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FBXO17 (NM_148169) Human Tagged ORF Clone

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SKU
RC222996
FBXO17 (Myc-DDK-tagged)-Human F-box protein 17 (FBXO17), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXO17
Synonyms FBG4; Fbx17; FBX26; FBXO26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222996 representing NM_148169
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCAAGGACTCTGGCTACTGGAGATGGGCGCCCGGCTATCGCGGCGACGGCTGCCGGCGGACCCAT
CCCTGGCCCTGGACGCGCTGCCCCCGGAGCTGCTGGTGCAGGTGCTGAGCCACGTGCCGCCACGCTCCTT
GGTCACGCGATGCCGCCCAGTGTGCCGCGCCTGGCGCGACATAGTGGACGGGCCCACTGTGTGGCTGCTG
CAGCTGGCCCGCGACCGCAGCGCCGAGGGCCGCGCACTCTACGCAGTGGCTCAACGCTGCCTGCCCAGCA
ACGAAGACAAGGAGGAGTTCCCGCTGTGCGCCCTGGCGCGCTACTGTCTGCGCGCGCCCTTCGGCCGCAA
TCTCATCTTCAACTCCTGCGGAGAGCAGGGCTTCAGAGGCTGGGAGGTGGAGCATGGCGGGAACGGCTGG
GCCATAGAAAAGAACCTAACACCGGTGCCTGGGGCTCCTTCGCAGACCTGCTTCGTGACCTCTTTCGAAT
GGTGCTCCAAGAGGCAGCTTGTGGACCTGGTGATGGAAGGGGTGTGGCAGGAGCTGCTGGACAGCGCCCA
GATTGAGATCTGTGTGGCTGACTGGTGGGGCGCTCGAGAGAACTGCGGCTGCGTCTACCAGCTCCGGGTC
CGCCTTCTGGATGTGTATGAAAAGGAAGTGGTCAAGTTCTCAGCCTCACCTGACCCGGTCCTTCAGTGGA
CTGAGAGGGGCTGCCGACAGGTCTCCCACGTCTTCACCAACTTTGGCAAGGGCATCCGCTACGTATCTTT
TGAGCAGTACGGGAGAGACGTGAGTTCCTGGGTGGGGCACTATGGCGCCCTTGTGACCCACTCCAGTGTG
AGGGTCAGGATCCGTCTGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222996 representing NM_148169
Red=Cloning site Green=Tags(s)

MKQGLWLLEMGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLL
QLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGW
AIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRV
RLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSV
RVRIRLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_148169
ORF Size 861 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_148169.2, NP_680474.1
RefSeq Size 2175 bp
RefSeq ORF 864 bp
Locus ID 115290
UniProt ID Q96EF6
Cytogenetics 19q13.2
Domains F-box, FBA
Protein Families Druggable Genome
MW 32.4 kDa
Summary This gene encodes a member of the F-box protein family which is characterized by the F-box motif. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it contains an F-box domain. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2013
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"NM_148169" in other vectors (6)
SKU Description Size Price
RC222996L1 Lenti ORF clone of Human F-box protein 17 (FBXO17), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC222996L2 Lenti ORF clone of Human F-box protein 17 (FBXO17), transcript variant 1, mGFP tagged 10 ug
$600.00
RC222996L3 Lenti ORF clone of Human F-box protein 17 (FBXO17), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC222996L4 Lenti ORF clone of Human F-box protein 17 (FBXO17), transcript variant 1, mGFP tagged 10 ug
$600.00
RG222996 FBXO17 (tGFP-tagged) - Human F-box protein 17 (FBXO17), transcript variant 1 10 ug
$500.00
SC310570 FBXO17 (untagged)-Human F-box protein 17 (FBXO17), transcript variant 1 10 ug
$330.00

Plasmid Map for RC222996

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222996
Circular map for RC222996

file-alt Citations

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