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JMJD7-PLA2G4B (NM_005090) Human Tagged ORF Clone

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SKU
RC223316
JMJD7 (Myc-DDK-tagged)-Human JMJD7-PLA2G4B readthrough (JMJD7-PLA2G4B), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $881.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol JMJD7-PLA2G4B
Synonyms cPLA2-beta; HsT16992
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223316 representing NM_005090
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGCGGCTTTGGAAGCCGTGCGGAGCGAGTTACGAGAATTCCCGGCCGCTGCAAGGGAGCTCT
GCGTGCCTCTTGCTGTGCCCTACCTGGACAAACCCCCAACTCCGCTCCACTTCTACCGGGACTGGGTCTG
CCCCAACAGGCCGTGCATTATCCGCAACGCTCTGCAGCACTGGCCGGCCCTCCAGAAGTGGTCCCTCCCC
TATTTCAGAGCCACAGTGGGCTCCACAGAGGTGAGTGTGGCCGTGACCCCAGATGGTTACGCGGATGCCG
TGAGAGGGGATCGCTTCATGATGCCAGCTGAGCGCCGCCTGCCCCTGAGCTTCGTGCTGGATGTGCTGGA
GGGCCGGGCCCAGCACCCTGGAGTCCTCTATGTGCAGAAGCAGTGCTCCAACCTGCCCAGCGAGCTGCCC
CAGCTGCTGCCTGATCTGGAATCCCATGTGCCCTGGGCCTCCGAAGCCCTGGGAAAGATGCCCGATGCTG
TGAACTTCTGGCTGGGGGAGGCGGCTGCAGTGACTTCTTTGCACAAGGACCACTATGAGAACCTCTACTG
CGTGGTCTCAGGAGAGAAGCATTTCCTGTTCCATCCGCCCAGCGACCGGCCCTTCATCCCCTATGAGCTG
TACACGCCGGCAACCTACCAGCTAACTGAAGAGGGCACCTTTAAGGTGGTGGATGAAGAGGCCATGGAGA
AGGCAGAGGTGTCCAGGACCTGCCTGCTCACGGTTCGTGTCCTGCAGGCCCATCGCCTACCCTCTAAGGA
CCTAGTGACCCCCTCTGACTGCTACGTGACTCTCTGGCTGCCCACGGCCTGCAGCCACAGGCTCCAGACA
CGCACGGTCAAGAACAGCAGTAGCCCTGTCTGGAACCAGAGCTTTCACTTCAGGATCCACAGGCAGCTCA
AGAATGTCATGGAACTGAAAGTCTTTGACCAGGACCTGGTGACCGGAGATGACCCTGTGTTGTCAGTACT
GTTTGATGCGGGGACTCTGCGGGCTGGGGAGTTCCGGCGCGAGAGCTTCTCACTGAGCCCTCAGGGTGAG
GGGCGCCTGGAAGTTGAATTTCGCCTGCAGAGTCTGGCTGACCGTGGCGAGTGGCTCGTCAGCAATGGCG
TTCTGGTGGCCCGGGAGCTCTCCTGCTTGCACGTTCAACTGGAGGAGACAGGAGACCAGAAGTCCTCAGA
GCACAGAGTTCAGCTTGTGGTTCCTGGGTCCTGTGAGGGTCCGCAGGAGGCCTCTGTGGGCACTGGCACC
TTCTGCTTCCACTGCCCAGCCTGCTGGGAGCAGGAGCTGAGTATTCGCCTGCAGGATGCCCCCGAGGAGC
AACTAAAGGCGCCACTGAGTGCCCTGCCCTCTGGTCAAGTGGTGAGGCTTGTCTTCCCCACGTCCCAGGA
GCCCCTGATGAGAGTGGAGCTGAAAAAAGAAGCAGGACTGAGGGAGCTGGCCGTGCGACTGGGCTTCGGG
CCCTGTGCAGAGGAGCAGGCCTTCCTGAGCAGGAGGAAGCAGGTGGTGGCCGCGGCCTTGAGGCAGGCCC
TGCAGCTGGATGGAGACCTGCAGGAGGATGAGATCCCAGTGGTAGCTATTATGGCCACTGGTGGTGGGAT
CCGGGCAATGACTTCCCTGTATGGGCAGCTGGCTGGCCTGAAGGAGCTGGGCCTCTTGGATTGCGTCTCC
TACATCACCGGGGCCTCGGGCTCCACCTGGGCCTTGGCCAACCTTTATGAGGACCCAGAGTGGTCTCAGA
AGGACCTGGCAGGGCCCACTGAGTTGCTGAAGACCCAGGTGACCAAGAACAAGCTGGGTGTGCTGGCCCC
CAGCCAGCTGCAGCGGTACCGGCAGGAGCTGGCCGAGCGTGCCCGCTTGGGCTACCCAAGCTGCTTCACC
AACCTGTGGGCCCTCATCAACGAGGCGCTGCTGCATGATGAGCCCCATGATCACAAGCTCTCAGATCAAC
GGGAGGCCCTGAGTCATGGCCAGAACCCTCTGCCCATCTACTGTGCCCTCAACACCAAAGGGCAGAGCCT
GACCACTTTTGAATTTGGGGAGTGGTGCGAGTTCTCTCCCTACGAGGTCGGCTTCCCCAAGTACGGGGCC
TTCATCCCCTCTGAGCTCTTTGGCTCCGAGTTCTTTATGGGGCAGCTGATGAAGAGGCTTCCTGAGTCCC
GCATCTGCTTCTTAGAAGGTATCTGGAGCAACCTGTATGCAGCCAACCTCCAGGACAGCTTATACTGGGC
CTCAGAGCCCAGCCAGTTCTGGGACCGCTGGGTCAGGAACCAGGCCAACCTGGACAAGGAGCAGGTCCCC
CTTCTGAAGATAGAAGAACCACCCTCAACAGCCGGCAGGATAGCTGAGTTTTTCACCGATCTTCTGACGT
GGCGTCCACTGGCCCAGGCCACACATAATTTCCTGCGTGGCCTCCATTTCCACAAAGACTACTTTCAGCA
TCCTCACTTCTCCACATGGAAAGCTACCACTCTGGATGGGCTCCCCAACCAGCTGACACCCTCGGAGCCC
CACCTGTGCCTGCTGGATGTTGGCTACCTCATCAATACCAGCTGCCTGCCCCTCCTGCAGCCCACTCGGG
ACGTGGACCTCATCCTGTCATTGGACTACAACCTCCACGGAGCCTTCCAGCAGTTGCAGCTCCTGGGCCG
GTTCTGCCAGGAGCAGGGGATCCCGTTCCCACCCATCTCGCCCAGCCCCGAAGAGCAGCTCCAGCCTCGG
GAGTGCCACACCTTCTCCGACCCCACCTGCCCCGGAGCCCCTGCGGTGCTGCACTTTCCTCTGGTCAGCG
ACTCCTTCCGGGAGTACTCGGCCCCTGGGGTCCGGCGGACACCCGAGGAGGCGGCAGCTGGGGAGGTGAA
CCTGTCTTCATCGGACTCTCCCTACCACTACACGAAGGTGACCTACAGCCAGGAGGACGTGGACAAGCTG
CTGCACCTGACACATTACAATGTCTGCAACAACCAGGAGCAGCTGCTGGAGGCTCTGCGCCAGGCAGTGC
AGCGGAGGCGGCAGCGCAGGCCCCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223316 representing NM_005090
Red=Cloning site Green=Tags(s)

MAEAALEAVRSELREFPAAARELCVPLAVPYLDKPPTPLHFYRDWVCPNRPCIIRNALQHWPALQKWSLP
YFRATVGSTEVSVAVTPDGYADAVRGDRFMMPAERRLPLSFVLDVLEGRAQHPGVLYVQKQCSNLPSELP
QLLPDLESHVPWASEALGKMPDAVNFWLGEAAAVTSLHKDHYENLYCVVSGEKHFLFHPPSDRPFIPYEL
YTPATYQLTEEGTFKVVDEEAMEKAEVSRTCLLTVRVLQAHRLPSKDLVTPSDCYVTLWLPTACSHRLQT
RTVKNSSSPVWNQSFHFRIHRQLKNVMELKVFDQDLVTGDDPVLSVLFDAGTLRAGEFRRESFSLSPQGE
GRLEVEFRLQSLADRGEWLVSNGVLVARELSCLHVQLEETGDQKSSEHRVQLVVPGSCEGPQEASVGTGT
FCFHCPACWEQELSIRLQDAPEEQLKAPLSALPSGQVVRLVFPTSQEPLMRVELKKEAGLRELAVRLGFG
PCAEEQAFLSRRKQVVAAALRQALQLDGDLQEDEIPVVAIMATGGGIRAMTSLYGQLAGLKELGLLDCVS
YITGASGSTWALANLYEDPEWSQKDLAGPTELLKTQVTKNKLGVLAPSQLQRYRQELAERARLGYPSCFT
NLWALINEALLHDEPHDHKLSDQREALSHGQNPLPIYCALNTKGQSLTTFEFGEWCEFSPYEVGFPKYGA
FIPSELFGSEFFMGQLMKRLPESRICFLEGIWSNLYAANLQDSLYWASEPSQFWDRWVRNQANLDKEQVP
LLKIEEPPSTAGRIAEFFTDLLTWRPLAQATHNFLRGLHFHKDYFQHPHFSTWKATTLDGLPNQLTPSEP
HLCLLDVGYLINTSCLPLLQPTRDVDLILSLDYNLHGAFQQLQLLGRFCQEQGIPFPPISPSPEEQLQPR
ECHTFSDPTCPGAPAVLHFPLVSDSFREYSAPGVRRTPEEAAAGEVNLSSSDSPYHYTKVTYSQEDVDKL
LHLTHYNVCNNQEQLLEALRQAVQRRRQRRPH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005090
ORF Size 3036 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005090.3
RefSeq Size 3352 bp
RefSeq ORF 3039 bp
Locus ID 8681
UniProt ID P0C869, P0C869-6
Cytogenetics 15q15.1
Protein Pathways alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
MW 113.9 kDa
Summary This locus represents naturally-occurring readthrough transcription between the neighboring jumonji domain containing 7 (JMJD7) and phospholipase A2, group IVB (cytosolic) (PLA2G4B) genes. Readthrough transcripts encode fusion proteins that share amino acid sequence with each individual gene product, including a partial JmjC domain and downstream C2 and phospholipase A2 domains. Alternatively spliced transcript variants have been observed. provided by RefSeq, Oct 2013
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Documents
Resources
"NM_005090" in other vectors (4)
SKU Description Size Price
RC223316L3 Lenti ORF clone of Human JMJD7-PLA2G4B readthrough (JMJD7-PLA2G4B), transcript variant 1, Myc-DDK-tagged 10 ug
$1,181.00
RC223316L4 Lenti ORF clone of Human JMJD7-PLA2G4B readthrough (JMJD7-PLA2G4B), transcript variant 1, mGFP tagged 10 ug
$1,181.00
RG223316 JMJD7 (tGFP-tagged) - Human JMJD7-PLA2G4B readthrough (JMJD7-PLA2G4B), transcript variant 1 10 ug
$1,081.00
SC303580 JMJD7 (untagged)-Human JMJD7-PLA2G4B readthrough (JMJD7-PLA2G4B), transcript variant 1 10 ug
$882.00

Plasmid Map for RC223316

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223316
Circular map for RC223316

file-alt Citations

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