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SPINK5 (NM_001127698) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC226324
SPINK5 (Myc-DDK-tagged)-Human serine peptidase inhibitor, Kazal type 5 (SPINK5), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $952.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPINK5
Synonyms LEKTI; LETKI; NETS; NS; VAKTI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226324 representing NM_001127698
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATAGCCACAGTGTCAGTGCTTCTGCCCTTGGCTCTTTGCCTCATACAAGATGCTGCCAGTAAGA
ATGAAGATCAGGAAATGTGCCATGAATTTCAGGCATTTATGAAAAATGGAAAACTGTTCTGTCCCCAGGA
TAAGAAATTTTTTCAAAGTCTTGATGGAATAATGTTCATCAATAAATGTGCCACGTGCAAAATGATACTG
GAAAAAGAAGCAAAATCACAGAAGAGGGCCAGGCATTTAGCAAGAGCTCCCAAGGCTACTGCCCCAACAG
AGCTGAATTGTGATGATTTTAAAAAAGGAGAAAGAGATGGGGATTTTATCTGTCCTGATTATTATGAAGC
TGTTTGTGGCACAGATGGGAAAACATATGACAACAGATGTGCACTGTGTGCTGAGAATGCGAAAACCGGG
TCCCAAATTGGTGTAAAAAGTGAAGGGGAATGTAAGAGCAGTAATCCAGAGCAGGATGTATGCAGTGCTT
TTCGGCCCTTTGTTAGAGATGGAAGACTTGGATGCACAAGGGAAAATGATCCTGTTCTTGGTCCTGATGG
GAAGACGCATGGCAATAAGTGTGCAATGTGTGCTGAGCTGTTTTTAAAAGAAGCTGAAAATGCCAAGCGA
GAGGGTGAAACTAGAATTCGACGAAATGCTGAAAAGGATTTTTGCAAGGAATATGAAAAACAAGTGAGAA
ATGGAAGGCTTTTTTGTACACGGGAGAGTGATCCAGTCCGTGGCCCTGACGGCAGGATGCATGGCAACAA
ATGTGCCCTGTGTGCTGAAATTTTCAAGCAGCGTTTTTCAGAGGAAAACAGTAAAACAGATCAAAATTTG
GGAAAAGCTGAAGAAAAAACTAAAGTTAAAAGAGAAATTGTGAAACTCTGCAGTCAATATCAAAATCAGG
CAAAGAATGGAATACTTTTCTGTACCAGAGAAAATGACCCTATTCGTGGTCCAGATGGGAAAATGCATGG
CAACTTGTGTTCCATGTGTCAAGCCTACTTCCAAGCAGAAAATGAAGAAAAGAAAAAGGCTGAAGCACGA
GCTAGAAACAAAAGAGAATCTGGAAAAGCAACCTCATATGCAGAGCTTTGCAGTGAATATCGAAAGCTTG
TGAGGAACGGAAAACTTGCTTGCACCAGAGAGAACGATCCTATCCAGGGCCCAGATGGGAAAGTGCATGG
CAACACCTGCTCCATGTGTGAGGTCTTCTTCCAAGCAGAAGAAGAAGAAAAGAAAAAGAAGGAAGGTAAA
TCAAGAAACAAAAGACAATCTAAGAGTACAGCTTCCTTTGAGGAGTTGTGTAGTGAATACCGCAAATCCA
GGAAAAACGGACGGCTTTTTTGCACCAGAGAGAATGACCCCATCCAGGGCCCAGATGGAAAAATGCATGG
CAACACCTGCTCCATGTGTGAGGCCTTCTTTCAACAAGAAGAAAGAGCAAGAGCAAAGGCTAAAAGAGAA
GCTGCAAAGGAAATCTGCAGTGAATTTCGGGACCAAGTGAGGAATGGAACACTTATATGCACCAGGGAGC
ATAATCCTGTCCGTGGCCCAGATGGCAAAATGCATGGAAACAAGTGTGCCATGTGTGCCAGTGTGTTCAA
ACTTGAAGAAGAAGAGAAGAAAAATGATAAAGAAGAAAAAGGGAAAGTCGAGGCTGAAAAAGTTAAGAGA
GAAGCAGTTCAGGAGCTGTGCAGTGAATATCGTCATTATGTGAGGAATGGACGACTCCCCTGTACCAGAG
AGAATGATCCTATTGAGGGTCTAGATGGGAAAATCCACGGCAACACCTGCTCCATGTGTGAAGCCTTCTT
CCAGCAAGAAGCAAAAGAAAAAGAAAGAGCTGAACCCAGAGCAAAAGTCAAAAGAGAAGCTGAAAAGGAG
ACATGCGATGAATTTCGGAGACTTTTGCAAAATGGAAAACTTTTCTGCACAAGAGAAAATGATCCTGTGC
GTGGCCCAGATGGCAAGACCCATGGCAACAAGTGTGCCATGTGTAAGGCAGTCTTCCAGAAAGAAAATGA
GGAAAGAAAGAGGAAAGAAGAGGAAGATCAGAGAAATGCTGCAGGACATGGTTCCAGTGGTGGTGGAGGA
GGAAACACTCAGGACGAATGTGCTGAGTATCGGGAACAAATGAAAAATGGAAGACTCAGCTGTACTCGGG
AGAGTGATCCTGTACGTGATGCTGATGGCAAATCGTACAACAATCAGTGTACCATGTGTAAAGCAAAATT
GGAAAGAGAAGCAGAGAGAAAAAATGAGTATTCTCGCTCCAGATCAAATGGGACTGGATCAGAATCAGGG
AAGGATACATGTGATGAGTTTAGAAGCCAAATGAAAAATGGAAAACTCATCTGCACTCGAGAAAGTGACC
CTGTCCGGGGTCCAGATGGCAAGACACATGGCAATAAGTGTACTATGTGTAAGGAAAAACTGGAAAGGGA
AGCAGCTGAAAAAAAAAAGAAAGAGGATGAAGACAGGAGCAATACAGGAGAAAGGAGCAATACAGGAGAA
AGGAGCAATGACAAAGAGGATCTGTGTCGTGAATTTCGAAGCATGCAGAGAAATGGAAAGCTTATCTGCA
CCAGAGAAAATAACCCTGTTCGAGGCCCATATGGCAAGATGCACATCAATAAATGTGCTATGTGTCAGAG
CATCTTTGATCGAGAAGCTAATGAAAGAAAAAAGAAAGATGAAGAGAAATCAAGTAGCAAGCCCTCAAAT
AATGCAAAGGACCAGTGCAGACAGGTTCAGAATGAAGCGGAGGATGCAAAATTTAGACAACCTGGGCGTT
CCTTGGCCTCTGTTGCCAGGATGAGTACAGATGAGTGCAGTGAATTTCGAAACTATATAAGGAACAATGA
ACTCATCTGCCCTAGAGAGAATGACCCAGTGCACGGTGCTGATGGAAAGTTCTATACAAACAAGTGCTAC
ATGTGCAGAGCTGTCTTTCTAACAGAAGCTTTGGAAAGGGCAAAGCTTCAAGAAAAGCCATCCCATGTTA
GAGCTTCTCAAGAGGAAGACAGCCCAGACTCTTTCAGTTCTCTGGATTCTGAGATGTGCAAAGACTACCG
AGTATTGCCCAGGATAGGTTATCTTTGTCCAAAGGATTTAAAGCCTGTCTGTGGTGACGATGGCCAAACC
TACAACAATCCTTGCATGCTCTGTCATGAAAACCTGATACGCCAAACAAATACACACATCCGCAGTACAG
GGAAGTGTGAGGAGAGCAGCACCCCAGGAACCACCGCAGCCAGCATGCCCCCGTCTGACGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC226324 representing NM_001127698
Red=Cloning site Green=Tags(s)

MKIATVSVLLPLALCLIQDAASKNEDQEMCHEFQAFMKNGKLFCPQDKKFFQSLDGIMFINKCATCKMIL
EKEAKSQKRARHLARAPKATAPTELNCDDFKKGERDGDFICPDYYEAVCGTDGKTYDNRCALCAENAKTG
SQIGVKSEGECKSSNPEQDVCSAFRPFVRDGRLGCTRENDPVLGPDGKTHGNKCAMCAELFLKEAENAKR
EGETRIRRNAEKDFCKEYEKQVRNGRLFCTRESDPVRGPDGRMHGNKCALCAEIFKQRFSEENSKTDQNL
GKAEEKTKVKREIVKLCSQYQNQAKNGILFCTRENDPIRGPDGKMHGNLCSMCQAYFQAENEEKKKAEAR
ARNKRESGKATSYAELCSEYRKLVRNGKLACTRENDPIQGPDGKVHGNTCSMCEVFFQAEEEEKKKKEGK
SRNKRQSKSTASFEELCSEYRKSRKNGRLFCTRENDPIQGPDGKMHGNTCSMCEAFFQQEERARAKAKRE
AAKEICSEFRDQVRNGTLICTREHNPVRGPDGKMHGNKCAMCASVFKLEEEEKKNDKEEKGKVEAEKVKR
EAVQELCSEYRHYVRNGRLPCTRENDPIEGLDGKIHGNTCSMCEAFFQQEAKEKERAEPRAKVKREAEKE
TCDEFRRLLQNGKLFCTRENDPVRGPDGKTHGNKCAMCKAVFQKENEERKRKEEEDQRNAAGHGSSGGGG
GNTQDECAEYREQMKNGRLSCTRESDPVRDADGKSYNNQCTMCKAKLEREAERKNEYSRSRSNGTGSESG
KDTCDEFRSQMKNGKLICTRESDPVRGPDGKTHGNKCTMCKEKLEREAAEKKKKEDEDRSNTGERSNTGE
RSNDKEDLCREFRSMQRNGKLICTRENNPVRGPYGKMHINKCAMCQSIFDREANERKKKDEEKSSSKPSN
NAKDQCRQVQNEAEDAKFRQPGRSLASVARMSTDECSEFRNYIRNNELICPRENDPVHGADGKFYTNKCY
MCRAVFLTEALERAKLQEKPSHVRASQEEDSPDSFSSLDSEMCKDYRVLPRIGYLCPKDLKPVCGDDGQT
YNNPCMLCHENLIRQTNTHIRSTGKCEESSTPGTTAASMPPSDE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001127698
ORF Size 3282 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001127698.2
RefSeq ORF 3285 bp
Locus ID 11005
UniProt ID Q9NQ38
Cytogenetics 5q32
MW 124.08 kDa
Summary This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2015
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Documents
Resources
"NM_001127698" in other vectors (4)
SKU Description Size Price
RC226324L3 Lenti-ORF clone of SPINK5 (Myc-DDK-tagged)-Human serine peptidase inhibitor, Kazal type 5 (SPINK5), transcript variant 1 10 ug
$1,252.00
RC226324L4 Lenti-ORF clone of SPINK5 (mGFP-tagged)-Human serine peptidase inhibitor, Kazal type 5 (SPINK5), transcript variant 1 10 ug
$1,252.00
RG226324 SPINK5 (tGFP-tagged) - Human serine peptidase inhibitor, Kazal type 5 (SPINK5), transcript variant 1 10 ug
$1,152.00
SC323253 SPINK5 (untagged)-Human serine peptidase inhibitor, Kazal type 5 (SPINK5), transcript variant 1 10 ug
$1,048.00

Plasmid Map for RC226324

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC226324
Circular map for RC226324

file-alt Citations

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