BRIP1 (NM_032043) Human Tagged ORF Clone
$1,087.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BRIP1 |
| Synonyms | BACH1; FANCJ; OF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_032043 |
| ORF Size | 3747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_032043.1, NP_114432.1 |
| RefSeq Size | 8166 bp |
| RefSeq ORF | 3750 bp |
| Locus ID | 83990 |
| UniProt ID | Q9BX63 |
| Cytogenetics | 17q23.2 |
| Protein Families | Druggable Genome |
| MW | 140.9 kDa |
| Summary | The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_032043" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC224085L1 | Lenti ORF clone of Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1), Myc-DDK-tagged | 10 ug |
$1,387.00
|
|
| RC224085L2 | Lenti ORF clone of Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1), mGFP tagged | 10 ug |
$1,387.00
|
|
| RC224085L3 | Lenti ORF clone of Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1), Myc-DDK-tagged | 10 ug |
$1,387.00
|
|
| RC224085L4 | Lenti ORF clone of Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1), mGFP tagged | 10 ug |
$1,387.00
|
|
| RG224085 | BRIP1 (tGFP-tagged) - Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1) | 10 ug |
$1,287.00
|
|
| SC305391 | BRIP1 (untagged)-Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1) | 10 ug |
$1,197.00
|
|
Citations
Identification of a BRCA1-mRNA Splicing Complex Required for Efficient DNA Repair and Maintenance of Genomic Stability
Savage, Gorski, Barros et al
Mol. Cell (2014)
Savage, Gorski, Barros et al
Mol. Cell (2014)
Cited by 180
AI Summary: OriGene products contributed Myc-DDK tagged ATRIP (RC223562), BACH1 (RC224085), and EXO1 (RC200547) plasmids, which were used for transfection experiments to study the components and function of the BRCA1-mRNA splicing complex in DNA repair and genomic stability.
Snippet: Flag-tagged BRCA1 construct Fl4-BRCA1 was a kind gift from Professor Richard Baer, Columbia University, New York, USA. Myc-DDK tagged ATRIP (RC223562), BACH1 (RC224085), and EXO1 (RC200547) plasmids were purchased from Origene. Plasmids were transfected with Genejuice (Merck) as per the manufacturer’s instructions
Showing 1-1 of 1 papers.
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