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C14orf181 (NM_207442) Human Tagged ORF Clone

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SKU
RC224309
C14orf181 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 181 (C14orf181)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C14orf181
Synonyms FLJ39779
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224309 representing NM_207442
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATACGTACACCCGCAGCGCCTCCTTCACTCCGAAGACTTTCACTTTCCCCCTTGCACAAAACCGAA
CATCCCACCCCACCACCACCTCCAAGCTAAAATACCTGGGCCGCCCCTCCCCACGAAGGGACGCGCTCCA
GGCGAATGGGGAGGGCCCGGAAGTCGGGCCTGGGACAGAGGAGCCGGCCCACCGCCTCCCGGTCGGAAGC
GGCAGTGCAGGTGAGGCGGCGGCCAGCCTGGGGACTGCGCGGGTAAGGAGCGGGAGGGCGGAATGCACCG
CGAAGTTGGCGGGGGACGGCCACGCACGCCTCGCCTCGGCCTTCCTGCACCGTAGGGCCGCCCTCCCGCC
ACCCCTGGGCACCTGTCGACCACGTCTGGGCGAGGGCGGGTCGGCTCCCGCCCTCGGGCCCTCACCGCGG
CGGGGACGCCGGGTCAGCGAAGGGTTAAGGGACTCGCTCCCTCCTCTCGGCTCCCTCACCCTTGGAAAGT
CCCCGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224309 representing NM_207442
Red=Cloning site Green=Tags(s)

MNTYTRSASFTPKTFTFPLAQNRTSHPTTTSKLKYLGRPSPRRDALQANGEGPEVGPGTEEPAHRLPVGS
GSAGEAAASLGTARVRSGRAECTAKLAGDGHARLASAFLHRRAALPPPLGTCRPRLGEGGSAPALGPSPR
RGRRVSEGLRDSLPPLGSLTLGKSPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207442
ORF Size 498 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207442.2, NP_997325.2
RefSeq Size 1727 bp
RefSeq ORF 500 bp
Locus ID 400223
Cytogenetics 14q24.1
MW 17.1 kDa
Summary DISCONTINUED: This record has been withdrawn by NCBI and HGNC. This record was defined by AK097098.1, which is not sufficient evidence to define a distinct locus.
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"NM_207442" in other vectors (4)
SKU Description Size Price
RC224309L3 Lenti ORF clone of Human chromosome 14 open reading frame 181 (C14orf181), Myc-DDK-tagged 10 ug
$450.00
RC224309L4 Lenti ORF clone of Human chromosome 14 open reading frame 181 (C14orf181), mGFP tagged 10 ug
$450.00
RG224309 C14orf181 (tGFP-tagged) - Human chromosome 14 open reading frame 181 (C14orf181) 10 ug
$350.00
SC308547 C14orf181 (untagged)-Human chromosome 14 open reading frame 181 (C14orf181) 10 ug
$330.00

Plasmid Map for RC224309

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224309
Circular map for RC224309

file-alt Citations

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