TLL1 (NM_012464) Human Tagged ORF Clone
$882.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TLL1 |
| Synonyms | ASD6; TLL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012464 |
| ORF Size | 3039 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012464.5 |
| RefSeq Size | 6654 bp |
| RefSeq ORF | 3042 bp |
| Locus ID | 7092 |
| UniProt ID | O43897, B7ZLW3 |
| Cytogenetics | 4q32.3 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 114.5 kDa |
| Summary | This gene encodes an astacin-like, zinc-dependent, metalloprotease that belongs to the peptidase M12A family. This protease processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Studies in mice suggest that this gene plays multiple roles in the development of mammalian heart, and is essential for the formation of the interventricular septum. Allelic variants of this gene are associated with atrial septal defect type 6. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012464" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223360L1 | Lenti ORF clone of Human tolloid-like 1 (TLL1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,182.00
|
|
| RC223360L2 | Lenti ORF clone of Human tolloid-like 1 (TLL1), transcript variant 1, mGFP tagged | 10 ug |
$1,182.00
|
|
| RC223360L3 | Lenti ORF clone of Human tolloid-like 1 (TLL1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,182.00
|
|
| RC223360L4 | Lenti ORF clone of Human tolloid-like 1 (TLL1), transcript variant 1, mGFP tagged | 10 ug |
$1,182.00
|
|
| RG223360 | TLL1 (tGFP-tagged) - Human tolloid-like 1 (TLL1), transcript variant 1 | 10 ug |
$1,082.00
|
|
| SC128122 | TLL1 (untagged)-Human tolloid-like 1 (TLL1), transcript variant 1 | 10 ug |
$883.00
|
|
Citations
The Secreted Protein ANGPTL2 Promotes Metastasis of Osteosarcoma Cells Through Integrin α5β1, p38 MAPK, and Matrix Metalloproteinases
Odagiri, Kadomatsu, Endo et al
Sci Signal (2014) 7 (309), ra7 DOI: 10.1126/scisignal.2004612
Odagiri, Kadomatsu, Endo et al
Sci Signal (2014) 7 (309), ra7 DOI: 10.1126/scisignal.2004612
Cited by 134
AI Summary: OriGene’s pcDNA3.1 vector encoding C-terminally Myc-tagged human TLL1 was used for transient transfection experiments to study the effects of TLL1-mediated cleavage of ANGPTL2 in osteosarcoma cells.
Snippet: 293/ANGPTL2, 293/SD/AA, or 293/Cont cells were transiently transfected with pcDNA3.1 harboring C-terminally Myc-tagged human TLL1 (OriGene) or with vector only
Showing 1-1 of 1 papers.
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