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CTDSPL (NM_001008392) Human Tagged ORF Clone

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SKU
RC223937
CTDSPL (Myc-DDK-tagged)-Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CTDSPL
Synonyms C3orf8; HYA22; PSR1; RBSP3; SCP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223937 representing NM_001008392
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGGCCCGGCCATCATCACCCAGGTGACCAACCCCAAGGAGGACGAGGGCCGGTTGCCGGGCGCGG
GCGAGAAAGCCTCCCAGTGCAACGTCAGCTTAAAGAAGCAGAGGAGCCGCAGCATCCTTAGCTCCTTCTT
CTGCTGCTTCCGTGATTACAATGTGGAGGCCCCTCCACCCAGCAGCCCCAGTGTGCTTCCGCCACTGGTG
GAGGAGAATGGTGGGCTTCAGAAGGGTGACCAGAGGCAGGTCATTCCCATACCAAGTCCACCAGCTAAGT
ACCTTCTTCCAGAGGTGACGGTGCTTGACTATGGAAAGAAATGTGTGGTCATTGATTTAGATGAAACATT
GGTGCACAGTTCGTTTAAGCCTATTAGTAATGCTGATTTTATTGTTCCGGTTGAAATCGATGGAACTATA
CATCAGGTGTATGTGCTGAAGCGGCCACATGTGGACGAGTTCCTCCAGAGGATGGGGCAGCTTTTTGAAT
GTGTGCTCTTTACTGCCAGCTTGGCCAAGTATGCAGACCCTGTGGCTGACCTCCTAGACCGCTGGGGTGT
GTTCCGGGCCCGGCTCTTCAGAGAATCATGTGTTTTTCATCGTGGGAACTACGTGAAGGACCTGAGTCGC
CTTGGGCGGGAGCTGAGCAAAGTGATCATTGTTGACAATTCCCCTGCCTCATACATCTTCCATCCTGAGA
ATGCAGTGCCTGTGCAGTCCTGGTTCGATGACATGACGGACACGGAGCTGCTGGACCTCATCCCCTTCTT
TGAGGGCCTGAGCCGGGAGGACGACGTGTACAGCATGCTGCACAGACTCTGCAATAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223937 representing NM_001008392
Red=Cloning site Green=Tags(s)

MDGPAIITQVTNPKEDEGRLPGAGEKASQCNVSLKKQRSRSILSSFFCCFRDYNVEAPPPSSPSVLPPLV
EENGGLQKGDQRQVIPIPSPPAKYLLPEVTVLDYGKKCVVIDLDETLVHSSFKPISNADFIVPVEIDGTI
HQVYVLKRPHVDEFLQRMGQLFECVLFTASLAKYADPVADLLDRWGVFRARLFRESCVFHRGNYVKDLSR
LGRELSKVIIVDNSPASYIFHPENAVPVQSWFDDMTDTELLDLIPFFEGLSREDDVYSMLHRLCNR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001008392
ORF Size 828 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001008392.2
RefSeq Size 4455 bp
RefSeq ORF 831 bp
Locus ID 10217
UniProt ID O15194
Cytogenetics 3p22.2
Protein Families Druggable Genome, Phosphatase
MW 30.9 kDa
Summary Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001008392" in other vectors (4)
SKU Description Size Price
RC223937L3 Lenti ORF clone of Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC223937L4 Lenti ORF clone of Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 1, mGFP tagged 10 ug
$600.00
RG223937 CTDSPL (tGFP-tagged) - Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 1 10 ug
$489.00 $500.00
SC128019 CTDSPL (untagged)-Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 1 10 ug
$300.00

Plasmid Map for RC223937

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223937
Circular map for RC223937

file-alt Citations

Control of PD-L1 expression by miR-140/142/340/383 and oncogenic activation of the OCT4-miR-18a pathway in cervical cancer
Dong, Xiong, Yu et al
Oncogene (2018)
AI Summary: In this study, OriGene products—specifically the human PD-L1 shRNA plasmid (OriGene, #TF314508) and PD-L1 overexpression plasmid (OriGene, #RC213307)—were used to modulate PD-L1 expression in cervical cancer cells, enabling the authors to investigate the functional role and regulatory mechanisms of PD-L1 in cancer progression.
Showing 1-1 of 1 papers.
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