CCDC160 (NM_001101357) Human Tagged ORF Clone

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SKU
RC225468
CCDC160 (Myc-DDK-tagged)-Human coiled-coil domain containing 160 (CCDC160)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC160
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC225468 representing NM_001101357
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCTAGAAGAAAACACTGGAAGGAGAATATGTTTACTCCTTTTTTTAGTGCACAAGATGTTCTAG
AAGAGACTTCTGAGCCTGAATCTTCTTCTGAACAAACGACTGCAGATAGCAGCAAGGGAATGGAAGAAAT
TTATAATTTGTCCAGTAGAAAGTTTCAGGAAGAAAGTAAATTTAAGAGGAAAAAATATATTTTCCAACTA
AATGAAATAGAACAAGAACAAAATTTAAGAGAGAACAAGAGAAACATTTCAAAGAATGAAACAGACACAA
ATTCTGCATCCTATGAATCATCTAATGTGGATGTTACAACAGAAGAAAGCTTTAACAGCACAGAAGATAA
CTCTACCTGCAGTACAGATAACTTACCAGCTCTACTAAGACAAGACATAAGAAAGAAATTTATGGAAAGA
ATGTCTCCAAAACTTTGCCTGAATCTTTTGAATGAAGAACTGGAAGAACTTAATATGAAATACAGAAAAA
TAGAAGAGGAATTTGAAAATGCTGAAAAAGAACTTTTGCACTACAAAAAAGAAATATTCACAAAACCCCT
AAATTTTCAAGAAACAGAGACGGATGCTTCAAAAAGTGACTATGAACTTCAAGCTTTAAGAAATGACCTG
TCTGAAAAAGCAACAAATGTAAAAAACTTAAGTGAACAGCTCCAGCAAGCCAAAGAAGTCATCCACAAAT
TGAACCTAGAGAACAGAAATTTAAAAGAAGCTGTTAGGAAGTTAAAGCATCAAACCGAGGTTGGAAATGT
GCTCCTAAAAGAAGAAATGAAATCATATTATGAATTAGAAATGGCAAAGATCCGCGGAGAGCTCAGTGTC
ATCAAGAATGAACTGAGAACTGAGAAGACCCTACAAGCAAGAAATAACAGAGCCTTGGAGTTGCTTAGAA
AATACTATGCTTCTTCAATGGTAACATCATCAAGTATCCTTGACCACTTTACTGGGGATTTTTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC225468 representing NM_001101357
Red=Cloning site Green=Tags(s)

MDARRKHWKENMFTPFFSAQDVLEETSEPESSSEQTTADSSKGMEEIYNLSSRKFQEESKFKRKKYIFQL
NEIEQEQNLRENKRNISKNETDTNSASYESSNVDVTTEESFNSTEDNSTCSTDNLPALLRQDIRKKFMER
MSPKLCLNLLNEELEELNMKYRKIEEEFENAEKELLHYKKEIFTKPLNFQETETDASKSDYELQALRNDL
SEKATNVKNLSEQLQQAKEVIHKLNLENRNLKEAVRKLKHQTEVGNVLLKEEMKSYYELEMAKIRGELSV
IKNELRTEKTLQARNNRALELLRKYYASSMVTSSSILDHFTGDFF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001101357
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001101357.2
RefSeq ORF 978 bp
Locus ID 347475
UniProt ID A6NGH7
Cytogenetics Xq26.2
MW 38.1 kDa
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Documents
Resources
"NM_001101357" in other vectors (4)
SKU Description Size Price
RC225468L3 Lenti ORF clone of Human coiled-coil domain containing 160 (CCDC160), Myc-DDK-tagged 10 ug
$600.00
RC225468L4 Lenti ORF clone of Human coiled-coil domain containing 160 (CCDC160), mGFP tagged 10 ug
$600.00
RG225468 CCDC160 (tGFP-tagged) - Human coiled-coil domain containing 160 (CCDC160) 10 ug
$500.00
SC317064 CCDC160 (untagged)-Human coiled-coil domain containing 160 (CCDC160) 10 ug
$330.00

Plasmid Map for RC225468

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Cloning scheme for RC225468

Circular map for RC225468

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