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EMR2 (ADGRE2) (NM_152918) Human Tagged ORF Clone

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SKU
RC223677
EMR2 (Myc-DDK-tagged)-Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $624.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EMR2
Synonyms CD312; EMR2; VBU
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223677 representing NM_152918
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCC
AGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTG
CAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAG
TGTGCAACACTGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTACGACT
GCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACGTG
TCAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTC
GACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCCAATAACACCATCCAGAGCATCTTAC
AGGCGCTGGATGAGCTGCTGGAGGCCCCTGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCACTGTGT
GGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGCAAGAACCTTTCCAATGGGCTG
TTGAACTTCAGTTATCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAGAAGCAAGTAGACAGGAGTGTCA
CCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCCAGGCCC
TTCTGTGGTGGGCCTTGTCTCCATTCCAGGGATGGGCAAGTTGCTGGCTGAGGCCCCTCTGGTCCTGGAA
CCTGAGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCCCCCATCCTGCTCT
CAGATGTGATCTCTGCCTTTCTGAGCAACAACGACACCCAAAACCTCAGCTCCCCAGTTACCTTCACCTT
CTCCCACCGTTCAGTGATCCCGAGACAGAAGGTGCTCTGTGTCTTCTGGGAGCATGGCCAGAATGGATGT
GGTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACACCAGCACCATCTGCCGTTGCACCC
ACCTGAGCAGCTTTGCCGTCCTCATGGCCCACTACGATGTGCAGGAGGAGGATCCCGTGCTGACTGTCAT
CACCTACATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTGTGTAAA
GCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACCTCCTCT
TCCTCGTGGCAATTGATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCACTATCT
CTACCTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACCTCTTCCTCACTGCACGGAACCTGACG
GTGGTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAGTCCCAG
CTGTGACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTGGCTCCA
ACCAGAAAAGGGATTTATATGGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCTGTGAATTTAGTTCTC
TTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCCTCCGGA
ACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCTGGGCAT
CTTGCAGGTGGGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAGGGTGTC
TTCATCTTCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGGAAATGGTCCAAAGGGA
TCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGACACCTCCAAACC
CAGCACGGTTAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223677 representing NM_152918
Red=Cloning site Green=Tags(s)

MGGRVFLVFLAFCVWLTLPGAETQDSRGCARWCPQDSSCVNATACRCNPGFSSFSEIITTPMETCDDINE
CATLSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDMTFSTWTPPPGVHSQTLSRFF
DKVQDLGRDYKPGLANNTIQSILQALDELLEAPGDLETLPRLQQHCVASHLLDGLEDVLRGLSKNLSNGL
LNFSYPAGTELSLEVQKQVDRSVTLRQNQAVMQLDWNQAQKSGDPGPSVVGLVSIPGMGKLLAEAPLVLE
PEKQMLLHETHQGLLQDGSPILLSDVISAFLSNNDTQNLSSPVTFTFSHRSVIPRQKVLCVFWEHGQNGC
GHWATTGCSTIGTRDTSTICRCTHLSSFAVLMAHYDVQEEDPVLTVITYMGLSVSLLCLLLAALTFLLCK
AIQNTSTSLHLQLSLCLFLAHLLFLVAIDQTGHKVLCSIIAGTLHYLYLATLTWMLLEALYLFLTARNLT
VVNYSSINRFMKKLMFPVGYGVPAVTVAISAASRPHLYGTPSRCWLQPEKGFIWGFLGPVCAIFSVNLVL
FLVTLWILKNRLSSLNSEVSTLRNTRMLAFKATAQLFILGCTWCLGILQVGPAARVMAYLFTIINSLQGV
FIFLVYCLLSQQVREQYGKWSKGIRKLKTESEMHTLSSSAKADTSKPSTVN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152918
ORF Size 2043 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152918.1, NP_690882.1
RefSeq Size 6345 bp
RefSeq ORF 2045 bp
Locus ID 30817
Cytogenetics 19p13.12
Protein Families Druggable Genome, GPCR, Transmembrane
MW 74.8 kDa
Summary This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Aug 2012
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"NM_152918" in other vectors (4)
SKU Description Size Price
RC223677L3 Lenti-ORF clone of EMR2 (Myc-DDK-tagged)-Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 4 10 ug
$924.00
RC223677L4 Lenti-ORF clone of EMR2 (mGFP-tagged)-Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 4 10 ug
$924.00
RG223677 EMR2 (tGFP-tagged) - Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 4 10 ug
$824.00
SC306522 EMR2 (untagged)-Human egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 4 10 ug
$687.00

Plasmid Map for RC223677

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223677
Circular map for RC223677

file-alt Citations

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