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CWH43 (NM_025087) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC224386
CWH43 (Myc-DDK-tagged)-Human cell wall biogenesis 43 C-terminal homolog (S. cerevisiae) (CWH43)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $640.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CWH43
Synonyms CWH43-C; PGAP2IP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224386 representing NM_025087
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTCGCTGTGGAGAGAAATCCTCTTGGAGTCGCTGCTGGGATGTGTTTCTTGGTCTCTCTACCATG
ACCTGGGACCGATGATCTATTACTTTCCTTTGCAAACACTAGAACTCACTGGGCTTGAAGGTTTTAGTAT
AGCATTTCTTTCTCCAATATTCCTAACAATTACTCCTTTCTGGAAATTGGTTAACAAGAAGTGGATGCTA
ACCCTGCTGAGGATAATCACTATTGGCAGCATAGCCTCCTTCCAGGCTCCAAATGCCAAACTTCGACTGA
TGGTTCTTGCGCTTGGGGTGTCTTCCTCACTGATAGTGCAAGCTGTGACTTGGTGGTCAGGAAGTCATTT
GCAAAGGTACCTCAGAATTTGGGGATTCATTTTAGGACAGATTGTTCTTGTTGTTCTACGCATATGGTAT
ACTTCACTAAACCCAATCTGGAGTTATCAGATGTCCAACAAAGTGATACTGACATTAAGTGCCATAGCCA
CACTTGATCGTATTGGCACAGATGGTGACTGCAGTAAACCTGAAGAAAAGAAGACTGGTGAGGTAGCCAC
GGGGATGGCCTCTAGACCCAACTGGCTGCTGGCAGGGGCTGCTTTTGGTAGCCTTGTGTTCCTCACCCAC
TGGGTTTTTGGAGAAGTCTCTCTTGTTTCCAGATGGGCAGTGAGTGGGCATCCACATCCAGGGCCAGATC
CTAACCCATTTGGAGGTGCAGTACTGCTGTGCTTGGCAAGTGGATTGATGCTTCCATCTTGTTTGTGGTT
TCGTGGTACTGGTTTGATCTGGTGGGTTACAGGAACAGCTTCAGCTGCGGGGCTCCTTTACCTGCACACA
TGGGCAGCTGCTGTGTCTGGCTGTGTCTTCGCCATCTTTACTGCATCCATGTGGCCCCAAACACTTGGAC
ACCTTATTAACTCAGGGACAAACCCTGGGAAAACCATGACCATTGCCATGATATTTTATCTTCTAGAAAT
ATTTTTCTGTGCCTGGTGCACAGCTTTTAAGTTTGTCCCAGGAGGTGTCTACGCTAGAGAAAGATCAGAT
GTGCTTTTGGGGACAATGATGTTAATTATCGGGCTGAATATGCTATTTGGTCCTAAGAAAAACCTTGACT
TGCTTCTTCAAACAAAAAACAGTTCTAAAGTGCTTTTCAGAAAGAGTGAAAAATACATGAAACTTTTTCT
GTGGCTGCTTGTTGGTGTGGGATTGTTGGGATTAGGACTACGGCATAAAGCCTATGAGAGAAAACTGGGC
AAAGTGGCACCAACCAAAGAGGTCTCTGCTGCCATCTGGCCTTTCAGGTTTGGATATGACAATGAAGGGT
GGTCTAGTCTAGAAAGATCAGCTCACCTGCTCAATGAAACAGGTGCAGATTTCATAACAATTTTGGAGAG
TGATGCTTCTAAGCCCTATATGGGGAACAATGACTTAACCATGTGGCTAGGGGAAAAGTTGGGTTTCTAT
ACAGACTTTGGTCCAAGCACAAGGTATCACACTTGGGGGATTATGGCTTTGTCAAGATACCCAATTGTGA
AATCTGAGCATCACCTTCTTCCGTCACCAGAGGGCGAGATCGCACCAGCCATCACATTGACCGTTAACAT
TTCGGGCAAGCTGGTGGATTTTGTCGTGACACACTTTGGGAACCACGAAGATGACCTCGACAGGAAACTG
CAGGCTATTGCTGTTTCAAAACTACTGAAAAGTAGCTCTAATCAAGTGATATTTCTGGGATATATCACTT
CAGCACCTGGCTCCAGAGATTATCTACAGCTCACTGAACATGGCAATGTGAAGGATATCGACAGCACTGA
TCATGACAGATGGTGTGAATACATTATGTATCGAGGGCTGATCAGGTTGGGTTATGCAAGAATCTCCCAT
GCTGAACTGAGTGATTCAGAAATTCAGATGGCAAAATTTAGGATCCCTGATGACCCCACTAATTATAGAG
ACAACCAGAAAGTGGTCATAGACCACAGAGAAGTTTCTGAGAAAATTCATTTTAATCCCAGATTTGGATC
CTACAAAGAAGGACACAATTATGAAAACAACCATCATTTTCATATGAATACTCCCAAATACTTTTTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224386 representing NM_025087
Red=Cloning site Green=Tags(s)

MPSLWREILLESLLGCVSWSLYHDLGPMIYYFPLQTLELTGLEGFSIAFLSPIFLTITPFWKLVNKKWML
TLLRIITIGSIASFQAPNAKLRLMVLALGVSSSLIVQAVTWWSGSHLQRYLRIWGFILGQIVLVVLRIWY
TSLNPIWSYQMSNKVILTLSAIATLDRIGTDGDCSKPEEKKTGEVATGMASRPNWLLAGAAFGSLVFLTH
WVFGEVSLVSRWAVSGHPHPGPDPNPFGGAVLLCLASGLMLPSCLWFRGTGLIWWVTGTASAAGLLYLHT
WAAAVSGCVFAIFTASMWPQTLGHLINSGTNPGKTMTIAMIFYLLEIFFCAWCTAFKFVPGGVYARERSD
VLLGTMMLIIGLNMLFGPKKNLDLLLQTKNSSKVLFRKSEKYMKLFLWLLVGVGLLGLGLRHKAYERKLG
KVAPTKEVSAAIWPFRFGYDNEGWSSLERSAHLLNETGADFITILESDASKPYMGNNDLTMWLGEKLGFY
TDFGPSTRYHTWGIMALSRYPIVKSEHHLLPSPEGEIAPAITLTVNISGKLVDFVVTHFGNHEDDLDRKL
QAIAVSKLLKSSSNQVIFLGYITSAPGSRDYLQLTEHGNVKDIDSTDHDRWCEYIMYRGLIRLGYARISH
AELSDSEIQMAKFRIPDDPTNYRDNQKVVIDHREVSEKIHFNPRFGSYKEGHNYENNHHFHMNTPKYFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025087
ORF Size 2097 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025087.3
RefSeq Size 2486 bp
RefSeq ORF 2100 bp
Locus ID 80157
UniProt ID Q9H720
Cytogenetics 4p11
Protein Families Transmembrane
MW 78.4 kDa
Summary Involved in lipid remodeling during GPI-anchor maturation.UniProtKB/Swiss-Prot Function
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"NM_025087" in other vectors (4)
SKU Description Size Price
RC224386L3 Lenti-ORF clone of CWH43 (Myc-DDK-tagged)-Human cell wall biogenesis 43 C-terminal homolog (S. cerevisiae) (CWH43) 10 ug
$940.00
RC224386L4 Lenti-ORF clone of CWH43 (mGFP-tagged)-Human cell wall biogenesis 43 C-terminal homolog (S. cerevisiae) (CWH43) 10 ug
$940.00
RG224386 CWH43 (tGFP-tagged) - Human cell wall biogenesis 43 C-terminal homolog (S. cerevisiae) (CWH43) 10 ug
$840.00
SC110680 CWH43 (untagged)-Human cell wall biogenesis 43 C-terminal homolog (S. cerevisiae) (CWH43) 10 ug
$641.00

Plasmid Map for RC224386

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224386
Circular map for RC224386

file-alt Citations

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