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Glycoprotein VI (GP6) (NM_016363) Human Tagged ORF Clone

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SKU
RC223897
GP6 (Myc-DDK-tagged)-Human glycoprotein VI (platelet) (GP6), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Glycoprotein VI or NM_016363"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Glycoprotein VI
Synonyms BDPLT11; GPIV; GPVI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223897 representing NM_016363
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCCATCCCCGACCGCCCTCTTCTGTCTTGGGCTGTGTCTGGGGCGTGTGCCAGCGCAGAGTGGAC
CGCTCCCCAAGCCCTCCCTCCAGGCTCTGCCCAGCTCCCTGGTGCCCCTGGAGAAGCCAGTGACCCTCCG
GTGCCAGGGACCTCCGGGCGTGGACCTGTACCGCCTGGAGAAGCTGAGTTCCAGCAGGTACCAGGATCAG
GCAGTCCTCTTCATCCCGGCCATGAAGAGAAGTCTGGCTGGACGCTACCGCTGCTCCTACCAGAACGGAA
GCCTCTGGTCCCTGCCCAGCGACCAGCTGGAGCTCGTTGCCACGGGAGTTTTTGCCAAACCCTCGCTCTC
AGCCCAGCCCGGCCCGGCGGTGTCGTCAGGAGGGGACGTAACCCTACAGTGTCAGACTCGGTATGGCTTT
GACCAATTTGCTCTGTACAAGGAAGGGGACCCTGCGCCCTACAAGAATCCCGAGAGATGGTACCGGGCTA
GTTTCCCCATCATCACGGTGACCGCCGCCCACAGCGGAACCTACCGATGCTACAGCTTCTCCAGCAGGGA
CCCATACCTGTGGTCGGCCCCCAGCGACCCCCTGGAGCTTGTGGTCACAGGAACCTCTGTGACCCCCAGC
CGGTTACCAACAGAACCACCTTCCTCGGTAGCAGAATTCTCAGAAGCCACCGCTGAACTGACCGTCTCAT
TCACAAACAAAGTCTTCACAACTGAGACTTCTAGGAGTATCACCACCAGTCCAAAGGAGTCAGACTCTCC
AGCTGGTCCTGCCCGCCAGTACTACACCAAGGGCAACCTGGTCCGGATATGCCTCGGGGCTGTGATCCTA
ATAATCCTGGCGGGGTTTCTGGCAGAGGACTGGCACAGCCGGAGGAAGCGCCTGCGGCACAGGGGCAGGG
CTGTGCAGAGGCCGCTTCCGCCCCTGCCGCCCCTCCCGCAGACCCGGAAATCACACGGGGGTCAGGATGG
AGGCCGACAGGATGTTCACAGCCGCGGGTTATGTTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223897 representing NM_016363
Red=Cloning site Green=Tags(s)

MSPSPTALFCLGLCLGRVPAQSGPLPKPSLQALPSSLVPLEKPVTLRCQGPPGVDLYRLEKLSSSRYQDQ
AVLFIPAMKRSLAGRYRCSYQNGSLWSLPSDQLELVATGVFAKPSLSAQPGPAVSSGGDVTLQCQTRYGF
DQFALYKEGDPAPYKNPERWYRASFPIITVTAAHSGTYRCYSFSSRDPYLWSAPSDPLELVVTGTSVTPS
RLPTEPPSSVAEFSEATAELTVSFTNKVFTTETSRSITTSPKESDSPAGPARQYYTKGNLVRICLGAVIL
IILAGFLAEDWHSRRKRLRHRGRAVQRPLPPLPPLPQTRKSHGGQDGGRQDVHSRGLCS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016363
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016363.1
RefSeq Size 2263 bp
RefSeq ORF 1020 bp
Locus ID 51206
UniProt ID Q9HCN6
Cytogenetics 19q13.42
Protein Families Druggable Genome, Transmembrane
Protein Pathways ECM-receptor interaction
MW 36.7 kDa
Summary This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Dec 2011
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"NM_016363" in other vectors (6)
SKU Description Size Price
RC223897L1 Lenti ORF clone of Human glycoprotein VI (platelet) (GP6), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC223897L2 Lenti ORF clone of Human glycoprotein VI (platelet) (GP6), transcript variant 2, mGFP tagged 10 ug
$757.00
RC223897L3 Lenti ORF clone of Human glycoprotein VI (platelet) (GP6), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC223897L4 Lenti ORF clone of Human glycoprotein VI (platelet) (GP6), transcript variant 2, mGFP tagged 10 ug
$757.00
RG223897 GP6 (tGFP-tagged) - Human glycoprotein VI (platelet) (GP6), transcript variant 2 10 ug
$489.00 $657.00
SC304410 GP6 (untagged)-Human glycoprotein VI (platelet) (GP6), transcript variant 2 10 ug
$457.00

Plasmid Map for RC223897

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223897
Circular map for RC223897

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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