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SPACA5 (NM_205856) Human Tagged ORF Clone

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SKU
RC224004
SPACA5 (Myc-DDK-tagged)-Human sperm acrosome associated 5 (SPACA5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPACA5
Synonyms LYC5; LYZL5; PNPK6288; SLLP-X; SLLP2; UNQ6288
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224004 representing NM_205856
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGCCTGGGGCACTGTGGTAGTGACCTTGGCCACGCTGATGGTTGTCACTGTGGATGCCAAGATCT
ATGAACGCTGCGAGCTGGCGGCAAGACTGGAGAGAGCAGGGCTGAACGGCTACAAGGGCTACGGCGTTGG
AGACTGGCTGTGCATGGCTCATTATGAGAGTGGCTTTGACACCGCCTTCGTGGACCACAATCCTGATGGC
AGCAGTGAATATGGCATTTTCCAACTGAATTCTGCCTGGTGGTGTGACAATGGCATTACACCCACCAAGA
ACCTCTGCCACATGGATTGTCATGACCTGCTCAATCGCCATATTCTGGATGACATCAGGTGTGCCAAGCA
GATTGTGTCCTCACAGAATGGGCTTTCTGCCTGGACTTCTTGGAGGCTACACTGTTCTGGCCATGATTTA
TCTGAATGGCTCAAGGGGTGTGATATGCATGTGAAAATTGATCCAAAAATTCATCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224004 representing NM_205856
Red=Cloning site Green=Tags(s)

MKAWGTVVVTLATLMVVTVDAKIYERCELAARLERAGLNGYKGYGVGDWLCMAHYESGFDTAFVDHNPDG
SSEYGIFQLNSAWWCDNGITPTKNLCHMDCHDLLNRHILDDIRCAKQIVSSQNGLSAWTSWRLHCSGHDL
SEWLKGCDMHVKIDPKIHP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_205856
ORF Size 477 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_205856.3, NP_995328.2
RefSeq Size 732 bp
RefSeq ORF 480 bp
Locus ID 389852
UniProt ID Q96QH8, A0A140VJN7
Cytogenetics Xp11.23
MW 17.7 kDa
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Documents
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"NM_205856" in other vectors (4)
SKU Description Size Price
RC224004L3 Lenti ORF clone of Human sperm acrosome associated 5 (SPACA5), Myc-DDK-tagged 10 ug
$450.00
RC224004L4 Lenti ORF clone of Human sperm acrosome associated 5 (SPACA5), mGFP tagged 10 ug
$450.00
RG224004 SPACA5 (tGFP-tagged) - Human sperm acrosome associated 5 (SPACA5) 10 ug
$350.00
SC308244 SPACA5 (untagged)-Human sperm acrosome associated 5 (SPACA5) 10 ug
$165.00

Plasmid Map for RC224004

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224004
Circular map for RC224004

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