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CACHD1 (NM_020925) Human Tagged ORF Clone

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SKU
RC224006
CACHD1 (Myc-DDK-tagged)-Human cache domain containing 1 (CACHD1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,065.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "CACHD1 or NM_020925"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CACHD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224006 representing NM_020925
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGAGGCTGGCGGCCGAGGAGCTGGGGGTCGTCACCATGCAGCGGATATTCAACTCCTTTGTTTACA
CTGAGAAAATCTCAAATGGAGAAAGTGAAGTACAGCAGCTAGCCAAAAAAATCCGAGAGAAGTTCAACCG
TTACTTGGATGTGGTCAATCGGAACAAGCAAGTTGTAGAAGCATCCTATACGGCTCACCTAACCTCTCCC
CTAACTGCAATTCAAGACTGCTGTACTATCCCACCTTCCATGATGGAATTCGATGGGAACTTTAATACCA
ATGTGTCTAGAACAATTAGTTGTGATCGACTTTCTACTACTGTTAATAGCCGGGCCTTCAATCCAGGACG
AGACTTAAATTCAGTTCTTGCAGACAACCTGAAATCCAACCCTGGAATTAAGTGGCAATATTTCAGTTCA
GAAGAAGGAATTTTCACTGTTTTCCCAGCACACAAGTTCCGGTGTAAGGGCAGCTACGAACACCGCAGTA
GACCCATCTACGTCTCTACAGTCCGGCCGCAGTCAAAGCACATAGTAGTGATTCTGGACCACGGGGCTTC
AGTCACAGACACTCAGCTTCAGATTGCCAAGGACGCTGCTCAGGTCATCCTCAGCGCCATCGATGAACAT
GACAAGATTTCTGTGTTAACTGTGGCAGATACCGTCCGGACTTGCTCACTAGACCAGTGCTATAAGACCT
TCTTGTCTCCAGCCACCAGTGAGACAAAAAGGAAAATGTCCACCTTTGTTAGCAGCGTGAAGTCTTCAGA
CAGTCCTACCCAGCACGCAGTGGGATTCCAAAAGGCATTTCAGCTGATTCGAAGTACAAACAATAACACA
AAGTTCCAAGCAAATACAGACATGGTCATCATTTACCTGTCAGCTGGCATTACATCAAAGGACTCTTCGG
AAGAAGATAAAAAAGCGACTCTCCAAGTCATCAATGAAGAAAATAGCTTTCTAAACAACTCTGTAATGAT
TCTCACCTATGCCCTCATGAACGATGGGGTGACTGGTTTGAAAGAGCTGGCTTTTCTGAGGGATCTAGCT
GAACAGAATTCAGGGAAGTACGGTGTGCCAGACCGGATGGCCTTGCCTGTGATTAAGGGCAGCATGATGG
TGCTGAATCAGTTGAGCAACCTGGAGACCACAGTGGGCAGGTTCTACACAAACCTTCCCAACCGGATGAT
TGATGAAGCCGTCTTCAGCCTGCCCTTCTCTGATGAGATGGGAGATGGTTTGATAATGACTGTGAGTAAA
CCCTGTTATTTTGGAAACCTACTTCTGGGAATTGTAGGTGTGGACGTGAATCTGGCTTACATTCTTGAAG
ACGTGACGTATTACCAAGACTCTTTGGCTTCCTATACTTTTCTCATAGACGACAAAGGATATACACTTAT
GCACCCATCTCTTACCAGGCCATATTTATTGTCAGAGCCCCCACTTCATACTGACATCATACATTATGAA
AATATTCCAAAATTTGAATTAGTTCGGCAAAATATCCTAAGCCTCCCTCTGGGCAGCCAGATTATCGCAG
TCCCTGTGAACTCATCCCTGTCTTGGCACATAAACAAGCTGAGAGAAACTGGAAAGGAAGCCTACAATGT
TAGCTATGCCTGGAAGATGGTACAAGACACTTCCTTTATTCTGTGTATTGTGGTGATACAACCAGAAATA
CCTGTGAAACAACTGAAGAACCTCAACACTGTTCCCAGCAGCAAGCTGCTGTACCACCGGCTGGATCTCC
TTGGCCAGCCCAGTGCTTGCCTCCACTTCAAACAGCTGGCAACCCTAGAAAGTCCCACCATCATGCTGTC
TGCTGGCAGCTTTTCCTCCCCCTATGAGCACCTCAGCCAGCCAGAGACAAAGCGCATGGTAGAGCACTAC
ACCGCCTATCTCAGCGACAACACCCGCCTCATTGCTAACCCGGGCCTCAAATTCTCTGTCAGAAATGAAG
TAATGGCTACCAGCCACGTCACAGATGAATGGATGACACAAATGGAAATGAGTAGCCTGAACACTTACAT
TGTCCGCCGTTACATAGCAACACCCAATGGCGTCCTCAGAATTTATCCTGGTTCCCTCATGGACAAAGCA
TTTGATCCCACTAGGAGACAATGGTATCTCCATGCAGTAGCTAATCCAGGGTTGATTTCTTTGACTGGTC
CTTACTTAGATGTTGGAGGAGCTGGTTATGTTGTGACAATCAGTCACACAATTCATTCATCCAGTACACA
GCTGTCTTCTGGGCACACTGTGGCTGTGATGGGCATTGACTTCACACTCAGATACTTCTACAAAGTTCTG
ATGGACCTATTACCTGTCTGTAACCAAGATGGTGGCAACAAAATAAGGTGCTTCATAATGGAGGACAGGG
GTTATCTGGTGGCGCACCCGACTCTCATCGACCCCAAAGGACATGCACCTGTGGAGCAGCAGCACATCAC
CCACAAGGAGCCCCTGGTAGCAAATGATATCCTCAACCACCCCAACTTTGTAAAGAAAAACCTGTGCAAC
AGCTTCAGTGACAGAACGGTCCAGAGGTTTTATAAATTCAACACCAGCCTTGCGGGGGATTTGACGAACC
TTGTGCATGGCAGCCACTGTTCCAAATACAGATTAGCAAGGATCCCAGGAACCAACGCGTTTGTTGGCAT
TGTCAACGAAACCTGCGACTCTCTTGCCTTCTGTGCCTGCAGCATGGTGGACCGACTCTGTCTCAACTGT
CACCGAATGGAACAAAATGAATGTGAATGTCCTTGTGAGTGCCCTCTAGAGGTCAATGAGTGCACTGGCA
ACCTCACCAATGCAGAGAACCGAAACCCCAGCTGCGAGGTCCACCAGGAGCCGGTGACATACACAGCTAT
TGACCCTGGCCTGCAAGATGCTCTTCACCAGTGTGTCAACAGCAGGTGCAGTCAGAGGCTGGAAAGTGGG
GACTGTTTTGGGGTGCTGGATTGTGAATGGTGCATGGTGGACAGTGATGGAAAGACTCACCTGGACAAAC
CCTACTGTGCCCCCCAGAAAGAATGCTTCGGGGGGATTGTGGGAGCCAAAAGTCCCTACGTTGATGACAT
GGGAGCAATAGGTGATGAGGTGATCACATTAAACATGATTAAAAGCGCCCCTGTGGGTCCTGTGGCTGGA
GGGATCATGGGATGCATCATGGTCTTGGTCCTGGCGGTGTATGCCTACCGCCACCAGATTCATCGCCGGA
GCCATCAGCATATGTCTCCTCTTGCTGCCCAAGAAATGTCAGTGCGTATGTCCAACCTGGAGAATGACAG
AGATGAAAGGGACGACGACAGCCACGAAGACAGAGGCATCATCAGCAACACTCGGTTTATAGCTGCGGTC
ATCGAACGACATGCACACAGTCCAGAAAGAAGGCGCCGCTACTGGGGTCGATCAGGAACAGAAAGTGATC
ATGGTTACAGCACCATGAGCCCACAGGAGGACAGTGAAAATCCTCCATGCAACAATGACCCCTTGTCAGC
CGGGGTCGATGTGGGAAACCATGATGAGGACTTAGACCTGGATACCCCCCCTCAGACTGCTGCCCTACTA
AGTCACAAGTTCCACCACTACCGGTCACACCACCCTACACTTCATCATAGCCACCACTTACAGGCGGCCG
TCACGGTACACACTGTCGATGCAGAATGC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224006 representing NM_020925
Red=Cloning site Green=Tags(s)

MRRLAAEELGVVTMQRIFNSFVYTEKISNGESEVQQLAKKIREKFNRYLDVVNRNKQVVEASYTAHLTSP
LTAIQDCCTIPPSMMEFDGNFNTNVSRTISCDRLSTTVNSRAFNPGRDLNSVLADNLKSNPGIKWQYFSS
EEGIFTVFPAHKFRCKGSYEHRSRPIYVSTVRPQSKHIVVILDHGASVTDTQLQIAKDAAQVILSAIDEH
DKISVLTVADTVRTCSLDQCYKTFLSPATSETKRKMSTFVSSVKSSDSPTQHAVGFQKAFQLIRSTNNNT
KFQANTDMVIIYLSAGITSKDSSEEDKKATLQVINEENSFLNNSVMILTYALMNDGVTGLKELAFLRDLA
EQNSGKYGVPDRMALPVIKGSMMVLNQLSNLETTVGRFYTNLPNRMIDEAVFSLPFSDEMGDGLIMTVSK
PCYFGNLLLGIVGVDVNLAYILEDVTYYQDSLASYTFLIDDKGYTLMHPSLTRPYLLSEPPLHTDIIHYE
NIPKFELVRQNILSLPLGSQIIAVPVNSSLSWHINKLRETGKEAYNVSYAWKMVQDTSFILCIVVIQPEI
PVKQLKNLNTVPSSKLLYHRLDLLGQPSACLHFKQLATLESPTIMLSAGSFSSPYEHLSQPETKRMVEHY
TAYLSDNTRLIANPGLKFSVRNEVMATSHVTDEWMTQMEMSSLNTYIVRRYIATPNGVLRIYPGSLMDKA
FDPTRRQWYLHAVANPGLISLTGPYLDVGGAGYVVTISHTIHSSSTQLSSGHTVAVMGIDFTLRYFYKVL
MDLLPVCNQDGGNKIRCFIMEDRGYLVAHPTLIDPKGHAPVEQQHITHKEPLVANDILNHPNFVKKNLCN
SFSDRTVQRFYKFNTSLAGDLTNLVHGSHCSKYRLARIPGTNAFVGIVNETCDSLAFCACSMVDRLCLNC
HRMEQNECECPCECPLEVNECTGNLTNAENRNPSCEVHQEPVTYTAIDPGLQDALHQCVNSRCSQRLESG
DCFGVLDCEWCMVDSDGKTHLDKPYCAPQKECFGGIVGAKSPYVDDMGAIGDEVITLNMIKSAPVGPVAG
GIMGCIMVLVLAVYAYRHQIHRRSHQHMSPLAAQEMSVRMSNLENDRDERDDDSHEDRGIISNTRFIAAV
IERHAHSPERRRRYWGRSGTESDHGYSTMSPQEDSENPPCNNDPLSAGVDVGNHDEDLDLDTPPQTAALL
SHKFHHYRSHHPTLHHSHHLQAAVTVHTVDAEC

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_020925
ORF Size 3669 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020925.2, NP_065976.2
RefSeq Size 5274 bp
RefSeq ORF 3825 bp
Locus ID 57685
UniProt ID Q5VU97, A0A0A0MQY7
Cytogenetics 1p31.3
Protein Families Druggable Genome, Transmembrane
MW 136.5 kDa
Summary May regulate voltage-dependent calcium channels.UniProtKB/Swiss-Prot Function
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Documents
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"NM_020925" in other vectors (7)
SKU Description Size Price
RC224006L1 Lenti ORF clone of Human cache domain containing 1 (CACHD1), Myc-DDK-tagged 10 ug
$1,365.00
RC224006L2 Lenti ORF clone of Human cache domain containing 1 (CACHD1), mGFP tagged 10 ug
$1,365.00
RC224006L3 Lenti ORF clone of Human cache domain containing 1 (CACHD1), Myc-DDK-tagged 10 ug
$1,365.00
RC224006L4 Lenti ORF clone of Human cache domain containing 1 (CACHD1), mGFP tagged 10 ug
$1,365.00
RG224006 CACHD1 (tGFP-tagged) - Human cache domain containing 1 (CACHD1) 10 ug
$1,265.00
SC315024 CACHD1 (untagged)-Human cache domain containing 1 (CACHD1) 10 ug
$1,172.00
SC318558 CACHD1 (untagged)-Human cache domain containing 1 (CACHD1) 10 ug
$1,065.00

Plasmid Map for RC224006

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224006
Circular map for RC224006

file-alt Citations

CACHD1 is an α2δ-like protein that modulates CaV3 voltage-gated calcium channel activity
Cottrell, Soubrane, Hounshell et al
J. Neurosci. (2018)
AI Summary: OriGene contributed to this study by providing the human CACHD1 construct, which was used to investigate the protein's role in modulating CaV3 voltage-gated calcium channel activity.
Snippet: used for Fig. 4A. 153 154 Vectors and vector construction 155 The human CACHD1 construct was purchased from Origene (Rockville, MA, USA) and the 156 truncated clone completed by PCR. The subsequent open reading frame was then subcloned 157 into pcDNA5
Showing 1-1 of 1 papers.
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