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ATP6V1C2 (NM_001039362) Human Tagged ORF Clone

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SKU
RC223434
ATP6V1C2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 (ATP6V1C2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ATP6V1C2 or NM_001039362"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ATP6V1C2
Synonyms ATP6C2; VMA5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223434 representing NM_001039362
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGAGTTTTGGTTAATTTCTGCCCCTGGCGATAAGGAAAATTTGCAAGCTCTGGAGAGGATGAATA
CTGTAACCTCCAAGTCCAACCTGTCTTATAATACCAAATTCGCTATTCCTGACTTCAAGGTGGGGACCTT
GGATTCCCTGGTTGGCCTCTCTGATGAGTTGGGGAAACTCGACACCTTTGCTGAAAGCCTCATAAGGAGA
ATGGCTCAGAGCGTGGTGGAAGTCATGGAGGACTCAAAGGGGAAGGTCCAGGAGCACCTCCTGGCAAACG
GAGTTGACTTAACATCCTTTGTGACCCACTTTGAATGGGACATGGCCAAATATCCTGTCAAGCAGCCGCT
CGTGAGTGTGGTGGACACAATAGCCAAGCAACTGGCGCAGATCGAGATGGACCTGAAGTCCCGAACGGCC
GCCTACAACACTCTGAAGACAAACCTGGAGAACCTGGAAAAGAAATCCATGGGGAACCTCTTCACCCGGA
CACTGAGTGATATTGTGAGCAAAGAGGACTTCGTGCTGGATTCTGAATATCTCGTCACACTTCTGGTCAT
CGTCCCCAAACCAAACTACTCACAATGGCAAAAAACCTACGAATCTCTCTCAGACATGGTGGTCCCTCGA
TCAACCAAACTCATTACTGAGGACAAGGAAGGGGGCCTTTTCACTGTGACTCTGTTTCGAAAAGTGATTG
AAGATTTCAAAACCAAGGCCAAAGAAAACAAGTTCACTGTTCGTGAATTTTACTATGATGAGAAGGAAAT
TGAAAGGGAAAGGGAGGAGATGGCCAGATTGCTGTCTGATAAGAAGCAACAGTATCAAACTTCCTGTGTT
GCTCTTAAAAAGGGATCATCCACCTTCCCGGACCACAAGGTTAAGGTAACCCCGCTAGGTAACCCTGATA
GGCCTGCTGCGGGGCAGACCGACAGAGAGAGAGAGAGTGAGGGCGAGGGTGAGGGCCCCCTGCTGCGCTG
GCTCAAGGTGAACTTCAGTGAAGCCTTCATTGCCTGGATCCACATCAAGGCCCTGAGAGTGTTTGTGGAG
TCCGTGCTCAGGTATGGACTACCAGTGAACTTCCAGGCAGTGCTCCTGCAGCCGCATAAGAAGTCATCCA
CCAAGCGTTTAAGAGAGGTTCTAAACTCTGTCTTCCGACATCTGGATGAAGTAGCCGCTACAAGTATACT
GGATGCATCTGTGGAGATCCCGGGACTGCAACTCAATAACCAAGACTATTTTCCTTATGTCTACTTCCAT
ATTGACCTTAGTCTTCTTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223434 representing NM_001039362
Red=Cloning site Green=Tags(s)

MSEFWLISAPGDKENLQALERMNTVTSKSNLSYNTKFAIPDFKVGTLDSLVGLSDELGKLDTFAESLIRR
MAQSVVEVMEDSKGKVQEHLLANGVDLTSFVTHFEWDMAKYPVKQPLVSVVDTIAKQLAQIEMDLKSRTA
AYNTLKTNLENLEKKSMGNLFTRTLSDIVSKEDFVLDSEYLVTLLVIVPKPNYSQWQKTYESLSDMVVPR
STKLITEDKEGGLFTVTLFRKVIEDFKTKAKENKFTVREFYYDEKEIEREREEMARLLSDKKQQYQTSCV
ALKKGSSTFPDHKVKVTPLGNPDRPAAGQTDRERESEGEGEGPLLRWLKVNFSEAFIAWIHIKALRVFVE
SVLRYGLPVNFQAVLLQPHKKSSTKRLREVLNSVFRHLDEVAATSILDASVEIPGLQLNNQDYFPYVYFH
IDLSLLD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039362
ORF Size 1281 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039362.2
RefSeq Size 3250 bp
RefSeq ORF 1284 bp
Locus ID 245973
UniProt ID Q8NEY4
Cytogenetics 2p25.1
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
MW 48.6 kDa
Summary This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms. provided by RefSeq, Jul 2008
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Documents
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"NM_001039362" in other vectors (4)
SKU Description Size Price
RC223434L3 Lenti-ORF clone of ATP6V1C2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 (ATP6V1C2), transcript variant 1 10 ug
$757.00
RC223434L4 Lenti-ORF clone of ATP6V1C2 (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 (ATP6V1C2), transcript variant 1 10 ug
$757.00
RG223434 ATP6V1C2 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 (ATP6V1C2), transcript variant 1 10 ug
$657.00
SC310898 ATP6V1C2 (untagged)-Human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 (ATP6V1C2), transcript variant 1 10 ug
$503.00

Plasmid Map for RC223434

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223434
Circular map for RC223434

file-alt Citations

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