LIFR (NM_001127671) Human Tagged ORF Clone
SKU
RC226327
LIFR (Myc-DDK-tagged)-Human leukemia inhibitory factor receptor alpha (LIFR), transcript variant 1
$955.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LIFR |
| Synonyms | CD118; LIF-R; SJS2; STWS; SWS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001127671 |
| ORF Size | 3291 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001127671.2 |
| RefSeq ORF | 3294 bp |
| Locus ID | 3977 |
| UniProt ID | P42702 |
| Cytogenetics | 5p13.1 |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway |
| MW | 123.74 kDa |
| Summary | This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding two different isoforms have been found for this gene. provided by RefSeq, Jun 2018 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001127671" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC226327L3 | Lenti ORF clone of Human leukemia inhibitory factor receptor alpha (LIFR), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,255.00
|
|
| RC226327L4 | Lenti ORF clone of Human leukemia inhibitory factor receptor alpha (LIFR), transcript variant 1, mGFP tagged | 10 ug |
$1,255.00
|
|
| RG226327 | LIFR (tGFP-tagged) - Human leukemia inhibitory factor receptor alpha (LIFR), transcript variant 1 | 10 ug |
$789.00
$1,155.00
|
|
| SC323254 | LIFR (untagged)-Human leukemia inhibitory factor receptor alpha (LIFR), transcript variant 1 | 10 ug |
$956.00
|
|
Citations
LIFR antagonism reverses epithelial pro-CAF programs in pancreatic ductal adenocarcinoma
Giorgio, Sette, Sensini et al
Biochem Pharmacol (2026) 246, 117707 DOI: 10.1016/j.bcp.2026.117707
Giorgio, Sette, Sensini et al
Biochem Pharmacol (2026) 246, 117707 DOI: 10.1016/j.bcp.2026.117707
Cited by 1
AI Summary: OriGene products, specifically the human LIFR plasmid (RC226327) and IL6ST/CD130 plasmid (RC215123), were used to transfect cells and assess LIFR-dependent STAT3 activation, thereby facilitating the study of LIF:LIFR axis signaling in pancreatic ductal adenocarcinoma.
Snippet: Extracellular matrix remodelling that occurs in pancreatic ductal adenocarcinoma (PDAC) is considered a promoting factor of cancer growth, immune evasion and;To evaluate LIFR-dependent STAT3 activation, HEPG2 cells were seeded into 24-well plates at 7.5 × 104 cells/well and incubated for 24 h. Cells were then co-transfected with plasmids encoding human LIFR (100 ng;OriGene RC226327), IL6ST/CD130 (100 ng;OriGene RC215123),
Exploring Boswellia serrata Triterpenes: A New Frontier in Leukemia Inhibitory Factor Receptor Modulation
Finamore, Festa, Cammarota et al
ACS Omega (2025) 10 (21), 22269-22284 DOI: 10.1021/acsomega.5c03492
Finamore, Festa, Cammarota et al
ACS Omega (2025) 10 (21), 22269-22284 DOI: 10.1021/acsomega.5c03492
Cited by 7
AI Summary: In this study, OriGene products hLIFR (CAT no. RC226327) and CD130 (IL6ST) (CAT no. RC215123) expression vectors were used to transiently transfect cells for functional assays investigating the inhibitory effects of Boswellia serrata triterpenes on leukemia inhibitory factor receptor (LIFR) signaling.
Snippet: … E4041 Promega, Madison, WI, USA), a vector encoding the hLIFR (CAT no. RC226327) and CD130 (IL6ST) (CAT no. RC215123, OriGene Technologies, Inc. …
Bile acids serve as endogenous antagonists of the Leukemia inhibitory factor (LIF) receptor in oncogenesis
Di Giorgio, Morretta, Lupia et al
Biochem Pharmacol (2024) 223, 116134 DOI: 10.1016/j.bcp.2024.116134
Di Giorgio, Morretta, Lupia et al
Biochem Pharmacol (2024) 223, 116134 DOI: 10.1016/j.bcp.2024.116134
Cited by 20
Repositioning Mifepristone as a Leukaemia Inhibitory Factor Receptor Antagonist for the Treatment of Pancreatic Adenocarcinoma
Di Giorgio, Lupia, Marchianò et al
Cells (2022) 11 (21) DOI: 10.3390/cells11213482
Di Giorgio, Lupia, Marchianò et al
Cells (2022) 11 (21) DOI: 10.3390/cells11213482
Cited by 17
AI Summary: OriGene products, specifically the hLIFR expression plasmid (CAT# RC226327) and the CD130 (IL6ST) expression plasmid (CAT# RC215123), were used to enable overexpression of LIFR and its co-receptor in cell-based assays investigating mifepristone's antagonistic effects on LIFR signaling in pancreatic cancer cells.
Snippet: d with the reporter plasmid pGL4.47[luc2P/SIE/Hygro] (200 ng) (CAT#: E4041 Promega, Madison, WI, USA), a vector encoding the hLIFR (CAT# RC226327) (100 ng) and CD130 (IL6ST) (100 ng) (CAT#: RC215123, OriGene Technologies, Inc. Rockville, MD, USA), and finally a vector encoding the human RENILLA luciferase gene (pGL4.70) (100 ng) (Promega, Madison, WI, USA). On day 2, cells were primed with the cyt
MicroRNA-125a influences breast cancer stem cells by targeting leukemia inhibitory factor receptor which regulates the Hippo signaling pathway
Nandy, Arumugam, Subramani et al
Oncotarget (2015) 6 (19), 17366-78 DOI: 10.18632/oncotarget.3953
Nandy, Arumugam, Subramani et al
Oncotarget (2015) 6 (19), 17366-78 DOI: 10.18632/oncotarget.3953
Cited by 70
AI Summary: In this study, OriGene’s Human LIFR cDNA clone (RC223307) and LIFR shRNA plasmids (TL309741) were used to modulate LIFR expression, enabling the authors to investigate how miR-125a regulates breast cancer stem cells via the Hippo signaling pathway.
Showing 1-5 of 5 papers.
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