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POLD3 (NM_006591) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC223072L3
Lenti ORF clone of Human polymerase (DNA-directed), delta 3, accessory subunit (POLD3), Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $986.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol POLD3
Synonyms P66; P68; PPP1R128
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC223072).
Restriction Sites SgfI-RsrII Cloning Scheme for this gene |
ACCN NM_006591
ORF Size 1398 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006591.1
RefSeq Size 3430 bp
RefSeq ORF 1401 bp
Locus ID 10714
UniProt ID Q15054
Cytogenetics 11q13.4
Protein Pathways Base excision repair, DNA replication, Homologous recombination, Metabolic pathways, Mismatch repair, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
MW 51.2 kDa
Summary This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. provided by RefSeq, Mar 2012
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"NM_006591" in other vectors (4)
SKU Description Size Price
RC223072 POLD3 (Myc-DDK-tagged)-Human polymerase (DNA-directed), delta 3, accessory subunit (POLD3) 10 ug
$686.00
RC223072L4 Lenti ORF clone of Human polymerase (DNA-directed), delta 3, accessory subunit (POLD3), mGFP tagged 10 ug
$986.00
RG223072 POLD3 (tGFP-tagged) - Human polymerase (DNA-directed), delta 3, accessory subunit (POLD3) 10 ug
$886.00
SC303795 POLD3 (untagged)-Human polymerase (DNA-directed), delta 3, accessory subunit (POLD3) 10 ug
$732.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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