TEN1 (NM_001113324) Human Tagged ORF Clone

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SKU
RC225063
TEN1 (Myc-DDK-tagged)-Human chromosome 17 open reading frame 106 (C17orf106)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TEN1
Synonyms C17orf106
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC225063 representing NM_001113324
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCTGCCCAAACCTGGGACCTATTACCTCCCCTGGGAGGTTAGTGCAGGCCAAGTTCCTGATGGGA
GCACGCTGAGAACATTTGGCAGGTTGTGCCTCTATGACATGATTCAGTCCAGAGTAACACTGATGGCTCA
GCACGGATCCGATCAGCACCAGGTTCTTGTCTGTACCAAGTTGGTGGAGCCCTTCCACGCCCAGGTGGGC
TCCCTGTACATCGTCCTCGGGGAGCTCCAGCATCAGCAGGACAGAGGCTCCGTGGTGAAGGCGCGCGTGC
TGACCTGTGTGGAGGGGATGAACCTGCCCTTGTTGGAACAAGCCATCCGGGAGCAGAGACTGTACAAGCA
GGAGCGGGGCGGCAGCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC225063 representing NM_001113324
Red=Cloning site Green=Tags(s)

MMLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKLVEPFHAQVG
SLYIVLGELQHQQDRGSVVKARVLTCVEGMNLPLLEQAIREQRLYKQERGGSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001113324
ORF Size 369 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001113324.3
RefSeq Size 1010 bp
RefSeq ORF 372 bp
Locus ID 100134934
UniProt ID Q86WV5
Cytogenetics 17q25.1
MW 13.9 kDa
Summary C17ORF106, or TEN1, appears to function in a telomere-associated complex with STN1 (OBFC1; MIM 613128) and CTC1 (C17ORF68; MIM 613129) (Miyake et al., 2009 PubMed 19854130).supplied by OMIM, Nov 2009
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Documents
Resources
"NM_001113324" in other vectors (4)
SKU Description Size Price
RC225063L3 Lenti-ORF clone of TEN1 (Myc-DDK-tagged)-Human chromosome 17 open reading frame 106 (C17orf106) 10 ug
$450.00
RC225063L4 Lenti-ORF clone of TEN1 (mGFP-tagged)-Human chromosome 17 open reading frame 106 (C17orf106) 10 ug
$450.00
RG225063 TEN1 (tGFP-tagged) - Human chromosome 17 open reading frame 106 (C17orf106) 10 ug
$350.00
SC318765 TEN1 (untagged)-Human chromosome 17 open reading frame 106 (C17orf106) 10 ug
$165.00

Plasmid Map for RC225063

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Cloning scheme for RC225063

Circular map for RC225063

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