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LEC (CCL16) (NM_004590) Human Tagged ORF Clone

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SKU
RC224009
CCL16 (Myc-DDK-tagged)-Human chemokine (C-C motif) ligand 16 (CCL16)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "LEC or NM_004590"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LEC
Synonyms CKb12; HCC-4; ILINCK; LCC-1; LEC; LMC; Mtn-1; NCC-4; NCC4; SCYA16; SCYL4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224009 representing NM_004590
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTCTCCGAGGCTGCCCTGTCTCTCCTTGTCCTCATCCTTATCATTACTTCGGCTTCTCGCAGCC
AGCCAAAAGTTCCTGAGTGGGTGAACACCCCATCCACCTGCTGCCTGAAGTATTATGAGAAAGTGTTGCC
AAGGAGACTAGTGGTGGGATACAGAAAGGCCCTCAACTGTCACCTGCCAGCAATCATCTTCGTCACCAAG
AGGAACCGAGAAGTCTGCACCAACCCCAATGACGACTGGGTCCAAGAGTACATCAAGGATCCCAACCTAC
CTTTGCTGCCTACCAGGAACTTGTCCACGGTTAAAATTATTACAGCAAAGAATGGTCAACCCCAGCTCCT
CAACTCCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224009 representing NM_004590
Red=Cloning site Green=Tags(s)

MKVSEAALSLLVLILIITSASRSQPKVPEWVNTPSTCCLKYYEKVLPRRLVVGYRKALNCHLPAIIFVTK
RNREVCTNPNDDWVQEYIKDPNLPLLPTRNLSTVKIITAKNGQPQLLNSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004590
ORF Size 360 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004590.4
RefSeq Size 1497 bp
RefSeq ORF 363 bp
Locus ID 6360
UniProt ID O15467
Cytogenetics 17q12
Domains IL8
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Chemokine signaling pathway, Cytokine-cytokine receptor interaction
MW 13.6 kDa
Summary This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10. provided by RefSeq, Jul 2008
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"NM_004590" in other vectors (6)
SKU Description Size Price
RC224009L1 Lenti ORF clone of Human chemokine (C-C motif) ligand 16 (CCL16), Myc-DDK-tagged 10 ug
$450.00
RC224009L2 Lenti ORF clone of Human chemokine (C-C motif) ligand 16 (CCL16), mGFP tagged 10 ug
$450.00
RC224009L3 Lenti ORF clone of Human chemokine (C-C motif) ligand 16 (CCL16), Myc-DDK-tagged 10 ug
$450.00
RC224009L4 Lenti ORF clone of Human chemokine (C-C motif) ligand 16 (CCL16), mGFP tagged 10 ug
$450.00
RG224009 CCL16 (tGFP-tagged) - Human chemokine (C-C motif) ligand 16 (CCL16) 10 ug
$489.00
SC117298 CCL16 (untagged)-Human chemokine (C-C motif) ligand 16 (CCL16) 10 ug
$150.00

Plasmid Map for RC224009

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224009
Circular map for RC224009

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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