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PAN2 (NM_001127460) Human Tagged ORF Clone

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SKU
RC226359
PAN2 (Myc-DDK-tagged)-Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,046.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAN2
Synonyms USP52
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226359 representing NM_001127460
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTTTGAGGGTCTGGACCCTGGACTGGCAGAATATGCCCCAGCCATGCATTCTGCCCTGGACCCTG
TCTTGGATGCCCACCTGAACCCAAATCTGCTACAGAATGTGGAGCTGGACCCAGAGGGAGTGGCCTTGGA
GGCTCTTCCCGTCCAGGAATCAGTGCACATAATGGAAGGTGTCTACTCTGAATTGCACAGCGTGGTGGCT
GAAGTGGGTGTACCTGTTTCCGTCTCCCACTTTGACTTGCACGAGGAGATGCTGTGGGTGGGGAGCCACG
GGGGCCATGCCACTTCATTTTTTGGCCCAGCCTTGGAGCGCTACTCATCCTTTCAAGTCAATGGCAGTGA
TGATATTCGGCAGATCCAGAGCCTGGAGAATGGTATCCTTTTTCTCACCAAGAACAACCTCAAGTATATG
GCCCGTGGGGGCCTCATTATATTTGATTACCTGCTGGATGAGAATGAGGATATGCACAGTCTCCTACTGA
CTGACAGCAGCACTCTACTCGTTGGTGGGCTGCAGAATCACATACTAGAGATTGATCTTAACACTGTCCA
GGAGACTCAGAAGTATGCAGTAGAGACGCCTGGAGTCACCATCATGAGACAGACAAATCGCTTCTTCTTC
TGCGGCCACACGTCTGGCAAGGTTTCCCTGAGAGACCTCCGTACTTTTAAGGTGGAACATGAGTTTGATG
CCTTCTCAGGAAGTCTGTCAGACTTTGATGTGCATGGCAACCTGCTAGCTGCCTGTGGCTTCTCCAGCCG
CCTCACTGGCCTGGCCTGCGACCGTTTCCTCAAGGTGTATGATTTGCGCATGATGCGTGCCATCACACCA
CTTCAAGTACATGTGGATCCTGCCTTCTTGCGCTTCATTCCTACATATACTTCTCGTCTTGCTATCATCT
CTCAGTCAGGGCAGTGCCAATTCTGTGAACCCACAGGCCTGGCCAACCCAGCCGATATCTTTCATGTGAA
TCCTGTGGGGCCTCTGCTAATGACATTTGATGTGTCAGCCAGCAAGCAGGCTCTGGCCTTTGGGGATTCT
GAGGGCTGTGTGCACCTCTGGACTGATTCCCCGGAGCCTTCCTTCAACCCCTACTCCCGTGAGACTGAGT
TTGCTTTGCCGTGTCTCGTGGACTCACTGCCTCCTCTGGACTGGAGCCAGGACCTGCTGCCTCTTTCCCT
CATCCCTGTCCCACTCACCACTGACACACTTCTCTCTGATTGGCCTGCTGCCAACTCTGCTCCAGCTCCC
AGGCGAGCACCACCCGTGGATGCAGAGATTCTGCGCACCATGAAGAAGGTGGGCTTCATTGGCTATGCGC
CCAATCCCCGCACCAGGCTGCGCAATCAGATACCCTACAGACTCAAGGAGTCAGACAGTGAATTTGACAG
CTTCAGCCAGGTCACTGAGTCACCAGTAGGACGAGAAGAGGAACCACATCTCCACATGGTTTCTAAGAAA
TACCGCAAGGTGACCATCAAATATTCCAAGCTAGGGCTGGAGGACTTTGACTTCAAACACTACAATAAGA
CCTTGTTTGCTGGATTAGAGCCCCACATTCCCAACGCCTACTGTAACTGCATGATCCAGGTGCTCTATTT
CCTGGAGCCTGTACGCTGTCTAATTCAAAACCACCTTTGCCAGAAGGAGTTCTGTCTGGCATGTGAGCTG
GGCTTCCTGTTTCACATGTTGGACCTCTCTCGTGGTGACCCTTGCCAGGGCAATAATTTTCTTCGGGCAT
TCCGTACTATTCCTGAGGCCTCAGCCCTCGGTCTAATCCTGGCTGACTCAGATGAGGCCTCAGGCAAGGG
CAATCTGGCCAGGCTCATTCAGAGGTGGAATCGCTTCATTCTCACTCAACTGCATCAAGATATGCAGGAG
CTGGAAATACCACAGGCTTATCGAGGTGCTGGAGGCAGCAGCTTTTGCTCATCGGGGGACTCTGTTATTG
GGCAGCTCTTCAGCTGTGAGATGGAGAACTGCAGCCTCTGCCGCTGTGGCAGTGAGACCGTGCGAGCCTC
ATCCACTCTGCTTTTCACACTCTCCTACCCTGATGGTAGCAAAAGTGATAAAACTGGGAAGAACTATGAC
TTTGCTCAGGTGCTGAAGCGAAGCATCTGCCTGGACCAGAATACACAGGCCTGGTGTGACACCTGTGAAA
AGTACCAGCCCACGATTCAGACCCGCAACATCCGCCATCTGCCAGATATTCTTGTCATCAATTGTGAGGT
GAACAGCTCAAAAGAGGCTGATTTCTGGAGAATGCAGGCTGAGGTTGCCTTCAAGATGGCAGTAAAGAAA
CACGGTGGGGAAATCTCCAAGAACAAGGAATTTGCTTTGGCTGATTGGAAGGAACTAGGGAGTCCAGAGG
GTGTGCTGGTGTGTCCCTCCATTGAGGAGTTGAAGAACGTCTGGCTTCCTTTCTCCATTCGCATGAAGAT
GACCAAAAACAAAGGGCTGGATGTTTGCAATTGGACTGATGGGGATGAGATGCAGTGGGGCCCAGCCAGG
GCAGAGGAGGAGCATGGTGTCTATGTGTATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGCA
CAGGGGGCAGCCTGGTGGCTCACATCAAAGTTGGAGAGACCTACCACCAGCGCAAGGAGGGCGTTACTCA
CCAGCAGTGGTATCTGTTCAATGACTTTCTTATTGAACCTATTGATAAGCATGAAGCTGTGCAGTTTGAC
ATGAATTGGAAAGTACCTGCAATCCTTTATTATGTCAAACGGAATCTCAATTCCAGATACAACCTGAACA
TCAAGAACCCTATTGAGGCAAGTGTCTTGCTGGCTGAAGCCTCGCTGGCACGGAAGCAGCGGAAAACACA
TACTACCTTTATTCCACTGATGCTGAATGAGATGCCACAGATTGGGGACCTGGTGGGTCTGGATGCTGAG
TTTGTCACCCTTAATGAGGAGGAAGCAGAGTTACGCAGTGATGGTACCAAGTCTACCATTAAACCAAGCC
AGATGTCAGTAGCCAGGATTACCTGTGTTCGGGGCCAGGGACCCAATGAGGGTATCCCCTTCATTGATGA
CTACATCTCTACCCAGGAGCAGGTGGTGGATTACTTGACTCAATACTCGGGTATAAAGCCTGGTGACCTC
GATGCCAAAATTTCCTCCAAGCACCTAACAACTCTCAAGTCTACCTACTTAAAGCTTCGTTTTCTCATTG
ACATTGGAGTCAAGTTTGTGGGTCATGGCCTGCAGAAGGACTTCCGGGTCATCAACCTGATGGTGCCCAA
GGACCAAGTCCTTGACACTGTCTACCTGTTCCATATGCCCCGAAAACGAATGATTTCCCTGCGATTCCTT
GCTTGGTACTTTCTGGACCTGAAGATTCAAGGGGAAACCCATGACAGTATTGAGGATGCCCGCACAGCCC
TTCAGCTGTACCGAAAGTATCTGGAGCTAAGCAAAAATGGCACTGAGCCTGAGTCTTTCCACAAGGTGCT
CAAGGGTCTTTATGAGAAGGGCAGAAAGATGGACTGGAAGGTGCCTGAGCCTGAGGGCCAAACAAGTCCC
AAGAATGCAGCTGTCTTCTCCTCAGTGCTGGCGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC226359 representing NM_001127460
Red=Cloning site Green=Tags(s)

MNFEGLDPGLAEYAPAMHSALDPVLDAHLNPNLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVA
EVGVPVSVSHFDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGSDDIRQIQSLENGILFLTKNNLKYM
ARGGLIIFDYLLDENEDMHSLLLTDSSTLLVGGLQNHILEIDLNTVQETQKYAVETPGVTIMRQTNRFFF
CGHTSGKVSLRDLRTFKVEHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAITP
LQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLMTFDVSASKQALAFGDS
EGCVHLWTDSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDLLPLSLIPVPLTTDTLLSDWPAANSAPAP
RRAPPVDAEILRTMKKVGFIGYAPNPRTRLRNQIPYRLKESDSEFDSFSQVTESPVGREEEPHLHMVSKK
YRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACEL
GFLFHMLDLSRGDPCQGNNFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQLHQDMQE
LEIPQAYRGAGGSSFCSSGDSVIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPDGSKSDKTGKNYD
FAQVLKRSICLDQNTQAWCDTCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRMQAEVAFKMAVKK
HGGEISKNKEFALADWKELGSPEGVLVCPSIEELKNVWLPFSIRMKMTKNKGLDVCNWTDGDEMQWGPAR
AEEEHGVYVYDLMATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFD
MNWKVPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQIGDLVGLDAE
FVTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDL
DAKISSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFL
AWYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSP
KNAAVFSSVLAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001127460
ORF Size 3606 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001127460.3
RefSeq ORF 3609 bp
Locus ID 9924
UniProt ID Q504Q3
Cytogenetics 12q13.3
Protein Families Protease
MW 135.2 kDa
Summary This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants. provided by RefSeq, Oct 2009
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"NM_001127460" in other vectors (6)
SKU Description Size Price
RC226359L1 Lenti ORF clone of Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,346.00
RC226359L2 Lenti ORF clone of Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 1, mGFP tagged 10 ug
$1,346.00
RC226359L3 Lenti ORF clone of Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,346.00
RC226359L4 Lenti ORF clone of Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 1, mGFP tagged 10 ug
$1,346.00
RG226359 PAN2 (tGFP-tagged) - Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 1 10 ug
$1,246.00
SC323266 PAN2 (untagged)-Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 1 10 ug
$1,152.00

Plasmid Map for RC226359

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC226359
Circular map for RC226359

file-alt Citations

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