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MAP3K15 (NM_001001671) Human Tagged ORF Clone

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SKU
RC223307
MAP3K15 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 15 (MAP3K15)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $722.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAP3K15
Synonyms ASK3; bA723P2.3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223307 representing NM_001001671
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATGTCTCACCCACAGAAATGAAACAGATGCACGAATGGAATTTTACAGCCTCTTCCATAAAGGGA
ATAAGGCTGGAGTACAGTGGCACGATCTCGGCTCACTGCAACCTCTGCCTCCCAGGTTCAAGCGATTCTC
CTGCCTCAGCCTCCAGAGTAGCTGGGACTACAGCCTATCAAAGTTTGATGAAAGGTGTTGTTTTCTTTAT
GTCCATGATAATTCTGATGACTTTCAAATCTACTTTTCCACCGAAGAGCAGTGCAGTAGATTTTTCTCTT
TGGTCAAAGAGATGATAACCAATACAGCAGGCAGTACGGTGGAGCTGGAGGGAGAGACCGATGGAGACAC
CTTGGAGTATGAGTATGACCATGATGCAAATGGTGAGAGAGTTGTCTTGGGGAAAGGCACGTATGGGATT
GTGTATGCTGGCCGAGATCTGAGCAATCAAGTGCGAATAGCCATCAAAGAAATCCCGGAGAGAGATAGCA
GGTATTCTCAGCCTCTGCACGAGGAGATAGCCCTGCACAAGTACCTTAAGCACCGCAATATCGTTCAGTA
CCTGGGCTCTGTTTCAGAGAACGGCTACATTAAGATATTTATGGAGCAGGTGCCTGGAGGAAGCCTTTCT
GCTCTTCTGCGATCCAAATGGGGGCCGATGAAGGAACCGACAATCAAGTTTTACACCAAACAGATCCTGG
AGGGCCTTAAGTATCTTCATGAAAACCAGATCGTGCACAGAGACATAAAGGGCGATAATGTTCTGGTGAA
CACCTACAGCGGAGTGGTGAAAATCTCCGATTTTGGAACCTCGAAACGTCTTGCGGGTGTGAACCCCTGC
ACAGAGACTTTTACTGGCACCCTGCAGTACATGGCACCTGAGATAATTGACCAAGGGCCTCGCGGATATG
GTGCCCCAGCCGATATCTGGTCCCTGGGCTGCACCATCATTGAGATGGCCACCAGCAAGCCTCCGTTCCA
TGAGCTTGGTGAGCCGCAGGCAGCCATGTTCAAAGTGGGCATGTTTAAGATCCACCCTGAGATTCCAGAA
GCCCTTTCAGCTGAAGCCCGAGCCTTCATTTTATCCTGTTTCGAGCCTGACCCCCACAAACGTGCCACCA
CTGCTGAGCTACTGAGAGAGGGTTTCTTAAGGCAGGTGAACAAGGGCAAGAAGAACCGAATTGCCTTCAA
GCCCTCAGAAGGTCCCCGCGGTGTCGTCCTGGCCCTGCCCACACAGGGAGAGCCCATGGCCACCAGCAGC
AGCGAGCACGGCTCTGTCTCCCCAGACTCCGACGCCCAGCCTGACGCACTCTTTGAGAGGACCCGGGCGC
CCAGGCACCACCTTGGCCACCTCCTCAGTGTTCCAGACGAGAGCTCAGCCTTGGAAGACCGGGGCTTGGC
CTCGTCCCCGGAGGACAGGGACCAGGGCCTCTTCCTGCTACGCAAGGACAGTGAGCGCCGTGCCATCCTG
TACAAAATCCTCTGGGAGGAGCAGAACCAGGTGGCTTCCAACCTGCAGGAGTGTGTGGCCCAGAGTTCCG
AAGAGTTGCATCTCTCAGTTGGACACATCAAGCAAATCATTGGGATCCTGAGGGACTTCATCCGCTCCCC
AGAGCACCGGGTGATGGCGACCACAATATCAAAGCTCAAGGTGGACCTGGACTTTGACAGCTCGTCCATC
AGTCAGATTCACCTGGTGCTGTTCGGATTTCAGGATGCCGTAAATAAAATTTTGAGGAACCACTTAATTA
GGCCCCACTGGATGTTCGCGATGGACAACATCATCCGCCGAGCGGTGCAGGCCGCGGTCACCATTCTCAT
CCCAGAGCTCCGAGCCCACTTTGAGCCTACCTGTGAGACTGAAGGGGTAGATAAGGACATGGATGAAGCG
GAAGAGGGCTATCCCCCAGCCACCGGACCTGGCCAGGAGGCCCAGCCCCACCAGCAGCACCTGAGCCTCC
AGCTGGGTGAGCTCAGACAGGAGACCAACAGACTTTTGGAACACCTAGTTGAAAAAGAGAGAGAGTACCA
GAATCTTCTGCGGCAAACTCTAGAACAGAAAACTCAAGAATTGTATCACCTTCAGTTAAAATTAAAATCG
AATTGTATTACAGAGAACCCAGCAGGCCCCTACGGGCAGAGAACAGATAAAGAGCTTATAGACTGGTTGC
GGCTGCAAGGAGCTGATGCAAAGACAATTGAAAAGATTGTTGAAGAGGGTTATACACTTTCGGATATTCT
TAATGAGATCACTAAGGAAGATCTAAGATACCTTCGACTACGGGGTGGTCTCCTCTGCAGACTCTGGAGT
GCGGTCTCCCAGTACAGAAGGGCTCAGGAGGCCTCAGAAACCAAAGACAAGGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223307 representing NM_001001671
Red=Cloning site Green=Tags(s)

MACLTHRNETDARMEFYSLFHKGNKAGVQWHDLGSLQPLPPRFKRFSCLSLQSSWDYSLSKFDERCCFLY
VHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGI
VYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLS
ALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPC
TETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPE
ALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSS
SEHGSVSPDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAIL
YKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSI
SQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEA
EEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKS
NCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWS
AVSQYRRAQEASETKDKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001001671
ORF Size 2364 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001001671.2
RefSeq Size 4633 bp
RefSeq ORF 3942 bp
Locus ID 389840
UniProt ID Q6ZN16
Cytogenetics Xp22.12
Protein Families Druggable Genome, Protein Kinase
MW 89.4 kDa
Summary The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAPK) family. These family members function in a protein kinase signal transduction cascade, where an activated MAPK kinase kinase (MAP3K) phosphorylates and activates a specific MAPK kinase (MAP2K), which then activates a specific MAPK. This MAP3K protein plays an essential role in apoptotic cell death triggered by cellular stresses. provided by RefSeq, Jul 2010
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"NM_001001671" in other vectors (6)
SKU Description Size Price
RC223307L1 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 15 (MAP3K15), Myc-DDK-tagged 10 ug
$1,022.00
RC223307L2 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 15 (MAP3K15), mGFP tagged 10 ug
$1,022.00
RC223307L3 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 15 (MAP3K15), Myc-DDK-tagged 10 ug
$1,022.00
RC223307L4 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 15 (MAP3K15), mGFP tagged 10 ug
$1,022.00
RG223307 MAP3K15 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 15 (MAP3K15) 10 ug
$789.00 $922.00
SC300256 MAP3K15 (untagged)-Human mitogen-activated protein kinase kinase kinase 15 (MAP3K15) 10 ug
$722.00

Plasmid Map for RC223307

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223307
Circular map for RC223307

file-alt Citations

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