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GALNT8 (NM_017417) Human Tagged ORF Clone

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SKU
RC222849
GALNT8 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8) (GALNT8)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $593.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GALNT8
Synonyms GALNAC-T8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222849 representing NM_017417
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTTTTGGAGGAAACTCCCCAAAGCCCTCTTCATTGGGCTGACTCTGGCCATTGCTGTCAATCTCC
TTCTGGTATTTTCTAGCAAGGGGACTTTACAAAACCTGTTTACGGGTGGTCTCCACAGGGAGCTTCCTTT
ACATCTGAATAAACGCTACGGGGCAGTGATAAAGAGACTCTCCCACTTGGAGGTGGAATTGCAGGATCTG
AAAGAAAGTATGAAATTAGCTCTGAGGCAACAAGAAAATGTGAACAGCACACTGAAGAGGGCGAAAGATG
AAGTACGCCCTCTTCTAAAGGCAATGGAAACCAAGGTGAATGAGACAAAGAAGCACAAAACCCAAATGAA
ACTCTTCCCACACTCACAGCTTTTCAGGCAATGGGGCGAGGATCTTTCTGAGGCCCAGCAGAAGGCGGCC
CAGGACCTCTTCCGGAAGTTTGGTTACAACGCGTACCTCAGCAACCAGCTGCCTCTCAATCGCACCATCC
CCGACACGCGAGACTACAGATGTCTTCGGAAGACATATCCTTCCCAACTCCCATCCCTCAGTGTCATTCT
CATATTCGTGAATGAAGCTCTGTCCATTATACAACGGGCCATCACCAGTATCATCAACCGGACGCCCTCT
CGATTGTTGAAGGAAATCATCTTGGTGGATGATTTCAGCTCAAATGGAGAACTAAAGGTACACTTGGATG
AGAAGATTAAGCTTTACAACCAGAAGTATCCAGGACTACTGAAAATAATACGGCATCCTGAAAGGAAAGG
TCTTGCTCAAGCCCGCAACACTGGCTGGGAAGCTGCCACAGCAGACGTGGTCGCCATCTTGGATGCTCAC
ATTGAAGTCAATGTTGGGTGGGCAGAGCCAATCTTGGCTCGGATTCAGGAGGACCGCACTGTGATTGTGT
CTCCTGTGTTTGACAACATTCGTTTTGACACCTTCAAACTGGATAAGTATGAACTGGCAGTTGATGGGTT
TAACTGGGAACTCTGGTGCCGCTACGATGCACTGCCACAAGCCTGGATTGATCTGCATGATGTCACTGCC
CCAGTGAAGAGTCCTTCAATCATGGGCATCCTGGCTGCTAACAGGCACTTCCTGGGAGAGATCGGGTCTC
TGGATGGTGGAATGCTCATCTATGGAGGAGAGAACGTGGAGCTTAGCCTGAGGGTGTGGCAGTGTGGAGG
GAAGGTCGAGATTTTGCCCTGTTCCCGGATTGCCCACCTAGAGAGACACCACAAGCCCTACGCCTTGGAT
CTCACCGCTGCCTTGAAGCGCAATGCTCTGCGAGTGGCCGAAATCTGGATGGATGAGCACAAACACATGG
TCTACTTGGCCTGGAACATACCTCTCCAGAACTCTGGAATAGATTTTGGAGACGTTTCTTCCAGAATGGC
ACTCCGGGAAAAACTGAAATGTAAAACTTTTGACTGGTACCTGAAAAATGTTTATCCACTCTTGAAGCCA
CTCCACACCATCGTGGGCTATGGAAGAATGAAAAACCTATTGGATGAAAATGTCTGCTTGGATCAGGGAC
CCGTTCCAGGCAACACCCCCATCATGTATTACTGCCATGAATTCAGCTCACAGAATGTCTACTATCACCT
AACTGGGGAGCTCTATGTGGGACAACTGATTGCAGAGGCCAGTGCTAGTGATCGCTGCCTGACAGACCCT
GGCAAGGCGGAGAAGCCCACCTTAGAACCATGCTCCAAGGCAGCTAAGAATAGACTGCATATATATTGGG
ATTTTAAACCGGGAGGAGCTGTCATAAACAGAGATACCAAGCGGTGTCTGGAGATGAAGAAGGATCTTTT
GGGTAGCCACGTGCTTGTGCTCCAGACCTGTAGCACGCAAGTGTGGGAAATCCAGCACACTGTCAGAGAC
TGGGGTCAGACCAACAGCCAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222849 representing NM_017417
Red=Cloning site Green=Tags(s)

MMFWRKLPKALFIGLTLAIAVNLLLVFSSKGTLQNLFTGGLHRELPLHLNKRYGAVIKRLSHLEVELQDL
KESMKLALRQQENVNSTLKRAKDEVRPLLKAMETKVNETKKHKTQMKLFPHSQLFRQWGEDLSEAQQKAA
QDLFRKFGYNAYLSNQLPLNRTIPDTRDYRCLRKTYPSQLPSLSVILIFVNEALSIIQRAITSIINRTPS
RLLKEIILVDDFSSNGELKVHLDEKIKLYNQKYPGLLKIIRHPERKGLAQARNTGWEAATADVVAILDAH
IEVNVGWAEPILARIQEDRTVIVSPVFDNIRFDTFKLDKYELAVDGFNWELWCRYDALPQAWIDLHDVTA
PVKSPSIMGILAANRHFLGEIGSLDGGMLIYGGENVELSLRVWQCGGKVEILPCSRIAHLERHHKPYALD
LTAALKRNALRVAEIWMDEHKHMVYLAWNIPLQNSGIDFGDVSSRMALREKLKCKTFDWYLKNVYPLLKP
LHTIVGYGRMKNLLDENVCLDQGPVPGNTPIMYYCHEFSSQNVYYHLTGELYVGQLIAEASASDRCLTDP
GKAEKPTLEPCSKAAKNRLHIYWDFKPGGAVINRDTKRCLEMKKDLLGSHVLVLQTCSTQVWEIQHTVRD
WGQTNSQ

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_017417
ORF Size 1911 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017417.2
RefSeq Size 2135 bp
RefSeq ORF 1914 bp
Locus ID 26290
UniProt ID Q9NY28
Cytogenetics 12p13.32
Protein Families Transmembrane
Protein Pathways Metabolic pathways, O-Glycan biosynthesis
MW 72.7 kDa
Summary This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. provided by RefSeq, Jul 2008
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"NM_017417" in other vectors (4)
SKU Description Size Price
RC222849L3 Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8) (GALNT8), Myc-DDK-tagged 10 ug
$893.00
RC222849L4 Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8) (GALNT8), mGFP tagged 10 ug
$893.00
RG222849 GALNT8 (tGFP-tagged) - Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8) (GALNT8) 10 ug
$793.00
SC304459 GALNT8 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8) (GALNT8) 10 ug
$594.00

Plasmid Map for RC222849

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222849
Circular map for RC222849

file-alt Citations

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