGAAGGACCTTGCCCACGTGGTAGAGATCTATGACTTTGAACCA
GCGCTCAAGACGGAGGACCTGCTGGCAACGTTTTCTGAGTTCCAAGAGAAGGGGTTCAGGATTCAGTGGG
TGGATGATACTCACGCACTCGGCATCTTTCCCTGCCTGGCCTCAGCTGCGGAAGCCCTGACCCGGGAGTT
CTCGGTGCTCAAGATCCGGCCCCTCACACAGGGAACCAAGCAGTCAAAGCTCAAAGCCTTGCAGAGGCCA
AAACTCCTGCGTCTGGTGAAGGAGAGGCCACAGACAAATGCGACTGTGGCCCGGCGGCTGGTGGCCCGGG
CCCTGGGACTCCAACACAAAAAGAAAGAGCGGCCTGCTGTCCGGGGTCCGCTGCCGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC226560 representing NM_001136108
Red=Cloning site Green=Tags(s)

MTQGTEDLKGPGQRCENEPLLDPVGPEPLGPESQSGKGDMVEMATRFGSTLQLDLEKGKESLLEKRLVAE
EEEDEEEVEEDGPSSCSEDDYSELLQEITDNLTKKEIQIEKIHLDTSSFVEELPGEKDLAHVVEIYDFEP
ALKTEDLLATFSEFQEKGFRIQWVDDTHALGIFPCLASAAEALTREFSVLKIRPLTQGTKQSKLKALQRP
KLLRLVKERPQTNATVARRLVARALGLQHKKKERPAVRGPLPP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001136108
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001136108.1, NP_001129580.1
RefSeq Size 1644 bp
RefSeq ORF 1323 bp
Locus ID 203069
UniProt ID Q9Y3T6
Cytogenetics 8p21.3
MW 28.3 kDa
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Documents
Resources
"NM_001136108" in other vectors (4)
SKU Description Size Price
RC226560L3 Lenti-ORF clone of R3HCC1 (Myc-DDK-tagged)-Human R3H domain and coiled-coil containing 1 (R3HCC1) 10 ug
$600.00
RC226560L4 Lenti-ORF clone of R3HCC1 (mGFP-tagged)-Human R3H domain and coiled-coil containing 1 (R3HCC1) 10 ug
$600.00
RG226560 R3HCC1 (tGFP-tagged) - Human R3H domain and coiled-coil containing 1 (R3HCC1) 10 ug
$500.00
SC324855 R3HCC1 (untagged)-Human R3H domain and coiled-coil containing 1 (R3HCC1) 10 ug
$503.00

Plasmid Map for RC226560

Download sequence for

Cloning scheme for RC226560

Circular map for RC226560

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