GR6 (LINC01565) (NM_007354) Human Tagged ORF Clone

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SKU
RC224627
C3orf27 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 27 (C3orf27)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GR6
Synonyms C3orf27; GR6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224627 representing NM_007354
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAAGCGCTCCATCAGATAGTGGTCCGCTGCAGTGAGCTGGTTTCCAGCACGAGCCTGCCTAGAC
TCAGCGTCTCCCGCCTCCAGGGACCCCCAGACTCTCAGCCCCTAGGCACCCTGGGCCAGGGTGGTTGGAA
GCTTCTAGGCATTGTGGGGTCTCTGGCACCAGAGACACTCGGGGGTCTGGGGACCGAGTTTGGGCCCTGT
ACCCACCCACTACCATTTGACATGGTGAGAGAGAGAGAGAGAGATGACGAGCTCAGGCAGGGATGGCTTC
TCCAGTGCCCACAATGTGCCAGGACTCTGCTGTGCCACTGTGGGCCTTTCCTCACCCCTCCCTCCCAGAC
ATCAAGCTCTGGTTTCCAGCTGTGTTCCTTGAAGCCGTCGGGCTCTCTGGTGACAGCCACAGAGCCTCTG
AGCAACTTTGCTTTCAGCTATTTTCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC224627 representing NM_007354
Red=Cloning site Green=Tags(s)

MKEALHQIVVRCSELVSSTSLPRLSVSRLQGPPDSQPLGTLGQGGWKLLGIVGSLAPETLGGLGTEFGPC
THPLPFDMVRERERDDELRQGWLLQCPQCARTLLCHCGPFLTPPSQTSSSGFQLCSLKPSGSLVTATEPL
SNFAFSYFP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007354
ORF Size 447 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007354.2, NP_031380.1
RefSeq Size 2700 bp
RefSeq ORF 450 bp
Locus ID 23434
Cytogenetics 3q21.3
MW 15.9 kDa
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Documents
Resources
"NM_007354" in other vectors (4)
SKU Description Size Price
RC224627L3 Lenti ORF clone of Human chromosome 3 open reading frame 27 (C3orf27), Myc-DDK-tagged 10 ug
$450.00
RC224627L4 Lenti ORF clone of Human chromosome 3 open reading frame 27 (C3orf27), mGFP tagged 10 ug
$450.00
RG224627 C3orf27 (tGFP-tagged) - Human chromosome 3 open reading frame 27 (C3orf27) 10 ug
$350.00
SC303919 C3orf27 (untagged)-Human chromosome 3 open reading frame 27 (C3orf27) 10 ug
$165.00

Plasmid Map for RC224627

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Cloning scheme for RC224627

Circular map for RC224627

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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