OR5B17 (NM_001005489) Human Tagged ORF Clone

  • TrueORF®
SKU
RC223023
OR5B17 (Myc-DDK-tagged)-Human olfactory receptor, family 5, subfamily B, member 17 (OR5B17)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OR5B17
Synonyms OR5B20P; OR11-237
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223023 representing NM_001005489
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAATAATACAGAGGTGAGTGAATTCATCCTGCTTGGTCTAACCAATGCCCCAGAACTACAGGTTC
CCCTCTTTATCATGTTTACCCTCATCTACCTCATCACTCTGACTGGGAACCTGGGGATGATCATATTAAT
CCTGCTGGACTCTCATCTCCACACTCCCATGTACTTTTTTCTCAGTAACCTGTCTCTTGCAGGCATTGGT
TACTCCTCAGCTGTCACTCCAAAGGTTTTAACTGGGTTGCTTATAGAAGACAAAGCCATCTCCTACAGTG
CCTGTGCTGCTCAGATGTTCTTTTGTGCAGTCTTTGCCACTGTGGAAAATTACCTCTTGTCCTCAATGGC
CTATGACCGCTACGCAGCAGTGTGTAACCCCCTACATTATACCACCACCATGACAACACGTGTGTGTGCT
TGTCTGGCTATAGGCTGTTATGTCATTGGTTTTCTGAATGCTTCTATCCAAATTGGAGATACATTTCGCC
TCTCTTTCTGCATGTCCAATGTGATTCATCACTTTTTCTGTGACAAACCAGCAGTCATTACTCTGACCTG
CTCTGAGAAACACATTAGTGAGTTGATTCTTGTTCTTATATCAAGTTTTAATGTCTTTTTTGCACTTCTT
GTTACCTTGATTTCCTATCTGTTCATATTGATCACCATTCTTAAGAGGCACACAGGTAAGGGATACCAGA
AGCCTTTATCTACCTGTGGTTCTCACCTCATTGCCATTTTCTTATTTTATATAACTGTCATCATCATGTA
CATACGACCAAGTTCCAGTCATTCCATGGACACAGACAAAATTGCATCTGTGTTCTACACTATGATCATC
CCCATGCTCAGTCCTATAGTCTATACCCTGAGGAACAAAGACGTGAAGAATGCATTCATGAAGGTTGTTG
AGAAGGCAAAATATTCTCTAGATTCAGTCTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC223023 representing NM_001005489
Red=Cloning site Green=Tags(s)

MENNTEVSEFILLGLTNAPELQVPLFIMFTLIYLITLTGNLGMIILILLDSHLHTPMYFFLSNLSLAGIG
YSSAVTPKVLTGLLIEDKAISYSACAAQMFFCAVFATVENYLLSSMAYDRYAAVCNPLHYTTTMTTRVCA
CLAIGCYVIGFLNASIQIGDTFRLSFCMSNVIHHFFCDKPAVITLTCSEKHISELILVLISSFNVFFALL
VTLISYLFILITILKRHTGKGYQKPLSTCGSHLIAIFLFYITVIIMYIRPSSSHSMDTDKIASVFYTMII
PMLSPIVYTLRNKDVKNAFMKVVEKAKYSLDSVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005489
ORF Size 942 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005489.2
RefSeq Size 945 bp
RefSeq ORF 945 bp
Locus ID 219965
UniProt ID Q8NGF7
Cytogenetics 11q12.1
Protein Families Transmembrane
Protein Pathways Olfactory transduction
MW 34.9 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001005489" in other vectors (4)
SKU Description Size Price
RC223023L3 Lenti ORF clone of Human olfactory receptor, family 5, subfamily B, member 17 (OR5B17), Myc-DDK-tagged 10 ug
$600.00
RC223023L4 Lenti ORF clone of Human olfactory receptor, family 5, subfamily B, member 17 (OR5B17), mGFP tagged 10 ug
$600.00
RG223023 OR5B17 (tGFP-tagged) - Human olfactory receptor, family 5, subfamily B, member 17 (OR5B17) 10 ug
$500.00
SC300972 OR5B17 (untagged)-Human olfactory receptor, family 5, subfamily B, member 17 (OR5B17) 10 ug
$330.00

Plasmid Map for RC223023

Download sequence for

Cloning scheme for RC223023

Circular map for RC223023

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