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PABPC1L (NM_001124756) Human Tagged ORF Clone

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SKU
RC226022
PABPC1L (Myc-DDK-tagged)-Human poly(A) binding protein, cytoplasmic 1-like (PABPC1L)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $572.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PABPC1L
Synonyms C20orf119; dJ1069P2.3; EPAB; PABPC1L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226022 representing NM_001124756
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGCCAGCGGTTCTGGCTACCCGCTTGCCTCGCTTTACGTGGGCGATCTGCACCCCGACGTGACCG
AGGCCATGCTCTATGAGAAGTTCTCTCCCGCCGGCCCCATCCTGTCCATCCGCGTGTGCCGCGATGTAGC
CACCCGGCGCTCGCTGGGCTACGCCTACATCAACTTCCAGCAGCCCGCGGACGCGGAGCGGGCACTGGAC
ACAATGAACTTTGAGATGCTCAAAGGCCAGCCTATTCGCATCATGTGGTCCCAGCGAGACCCAGGACTTC
GCAAGTCAGGTGTGGGCAACATCTTCATCAAGAACCTGGAGGACTCCATTGACAACAAGGCTTTATATGA
TACCTTCTCCACCTTTGGGAACATCCTCTCTTGCAAGGTGGCGTGTGACGAGCATGGCTCCCGGGGTTTC
GGCTTTGTCCATTTTGAGACCCATGAGGCCGCACAGCAGGCCATCAACACCATGAATGGGATGCTGCTGA
ATGACCGCAAAGTCTTTGTGGGTCACTTCAAGTCTCGACGGGAGCGGGAGGCGGAGCTGGGGGCGCGGGC
CCTGGAGTTCACCAACATCTACGTGAAGAACCTCCCGGTGGATGTGGACGAGCAAGGCCTGCAGGACCTC
TTCTCCCAGTTTGGGAAAATGCTGAGTGTGAAGGTGATGAGGGACAACAGCGGCCACTCGCGGTGCTTTG
GCTTTGTCAACTTTGAGAAGCATGAGGAAGCCCAGAAGGCCGTGGTCCATATGAACGGGAAGGAGGTGAG
CGGGCGGCTGCTGTACGCGGGCCGGGCCCAAAAGCGCGTGGAGCGGCAGAATGAACTGAAGCGCAGGTTT
GAGCAGATGAAGCAGGACCGGCTGAGGCGTTACCAGGGTGTGAACTTGTATGTGAAGAATCTGGACGACT
CCATTGATGACGACAAACTGAGGAAAGAGTTCTCTCCCTATGGAGTAATTACCAGTGCGAAGGTGATGAC
AGAGGGTGGCCACAGCAAGGGGTTTGGCTTTGTGTGTTTTTCCTCCCCAGAAGAGGCGACAAAGGCCGTG
ACAGAGATGAACGGGCGCATCGTGGGCACCAAGCCACTCTACGTGGCACTGGCCCAGCGCAAAGAGGAGC
GGAAGGCCATCTTGACCAACCAGTACATGCAGCGCCTCTCCACCATGCGGACCCTGAGCAACCCCCTCCT
GGGCTCCTTTCAGCAGCCCTCCAGCTACTTCCTGCCTGCCATGCCCCAGCCTCCAGCCCAGGCTGCATAC
TATGGCTGTGGCCCAGTGACACCCACCCAGCCTGCCCCCAGGTGGACATCCCAGCCACCTAGACCTTCCT
GTGCCTCAATGGTCCGGCCACCAGTTGTGCCTCGGCGCCCCCCGGCCCACATCAGCAGTGTCAGGCAGGC
CTCCACCCAGGTGCCACGCACGGTGCCTCATACCCAGAGAGTAGCCAACATTGGTACTCAGACCACAGGA
CCCAGTGGGGTAGGATGCTGTACACCAGGCCGGCCGCTCCTGCCGTGCAAATGTTCCTCAGCAGCACATA
GCACCTATCGGGTCCAGGAGCCGGCTGTGCACATCCCAGGACAGGAGCCCCTGACCGCGTCCATGCTGGC
TGCGGCGCCCCTGCATGAGCAAAAGCAGATGATTGGGGAGCGTCTCTACCCCCTTATCCATGATGTCCAC
ACCCAGCTGGCTGGCAAGATCACGGGCATGCTGCTGGAGATTGACAACTCAGAGCTGTTGCTCATGCTGG
AGTCTCCAGAATCCCTCCATGCCAAGATAGACGAGGCAGTGGCCGTGCTGCAGGCACACCAGGCTATGGA
GCAGCCGAAGGCGTACATGCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC226022 representing NM_001124756
Red=Cloning site Green=Tags(s)

MNASGSGYPLASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDVATRRSLGYAYINFQQPADAERALD
TMNFEMLKGQPIRIMWSQRDPGLRKSGVGNIFIKNLEDSIDNKALYDTFSTFGNILSCKVACDEHGSRGF
GFVHFETHEAAQQAINTMNGMLLNDRKVFVGHFKSRREREAELGARALEFTNIYVKNLPVDVDEQGLQDL
FSQFGKMLSVKVMRDNSGHSRCFGFVNFEKHEEAQKAVVHMNGKEVSGRLLYAGRAQKRVERQNELKRRF
EQMKQDRLRRYQGVNLYVKNLDDSIDDDKLRKEFSPYGVITSAKVMTEGGHSKGFGFVCFSSPEEATKAV
TEMNGRIVGTKPLYVALAQRKEERKAILTNQYMQRLSTMRTLSNPLLGSFQQPSSYFLPAMPQPPAQAAY
YGCGPVTPTQPAPRWTSQPPRPSCASMVRPPVVPRRPPAHISSVRQASTQVPRTVPHTQRVANIGTQTTG
PSGVGCCTPGRPLLPCKCSSAAHSTYRVQEPAVHIPGQEPLTASMLAAAPLHEQKQMIGERLYPLIHDVH
TQLAGKITGMLLEIDNSELLLMLESPESLHAKIDEAVAVLQAHQAMEQPKAYMH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001124756
ORF Size 1842 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001124756.2, NP_001118228.1
RefSeq ORF 1845 bp
Locus ID 80336
UniProt ID Q4VY17
Cytogenetics 20q13.12
MW 68.2 kDa
Summary This gene belongs to the polyadenylate-binding protein type-1 family of proteins. Members of this family bind to the polyA tails of mRNAs to regulate mRNA stability and translation. The mouse ortholog of this gene is required for female fertility. In human, expression of a functional protein is regulated by alternative splicing. The protein-coding splice variant for this gene is abundantly expressed in human oocytes, while a noncoding splice variant subject to nonsense-mediated decay is the predominant splice variant expressed in somatic tissues. provided by RefSeq, Aug 2019
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"NM_001124756" in other vectors (6)
SKU Description Size Price
RC226022L1 Lenti ORF clone of Human poly(A) binding protein, cytoplasmic 1-like (PABPC1L), Myc-DDK-tagged 10 ug
$872.00
RC226022L2 Lenti ORF clone of Human poly(A) binding protein, cytoplasmic 1-like (PABPC1L), mGFP tagged 10 ug
$872.00
RC226022L3 Lenti ORF clone of Human poly(A) binding protein, cytoplasmic 1-like (PABPC1L), Myc-DDK-tagged 10 ug
$872.00
RC226022L4 Lenti ORF clone of Human poly(A) binding protein, cytoplasmic 1-like (PABPC1L), mGFP tagged 10 ug
$872.00
RG226022 PABPC1L (tGFP-tagged) - Human poly(A) binding protein, cytoplasmic 1-like (PABPC1L) 10 ug
$772.00
SC323154 PABPC1L (untagged)-Human poly(A) binding protein, cytoplasmic 1-like (PABPC1L) 10 ug
$630.00

Plasmid Map for RC226022

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC226022
Circular map for RC226022

file-alt Citations

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