Sperm Flagellar 2 (SPEF2) (NM_144722) Human Tagged ORF Clone
$479.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Sperm Flagellar 2 |
| Synonyms | CT122; KPL2; SPGF43 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_144722 |
| ORF Size | 1542 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_144722.4 |
| RefSeq Size | 3000 bp |
| RefSeq ORF | 1545 bp |
| Locus ID | 79925 |
| UniProt ID | Q9C093 |
| Cytogenetics | 5p13.2 |
| MW | 61 kDa |
| Summary | Required for correct axoneme development in spermatozoa. Important for normal development of the manchette and sperm head morphology. Essential for male fertility. Plays a role in localization of the intraflagellar transport protein IFT20 to the manchette, suggesting function as an adapter for dynein-mediated protein transport during spermatogenesis. Also plays a role in bone growth where it seems to be required for normal osteoblast differentiation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_144722" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223626L3 | Lenti ORF clone of Human sperm flagellar 2 (SPEF2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$779.00
|
|
| RC223626L4 | Lenti ORF clone of Human sperm flagellar 2 (SPEF2), transcript variant 2, mGFP tagged | 10 ug |
$779.00
|
|
| RG223626 | SPEF2 (tGFP-tagged) - Human sperm flagellar 2 (SPEF2), transcript variant 2 | 10 ug |
$679.00
|
|
| SC313797 | SPEF2 (untagged)-Human sperm flagellar 2 (SPEF2), transcript variant 2 | 10 ug |
$527.00
|
|
Citations
A dominant negative splice variant of the heparan sulfate biosynthesis enzyme NDST1 reduces heparan sulfate sulfation
Missaghian, Dierker, Khosrowabadi et al
Glycobiology (2022) 32 (6), 518-528 DOI: 10.1093/glycob/cwac004
Missaghian, Dierker, Khosrowabadi et al
Glycobiology (2022) 32 (6), 518-528 DOI: 10.1093/glycob/cwac004
Cited by 13
AI Summary: OriGene’s pCMV6 vector containing human NDST1 cDNA (RC221638) with a C-terminal Myc/Flag-tag was used to express both the full-length (NDST1A) and splice variant (NDST1B) forms of NDST1 in HEK293 and HeLa cells, enabling the study of their roles in heparan sulfate sulfation.
Snippet: rials and methods cDNA constructs For transfection of HEK 293 and HeLa cells A pCMV6-vector containing cDNA (RC221638) encoding human NDST1(NM__01543) with a C-terminal Myc/Flag-tag was obtained from Origene. The corresponding protein product is in this report denoted NDST1A. This vector was also used to generate the shorter form of NDST1, here denoted NDST1B, through a Quick Change mutagenesis ap;- A pCMV6-vector containing cDNA (RC221638) encoding human NDST1(NM__01543) with a C-terminal Myc/Flag-tag was obtained from Origene. …
Generation of a Polyclonal Antibody against the Mouse Metal Transporter ZIP8
Wei, Wu, Zhao
Antibodies (Basel) (2021) 10 (2) DOI: 10.3390/antib10020016
Wei, Wu, Zhao
Antibodies (Basel) (2021) 10 (2) DOI: 10.3390/antib10020016
Cited by 5
Snippet: 2.2. Plasmid Construction. The sequences encoding serine 30 to serine 124 of mZIP8 were amplified by polymerase chain reaction (PCR) using pCMV-Entry-mZIP8 expression vector (Origene, Rockville, MD, USA) as the template Cached;- 2.2. Plasmid Construction The sequences encoding serine 30 to serine 124 of mZIP8 were amplified by polymer- ase chain reaction (PCR) using pCMV-Entry-mZIP8 expr...
ECT2 promotes lung adenocarcinoma progression through extracellular matrix dynamics and focal adhesion signaling
Kosibaty, Murata, Minami et al
Cancer Sci (2021) 112 (2), 703-714 DOI: 10.1111/cas.14743
Kosibaty, Murata, Minami et al
Cancer Sci (2021) 112 (2), 703-714 DOI: 10.1111/cas.14743
Cited by 31
AI Summary: In this study, OriGene products including ECT2 shRNA lentiviral particles (TL309370V) and ECT2 cDNA clone (RC206434) were used to manipulate ECT2 expression in lung adenocarcinoma cells, enabling the authors to investigate ECT2's role in extracellular matrix dynamics and focal adhesion signaling.
Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins
Wu, Lu, Feng et al
Cells (2020) 9 (4) DOI: 10.3390/cells9041027
Wu, Lu, Feng et al
Cells (2020) 9 (4) DOI: 10.3390/cells9041027
Cited by 41
AI Summary: In this study, the Flag-tagged human matriptase expression plasmid from OriGene was used to investigate the role of matriptase in cleaving EpCAM and TROP2 in keratinocytes, thereby elucidating its impact on the stability of these proteins and associated claudins.
Snippet: 2.2. Gene Expression Plasmids. pcDNA3-HAEpCAM has been described [22]. Plasmid expressing Flag-tagged human matriptase was obtained from OriGene (Rockville, MD, USA). PCR-amplified HA-tagged mouse TROP2 cDNA was cloned into pcDNA3 Cached;- 2.2. Gene Expression Plasmids pcDNA3-HAEpCAM has been described [22]. Plasmid expressing Flag-tagged human matriptase was obtained from OriGene (Rockv...
Transcriptomic Alterations in Lung Adenocarcinoma Unveil New Mechanisms Targeted by the TBX2 Subfamily of Tumor Suppressor Genes
Khalil, Dekmak, Boulos et al
Front Oncol (2018) 8, 482 DOI: 10.3389/fonc.2018.00482
Khalil, Dekmak, Boulos et al
Front Oncol (2018) 8, 482 DOI: 10.3389/fonc.2018.00482
AI Summary: OriGene products, specifically the human Flag tagged pORF-TBX2 and pORF-TBX4 expression plasmids, were used to overexpress TBX2 subfamily genes in lung cancer cell lines, enabling the study of their tumor suppressor functions in lung adenocarcinoma.
Snippet: …Incubation of cells was carried out in a 5% CO2 humid atmosphere at 37°C. Mouse HA tagged pCGN-Tbx5 and human HA tagged pCGN-TBX3 were previously described while human Flag tagged pORF-TBX (2 and 4) were purchased from Origene
Aldosterone induces inflammatory cytokines in penile corpus cavernosum by activating the NF-κB pathway
Wu, Xiong, Mao et al
Asian J. Androl. (2017)
Wu, Xiong, Mao et al
Asian J. Androl. (2017)
AI Summary: OriGene’s Flag/Myc-tagged MRs plasmid was used in this study to achieve ectopic overexpression of mineralocorticoid receptors, enabling the authors to demonstrate that increased MRs expression enhances aldosterone-induced activation of the NF-κB pathway in penile corpus cavernosum tissue.
Snippet: In selected experiments, cells were co-incubated with spironolactone. Ectopic overexpression and endogenous knockdown A Flag/Myc-tagged MRs plasmid was purchased from OriGene Technologies (Rockville, MD, USA). ... All 3 versions Cite SaveSaving
Showing 1-6 of 9 papers.
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