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MPP5 (NM_022474) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC224752L2
Lenti ORF clone of Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5), mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $918.00
3 Weeks*
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Other products for "MPP5 or NM_022474"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MPP5
Synonyms PALS1
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC224752).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022474
ORF Size 2025 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022474.2
RefSeq Size 5608 bp
RefSeq ORF 2028 bp
Locus ID 64398
UniProt ID Q8N3R9, Q658X5
Cytogenetics 14q23.3
Domains Guanylate_kin, GuKc, L27, PDZ, SH3
Protein Families Druggable Genome
Protein Pathways Tight junction
MW 77.3 kDa
Summary This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Feb 2012
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"NM_022474" in other vectors (7)
SKU Description Size Price
RC224752 MPP5 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5) 10 ug
$618.00
RC224752L1 Lenti ORF clone of Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5), Myc-DDK-tagged 10 ug
$918.00
RC224752L3 Lenti ORF clone of Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5), Myc-DDK-tagged 10 ug
$918.00
RC224752L4 Lenti ORF clone of Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5), mGFP tagged 10 ug
$918.00
RG224752 MPP5 (tGFP-tagged) - Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5) 10 ug
$789.00 $818.00
SC112491 MPP5 (untagged)-Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5) 10 ug
$469.00
SC318145 MPP5 (untagged)-Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5) 10 ug
$681.00

Plasmid Map for RC224752L2

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Cloning scheme for RC224752L2
Circular map for RC224752L2

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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