MUC13 (NM_033049) Human Tagged ORF Clone

  • TrueORF®
SKU
RC224663
MUC13 (Myc-DDK-tagged)-Human mucin 13, cell surface associated (MUC13)
  $713.00
2 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MUC13
Synonyms DRCC1; MUC-13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224663 representing NM_033049
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGCCATCATTCATCTTACTCTTCTTGCTCTCCTTTCTGTAAACACAGCCACCAACCAAGGCAACT
CAGCTGATGCTGTAACAACCACAGAAACTGCGACTAGTGGTCCTACAGTAGCTGCAGCTGATACCACTGA
AACTAATTTCCCTGAAACTGCTAGCACCACAGCAAATACACCTTTCCCAACAGCTACTTCACCTGCTCCC
CCCATAATTAGTACACATAGTTCCTCCACAATTCCTACACCTGCTCCCCCCATAATTAGTACACATAGTT
CCTCCACAATTCCTATACCTACTGCTGCAGACAGTGAGTCAACCACAAATGTAAATTCATTAGCTACCTC
TGACATAATCACCGCTTCATCTCCAAATGATGGATTAATCACAATGGTTCCTTCTGAAACACAAAGTAAC
AATGAAATGTCCCCCACCACAGAAGACAATCAATCATCAGGGCCTCCCACTGGCACCGCTTTATTGGAGA
CCAGCACCCTAAACAGCACAGGTCCCAGCAATCCTTGCCAAGATGATCCCTGTGCAGATAATTCGTTATG
TGTTAAGCTGCATAATACAAGTTTTTGCCTGTGTTTAGAAGGGTATTACTACAACTCTTCTACATGTAAG
AAAGGAAAGGTATTCCCTGGGAAGATTTCAGTGACAGTATCAGAAACATTTGACCCAGAAGAGAAACATT
CCATGGCCTATCAAGACTTGCATAGTGAAATTACTAGCTTGTTTAAAGATGTATTTGGCACATCTGTTTA
TGGACAGACTGTAATTCTTACTGTAAGCACATCTCTGTCACCAAGATCTGAAATGCGTGCTGATGACAAG
TTTGTTAATGTAACAATAGTAACAATTTTGGCAGAAACCACAAGTGACAATGAGAAGACTGTGACTGAGA
AAATTAATAAAGCAATTAGAAGTAGCTCAAGCAACTTTCTAAACTATGATTTGACCCTTCGGTGTGATTA
TTATGGCTGTAACCAGACTGCGGATGACTGCCTCAATGGTTTAGCATGCGATTGCAAATCTGACCTGCAA
AGGCCTAACCCACAGAGCCCTTTCTGCGTTGCTTCCAGTCTCAAGTGTCCTGATGCCTGCAACGCACAGC
ACAAGCAATGCTTAATAAAGAAGAGTGGTGGGGCCCCTGAGTGTGCGTGCGTGCCCGGCTACCAGGAAGA
TGCTAATGGGAACTGCCAAAAGTGTGCATTTGGCTACAGTGGACTCGACTGTAAGGACAAATTTCAGCTG
ATCCTCACTATTGTGGGCACCATCGCTGGCATTGTCATTCTCAGCATGATAATTGCATTGATTGTCACAG
CAAGATCAAATAACAAAACGAAGCATATTGAAGAAGAGAACTTGATTGACGAAGACTTTCAAAATCTAAA
ACTGCGGTCGACAGGCTTCACCAATCTTGGAGCAGAAGGGAGCGTCTTTCCTAAGGTCAGGATAACGGCC
TCCAGAGACAGCCAGATGCAAAATCCCTATTCAAGACACAGCAGCATGCCCCGCCCTGACTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC224663 representing NM_033049
Red=Cloning site Green=Tags(s)

MKAIIHLTLLALLSVNTATNQGNSADAVTTTETATSGPTVAAADTTETNFPETASTTANTPFPTATSPAP
PIISTHSSSTIPTPAPPIISTHSSSTIPIPTAADSESTTNVNSLATSDIITASSPNDGLITMVPSETQSN
NEMSPTTEDNQSSGPPTGTALLETSTLNSTGPSNPCQDDPCADNSLCVKLHNTSFCLCLEGYYYNSSTCK
KGKVFPGKISVTVSETFDPEEKHSMAYQDLHSEITSLFKDVFGTSVYGQTVILTVSTSLSPRSEMRADDK
FVNVTIVTILAETTSDNEKTVTEKINKAIRSSSSNFLNYDLTLRCDYYGCNQTADDCLNGLACDCKSDLQ
RPNPQSPFCVASSLKCPDACNAQHKQCLIKKSGGAPECACVPGYQEDANGNCQKCAFGYSGLDCKDKFQL
ILTIVGTIAGIVILSMIIALIVTARSNNKTKHIEEENLIDEDFQNLKLRSTGFTNLGAEGSVFPKVRITA
SRDSQMQNPYSRHSSMPRPDY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033049
ORF Size 1533 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033049.2, NP_149038.2
RefSeq Size 2961 bp
RefSeq ORF 1539 bp
Locus ID 56667
UniProt ID Q9H3R2
Cytogenetics 3q21.2
Protein Families Transmembrane
MW 54.3 kDa
Summary Epithelial mucins, such as MUC13, are a family of secreted and cell surface glycoproteins expressed by ductal and glandular epithelial tissues (Williams et al., 2001 PubMed 11278439).supplied by OMIM, Jul 2008
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Documents
Resources
"NM_033049" in other vectors (6)
SKU Description Size Price
RC224663L1 Lenti ORF clone of Human mucin 13, cell surface associated (MUC13), Myc-DDK-tagged 10 ug
$1,013.00
RC224663L2 Lenti ORF clone of Human mucin 13, cell surface associated (MUC13), mGFP tagged 10 ug
$1,013.00
RC224663L3 Lenti ORF clone of Human mucin 13, cell surface associated (MUC13), Myc-DDK-tagged 10 ug
$1,013.00
RC224663L4 Lenti ORF clone of Human mucin 13, cell surface associated (MUC13), mGFP tagged 10 ug
$1,013.00
RG224663 MUC13 (tGFP-tagged) - Human mucin 13, cell surface associated (MUC13) 10 ug
$913.00
SC305540 MUC13 (untagged)-Human mucin 13, cell surface associated (MUC13) 10 ug
$715.00

Plasmid Map for RC224663

Download sequence for

Cloning scheme for RC224663

Circular map for RC224663

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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