DNASE1L2 (NM_001374) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC222905
DNASE1L2 (Myc-DDK-tagged)-Human deoxyribonuclease I-like 2 (DNASE1L2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNASE1L2
Synonyms DNAS1L2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222905 representing NM_001374
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGGGCCCCGGGCTCTGCTGGCCGCACTCTGGGCGCTGGAAGCCGCCGGGACCGCCGCGCTTCGCA
TCGGAGCCTTCAACATTCAGAGCTTCGGTGACAGCAAAGTGTCGGACCCCGCTTGCGGCAGCATCATCGC
GAAGATCCTGGCTGGCTATGACCTCGCGCTGGTGCAGGAGGTGCGAGACCCAGACCTCAGCGCCGTGTCC
GCGCTCATGGAGCAGATCAACAGCGTGTCCGAGCACGAGTACAGCTTTGTGAGCAGCCAGCCCCTGGGCC
GGGACCAGTACAAGGAGATGTACCTGTTCGTGTACAGGAAAGACGCGGTGTCGGTCGTGGACACCTACCT
GTACCCAGACCCCGAGGACGTCTTCAGCCGCGAGCCCTTCGTGGTCAAGTTCTCGGCCCCCGGCACCGGT
GAGCGGGCCCCGCCCCTCCCCTCCCGCCGAGCTCTGACGCCCCCACCCCTTCCCGCAGCAGCACAGAACC
TGGTGCTGATCCCGCTGCACGCGGCGCCGCATCAAGCCGTGGCGGAGATCGACGCGCTCTACGACGTGTA
CCTGGACGTGATCGACAAGTGGGGCACCGACGACATGCTGTTCCTGGGCGACTTCAACGCCGACTGCAGC
TATGTGCGGGCGCAGGACTGGGCCGCCATCCGTCTGAGGAGCAGTGAGGTCTTCAAGTGGCTCATCCCTG
ACAGCGCCGACACCACGGTGGGCAACTCAGACTGCGCCTACGACCGCATTGTGGCCTGTGGCGCCCGCCT
GCGCCGGAGCCTGAAGCCCCAGTCGGCCACCGTGCACGACTTCCAGGAGGAATTCGGCCTGGACCAGACT
CAGGCTCTTGCCATCAGCGACCACTTTCCAGTGGAGGTGACCCTCAAGTTCCACCGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC222905 representing NM_001374
Red=Cloning site Green=Tags(s)

MGGPRALLAALWALEAAGTAALRIGAFNIQSFGDSKVSDPACGSIIAKILAGYDLALVQEVRDPDLSAVS
ALMEQINSVSEHEYSFVSSQPLGRDQYKEMYLFVYRKDAVSVVDTYLYPDPEDVFSREPFVVKFSAPGTG
ERAPPLPSRRALTPPPLPAAAQNLVLIPLHAAPHQAVAEIDALYDVYLDVIDKWGTDDMLFLGDFNADCS
YVRAQDWAAIRLRSSEVFKWLIPDSADTTVGNSDCAYDRIVACGARLRRSLKPQSATVHDFQEEFGLDQT
QALAISDHFPVEVTLKFHR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001374
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001374.3
RefSeq Size 1365 bp
RefSeq ORF 900 bp
Locus ID 1775
UniProt ID Q92874
Cytogenetics 16p13.3
Protein Families Secreted Protein
MW 32.7 kDa
Summary Divalent cation-dependent acid DNA endonuclease involved in the breakdown of the nucleus during corneocyte formation of epidermal keratinocytes. May play an immune role by eliminating harmful DNA released into the extracellular environment by damaged epidermal cells.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001374" in other vectors (4)
SKU Description Size Price
RC222905L3 Lenti ORF clone of Human deoxyribonuclease I-like 2 (DNASE1L2), Myc-DDK-tagged 10 ug
$600.00
RC222905L4 Lenti ORF clone of Human deoxyribonuclease I-like 2 (DNASE1L2), mGFP tagged 10 ug
$600.00
RG222905 DNASE1L2 (tGFP-tagged) - Human deoxyribonuclease I-like 2 (DNASE1L2) 10 ug
$500.00
SC122592 DNASE1L2 (untagged)-Human deoxyribonuclease I-like 2 (DNASE1L2) 10 ug
$300.00

Plasmid Map for RC222905

Download sequence for

Cloning scheme for RC222905

Circular map for RC222905

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products