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VPS18 (NM_020857) Human Tagged ORF Clone

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SKU
RC224536
VPS18 (Myc-DDK-tagged)-Human vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $891.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VPS18
Synonyms PEP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224536 representing NM_020857
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCATCCTGGATGAGTACGAGAACTCGCTGTCCCGCTCGGCCGTCTTGCAGCCCGGCTGCCCTA
GCGTGGGCATCCCCCACTCGGGGTATGTGAATGCCCAGCTGGAGAAGGAAGTGCCCATCTTCACAAAGCA
GCGCATTGACTTCACCCCTTCCGAGCGCATTACCAGTCTTGTCGTCTCCAGCAATCAGCTGTGCATGAGC
CTGGGCAAGGATACACTGCTCCGCATTGACTTGGGCAAGGCAAATGAGCCCAACCACGTGGAGCTGGGAC
GTAAGGATGACGCAAAAGTTCACAAGATGTTCCTTGACCATACTGGCTCTCACCTGCTGATTGCCCTGAG
CAGCACGGAGGTCCTCTACGTGAACCGAAATGGACAGAAGGTACGGCCACTAGCACGCTGGAAGGGGCAG
CTGGTGGAGAGTGTGGGTTGGAACAAGGCACTGGGCACGGAGAGCAGCACAGGCCCCATCCTGGTCGGGA
CTGCCCAAGGCCACATCTTTGAAGCAGAGCTCTCAGCCAGCGAAGGTGGGCTTTTCGGCCCTGCTCCGGA
TCTCTACTTCCGCCCATTGTACGTGCTAAATGAAGAAGGGGGTCCAGCACCTGTGTGCTCCCTTGAGGCC
GAGCGGGGCCCTGATGGGCGTAGCTTTGTTATTGCCACCACTCGGCAGCGCCTCTTCCAGTTCATAGGCC
GAGCAGCAGAGGGGGCTGAGGCCCAGGGTTTCTCAGGGCTCTTTGCAGCTTACACGGACCACCCACCCCC
ATTCCGTGAGTTTCCCAGCAACCTGGGCTACAGTGAGTTGGCCTTCTACACCCCCAAGCTGCGCTCCGCA
CCCCGGGCCTTCGCCTGGATGATGGGGGATGGTGTGTTGTATGGGGCATTGGACTGTGGGCGCCCTGACT
CTCTGCTGAGCGAGGAGCGAGTCTGGGAGTACCCAGAGGGGGTAGGGCCTGGGGCCAGCCCACCCCTAGC
CATCGTCTTGACCCAGTTCCACTTCCTGCTGCTACTGGCAGACCGGGTGGAGGCAGTGTGCACACTGACC
GGGCAGGTGGTGCTGCGGGATCACTTCCTGGAGAAATTTGGGCCGCTGAAGCACATGGTGAAGGACTCCT
CCACAGGCCAGCTGTGGGCCTACACTGAGCGGGCTGTCTTCCGCTACCACGTGCAACGGGAGGCCCGAGA
TGTCTGGCGCACCTATCTGGACATGAACCGCTTCGATCTGGCCAAAGAGTATTGTCGAGAGCGGCCCGAC
TGCCTGGACACGGTCCTGGCCCGGGAGGCCGATTTCTGCTTTCGCCAGCGTCGCTACCTGGAGAGCGCAC
GCTGCTATGCCCTGACCCAGAGCTACTTTGAGGAGATTGCCCTCAAGTTCCTGGAGGCCCGACAGGAGGA
GGCTCTGGCTGAGTTCCTGCAGCGAAAACTGGCCAGTTTGAAGCCAGCCGAACGTACCCAGGCCACACTG
CTGACCACCTGGCTGACAGAGCTCTACCTGAGCCGGCTTGGGGCTCTGCAGGGCGACCCAGAGGCCCTGA
CTCTCTACCGAGAAACCAAGGAATGCTTTCGAACCTTCCTCAGCAGCCCCCGCCACAAAGAGTGGCTCTT
TGCCAGCCGGGCCTCTATCCATGAGCTGCTCGCCAGTCATGGGGACACAGAACACATGGTGTACTTTGCA
GTGATCATGCAGGACTATGAGCGGGTGGTGGCTTACCACTGTCAGCACGAGGCCTACGAGGAGGCCCTGG
CCGTGCTCGCCCGCCACCGTGACCCCCAGCTCTTCTACAAGTTCTCACCCATCCTCATCCGTCACATCCC
CCGCCAGCTTGTAGATGCCTGGATTGAGATGGGCAGCCGGCTGGATGCTCGTCAGCTCATTCCTGCCCTG
GTGAACTACAGCCAGGGTGGTGAGGTCCAGCAGGTGAGCCAGGCCATCCGCTACATGGAGTTCTGCGTGA
ACGTGCTGGGGGAGACTGAGCAGGCCATCCACAACTACCTGCTGTCACTGTATGCCCGTGGCCGGCCGGA
CTCACTACTGGCCTATCTGGAGCAGGCTGGGGCCAGCCCCCACCGGGTGCATTACGACCTCAAGTATGCG
CTGCGGCTCTGCGCCGAGCATGGCCACCACCGCGCTTGTGTCCATGTCTACAAGGTCCTAGAGCTGTATG
AGGAGGCCGTGGACCTGGCCCTGCAGGTGGATGTGGACCTGGCCAAGCAGTGTGCAGACCTGCCTGAGGA
GGATGAGGAATTGCGCAAGAAGCTGTGGCTGAAGATCGCACGGCACGTGGTGCAGGAAGAGGAAGATGTA
CAGACAGCCATGGCTTGCCTGGCTAGCTGCCCCTTGCTCAAGATTGAGGATGTGCTGCCCTTCTTTCCTG
ATTTCGTCACCATCGACCACTTCAAGGAGGCGATCTGCAGCTCACTTAAGGCCTACAACCACCACATCCA
GGAGCTGCAGCGGGAGATGGAAGAGGCTACAGCCAGTGCCCAGCGCATCCGGCGAGACCTGCAGGAGCTG
CGGGGCCGCTACGGCACTGTGGAGCCCCAGGACAAATGTGCCACCTGCGACTTCCCCCTGCTCAACCGCC
CTTTTTACCTCTTCCTCTGTGGCCATATGTTCCATGCTGACTGCCTGCTGCAGGCTGTGCGACCTGGCCT
GCCAGCCTACAAGCAGGCCCGGCTGGAGGAGCTGCAGAGGAAGCTGGGGGCTGCTCCACCCCCAGCCAAG
GGCTCTGCCCGGGCCAAGGAGGCCGAGGGTGGGGCTGCCACGGCAGGGCCCAGCCGGGAACAGCTCAAGG
CTGACCTGGATGAGTTGGTGGCCGCTGAGTGTGTGTACTGTGGGGAGCTGATGATCCGCTCTATCGACCG
GCCGTTCATCGACCCCCAGCGCTACGAGGAGGAGCAGCTCAGTTGGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224536 representing NM_020857
Red=Cloning site Green=Tags(s)

MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMS
LGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQ
LVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEA
ERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSA
PRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLT
GQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPD
CLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATL
LTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFA
VIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPAL
VNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYA
LRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDV
QTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQEL
RGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAK
GSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020857
ORF Size 2919 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020857.3
RefSeq Size 3922 bp
RefSeq ORF 2922 bp
Locus ID 57617
UniProt ID Q9P253, A0A024R9R3
Cytogenetics 15q15.1
Domains Pep3_Vps18
MW 110 kDa
Summary Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. provided by RefSeq, Jul 2008
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"NM_020857" in other vectors (6)
SKU Description Size Price
RC224536L1 Lenti ORF clone of Human vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18), Myc-DDK-tagged 10 ug
$1,191.00
RC224536L2 Lenti ORF clone of Human vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18), mGFP tagged 10 ug
$1,191.00
RC224536L3 Lenti ORF clone of Human vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18), Myc-DDK-tagged 10 ug
$1,191.00
RC224536L4 Lenti ORF clone of Human vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18), mGFP tagged 10 ug
$1,191.00
RG224536 VPS18 (tGFP-tagged) - Human vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18) 10 ug
$789.00 $1,091.00
SC110595 VPS18 (untagged)-Human vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18) 10 ug
$892.00

Plasmid Map for RC224536

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224536
Circular map for RC224536

file-alt Citations

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