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Upstream Binding Protein 1 (UBP1) (NM_001128160) Human Tagged ORF Clone

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SKU
RC225866
UBP1 (Myc-DDK-tagged)-Human upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $469.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Upstream Binding Protein 1
Synonyms LBP-1a; LBP-1B; LBP1A; LBP1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC225866 representing NM_001128160
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTGGGTGCTCAAGATGGACGAGGTGATCGAGTCCGGGCTGGTGCACGACTTCGACGCCAGCCTCT
CGGGCATCGGGCAGGAACTGGGCGCCGGCGCTTACAGCATGAGTGATGTCTTGGCATTGCCCATTTTCAA
GCAGGAAGATTCCAGCCTTCCATTGGATGGTGAAACAGAGCACCCACCCTTTCAGTATGTGATGTGTGCT
GCAACGTCACCAGCAGTAAAACTGCATGATGAAACGCTTACTTATTTGAACCAAGGTCAGTCATATGAAA
TTCGGATGCTGGATAATCGGAAAATGGGTGATATGCCTGAGATCAATGGAAAATTAGTAAAGAGCATCAT
AAGGGTTGTATTCCATGACAGACGGCTACAATACACAGAGCATCAGCAACTTGAAGGATGGAAGTGGAAT
CGCCCAGGAGACAGACTTCTTGATTTAGATATTCCAATGTCTGTGGGAATAATTGACACAAGGACGAATC
CAAGCCAGTTAAATGCGGTTGAATTTCTGTGGGACCCAGCAAAACGCACCTCTGCTTTCATTCAGGTACA
CTGCATCAGCACAGAATTTACTCCACGGAAGCACGGAGGTGAAAAGGGAGTGCCCTTTAGGATCCAGGTT
GACACCTTTAAGCAGAATGAAAATGGAGAATACACAGATCATCTACACTCAGCTAGCTGCCAAATCAAAG
TTTTTAAGCCTAAAGGTGCAGACAGGAAACAAAAAACTGACCGAGAGAAGATGGAGAAGAGAACAGCTCA
TGAAAAAGAAAAGTATCAGCCGTCCTATGATACCACAATCCTCACAGAGTGTTCTCCGTGGCCCGATGCC
CCCACAGCCTATGTGAATAACAGCCCTTCCCCAGCGCCCACTTTCACCTCCCCACAGCAGAGCACTTGCA
GTGTCCCAGACAGCAATTCTTCTTCCCCAAATCATCAGGGAGATGGAGCTTCACAGACCTCTGGTGAACA
AATTCAGCCTTCAGCTACGATCCAGGAAACACAGCAATGGCTGCTCAAAAACAGATTCTCTTCCTACACA
AGACTGTTCTCTAATTTTTCAGGTGCCGACTTATTAAAACTGACAAAGGAGGATTTAGTTCAAATTTGTG
GTGCAGCCGATGGAATTCGGCTCTATAATTCACTGAAGTCAAGGTCGGTTAGACCCCGTTTAACCATCTA
TGTCTGCCGGGAGCAGCCAAGCAGCACAGTGCTGCAAGGGCAGCAGCAAGCTGCAAGCAGTGCAAGCGAG
AATGGCAGTGGGGCACCCTATGTTTATCATGCAATCTACTTGGAAGAAATGATTGCCTCAGAAGTTGCTC
GAAAACTTGCGCTGGTGTTTAATATCCCTCTCCACCAAATTAATCAGGTTTACAGACAGGGTCCCACCGG
TATTCACATTCTTGTTAGTGATCAGATGGTTCAGAATTTTCAAGATGAGAGTTGTTTTTTATTCTCCACA
GTAAAAGCTGAAAGTAGTGATGGCATCCACATAATTTTGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC225866 representing NM_001128160
Red=Cloning site Green=Tags(s)

MAWVLKMDEVIESGLVHDFDASLSGIGQELGAGAYSMSDVLALPIFKQEDSSLPLDGETEHPPFQYVMCA
ATSPAVKLHDETLTYLNQGQSYEIRMLDNRKMGDMPEINGKLVKSIIRVVFHDRRLQYTEHQQLEGWKWN
RPGDRLLDLDIPMSVGIIDTRTNPSQLNAVEFLWDPAKRTSAFIQVHCISTEFTPRKHGGEKGVPFRIQV
DTFKQNENGEYTDHLHSASCQIKVFKPKGADRKQKTDREKMEKRTAHEKEKYQPSYDTTILTECSPWPDA
PTAYVNNSPSPAPTFTSPQQSTCSVPDSNSSSPNHQGDGASQTSGEQIQPSATIQETQQWLLKNRFSSYT
RLFSNFSGADLLKLTKEDLVQICGAADGIRLYNSLKSRSVRPRLTIYVCREQPSSTVLQGQQQAASSASE
NGSGAPYVYHAIYLEEMIASEVARKLALVFNIPLHQINQVYRQGPTGIHILVSDQMVQNFQDESCFLFST
VKAESSDGIHIILK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001128160
ORF Size 1512 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001128160.1, NP_001121632.1
RefSeq ORF 1515 bp
Locus ID 7342
UniProt ID Q9NZI7, A0A024R2J0
Cytogenetics 3p22.3
Protein Families Transcription Factors
MW 56.3 kDa
Summary Functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001128160" in other vectors (4)
SKU Description Size Price
RC225866L3 Lenti-ORF clone of UBP1 (Myc-DDK-tagged)-Human upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 2 10 ug
$769.00
RC225866L4 Lenti-ORF clone of UBP1 (mGFP-tagged)-Human upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 2 10 ug
$769.00
RG225866 UBP1 (tGFP-tagged) - Human upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 2 10 ug
$669.00
SC323111 UBP1 (untagged)-Human upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 2 10 ug
$517.00

Plasmid Map for RC225866

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC225866
Circular map for RC225866

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