NDRG1 (NM_001135242) Human Tagged ORF Clone
SKU
RC225609
NDRG1 (Myc-DDK-tagged)-Human N-myc downstream regulated 1 (NDRG1), transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NDRG1 |
| Synonyms | CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001135242 |
| ORF Size | 1182 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001135242.2 |
| RefSeq Size | 3520 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 10397 |
| UniProt ID | Q92597 |
| Cytogenetics | 8q24.22 |
| MW | 42.8 kDa |
| Summary | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, May 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001135242" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC225609L1 | Lenti ORF clone of Human N-myc downstream regulated 1 (NDRG1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC225609L2 | Lenti ORF clone of Human N-myc downstream regulated 1 (NDRG1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC225609L3 | Lenti ORF clone of Human N-myc downstream regulated 1 (NDRG1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC225609L4 | Lenti ORF clone of Human N-myc downstream regulated 1 (NDRG1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG225609 | NDRG1 (tGFP-tagged) - Human N-myc downstream regulated 1 (NDRG1), transcript variant 1 | 10 ug |
$886.00
|
|
| SC325042 | NDRG1 (untagged)-Human N-myc downstream regulated 1 (NDRG1), transcript variant 1 | 10 ug |
$686.00
|
|
Citations
ASCT2 is a major contributor to serine uptake in cancer cells
Conger, Chidley, Ozgurses et al
Cell Rep (2024) 43 (8), 114552 DOI: 10.1016/j.celrep.2024.114552
Conger, Chidley, Ozgurses et al
Cell Rep (2024) 43 (8), 114552 DOI: 10.1016/j.celrep.2024.114552
Cited by 26
NDRG1 overcomes resistance to immunotherapy of pancreatic ductal adenocarcinoma through inhibiting ATG9A-dependent degradation of MHC-1
Zhang, Song, Wei et al
Drug Resist Updat (2024) 73, 101040 DOI: 10.1016/j.drup.2023.101040
Zhang, Song, Wei et al
Drug Resist Updat (2024) 73, 101040 DOI: 10.1016/j.drup.2023.101040
Cited by 26
Usefulness of an immunohistochemical score in advanced pancreatic neuroendocrine tumors treated with CAPTEM or everolimus
Viúdez, Crespo, Gómez Dorronsoro et al
Pancreatology (2020) 21 (1), 215-223 DOI: 10.1016/j.pan.2020.12.009
Viúdez, Crespo, Gómez Dorronsoro et al
Pancreatology (2020) 21 (1), 215-223 DOI: 10.1016/j.pan.2020.12.009
Cited by 4
AI Summary: OriGene contributed to this study by providing pCMV6 vectors for NDRG-1 and PHLDA-3 cDNA overexpression, which were used to generate cell lines for functional analysis of these genes in pancreatic neuroendocrine tumors.
Snippet: pCMV6 vectors for NDRG-1 or PHLDA-3 cDNA overexpression were purchased from Origene. Cells were transfected with the corresponding cDNA or an empty vector as a control, and selected with hygromycin until established clones were selected. Colony formation
The proneural gene ASCL1 governs the transcriptional subgroup affiliation in glioblastoma stem cells by directly repressing the mesenchymal gene NDRG1
Narayanan, Gagliardi, Gallotti et al
Cell Death Differ. (2018) 26 (9), 1813-1831 DOI: 10.1038/s41418-018-0248-7
Narayanan, Gagliardi, Gallotti et al
Cell Death Differ. (2018) 26 (9), 1813-1831 DOI: 10.1038/s41418-018-0248-7
AI Summary: OriGene's NDRG1 cDNA was used in this study to generate lentiviral constructs for overexpression experiments, enabling the researchers to investigate the role of NDRG1 in glioblastoma stem cell differentiation and subgroup specification.
Snippet: cDNAs for human ASCL1 (Upstate, Lake Placid, NY, USA) and NDRG1 (Origene, Rockville, MD, USA) were cloned into the pC.sin.cPPT.PGK.GFP.WPRE11 monocistronic transfer lentiviral vector (LV) in place of the GFP sequence
N-Myc Downstream-Regulated Gene 1 Restricts Hepatitis C Virus Propagation by Regulating Lipid Droplet Biogenesis and Viral Assembly
Schweitzer, Zhang, Boyer et al
J. Virol. (2017)
Schweitzer, Zhang, Boyer et al
J. Virol. (2017)
SET-mediated NDRG1 inhibition is involved in acquisition of epithelial-to-mesenchymal transition phenotype and cisplatin resistance in human lung cancer cell
Liu, Gu, Yin et al
Cell. Signal. (2014)
Liu, Gu, Yin et al
Cell. Signal. (2014)
Cited by 35
Showing 1-6 of 6 papers.
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