FADS2 (NM_004265) Human Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | FADS2 |
| Synonyms | D6D; DES6; FADSD6; LLCDL2; SLL0262; TU13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004265 |
| ORF Size | 1332 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004265.4 |
| RefSeq Size | 3149 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 9415 |
| UniProt ID | O95864 |
| Cytogenetics | 11q12.2 |
| Domains | FA_desaturase, heme_1 |
| Protein Families | Transmembrane |
| Protein Pathways | alpha-Linolenic acid metabolism, Biosynthesis of unsaturated fatty acids, PPAR signaling pathway |
| MW | 52.1 kDa |
| Summary | The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004265" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223780L1 | Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC223780L2 | Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), mGFP tagged | 10 ug |
$986.00
|
|
| RC223780L3 | Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC223780L4 | Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), mGFP tagged | 10 ug |
$986.00
|
|
| RG223780 | FADS2 (tGFP-tagged) - Human fatty acid desaturase 2 (FADS2) | 10 ug |
$886.00
|
|
| SC117468 | FADS2 (untagged)-Human fatty acid desaturase 2 (FADS2) | 10 ug |
$686.00
|
|
Citations
Polyunsaturated Fatty Acid Desaturation Is a Mechanism for Glycolytic NAD+ Recycling
Kim, Deik, Gonzalez et al
Cell Metab. (2019) 29 (4), 856-870.e7 DOI: 10.1016/j.cmet.2018.12.023
Kim, Deik, Gonzalez et al
Cell Metab. (2019) 29 (4), 856-870.e7 DOI: 10.1016/j.cmet.2018.12.023
AI Summary: OriGene products, specifically the pCMV6-Entry-empty vector (Cat#PS100001), pCMV6-Entry-human FADS1 (Cat#RC229252), and pCMV6-Entry-human FADS2 (Cat#RC223780), were used in this study to manipulate expression of desaturase enzymes and investigate their role in NAD+ recycling during glycolysis.
Snippet: pCMV6-Entry-empty OriGene Technologies Cat#PS100001 pCMV6-Entry-human FADS1 OriGene Technologies Cat#RC229252 pCMV6-Entry-human FADS2 OriGene Technologies Cat#RC223780 pRS-scrambled non-effective shRNA control
Showing 1-1 of 1 papers.
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