Rapsyn (RAPSN) (NM_005055) Human Tagged ORF Clone

SKU
RC222710
RAPSN (Myc-DDK-tagged)-Human receptor-associated protein of the synapse (RAPSN), transcript variant 1
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Rapsyn
Synonyms CMS4C; CMS11; FADS; FADS2; RAPSYN; RNF205
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222710 representing NM_005055
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAGGACCAGACCAAGAAGCAGATCGAGAAGGGGCTCCAGCTGTACCAGTCCAACCAGACAGAGA
AGGCATTGCAGGTGTGGACAAAGGTGCTGGAGAAGAGCTCGGACCTCATGGGGCGCTTCCGCGTGCTGGG
CTGCCTGGTCACAGCCCACTCGGAGATGGGCCGCTACAAGGAGATGCTGAAGTTCGCTGTGGTCCAGATC
GACACGGCCCGGGAGCTGGAGGATGCCGACTTCCTCCTGGAGAGCTACCTGAACCTGGCACGCAGCAACG
AGAAGCTGTGCGAGTTTCACAAGACCATCTCCTACTGCAAGACCTGCCTTGGGCTGCCTGGTACCAGGGC
AGGTGCCCAGCTCGGAGGCCAGGTCAGCCTGAGCATGGGCAATGCCTTCCTGGGCCTCAGCGTCTTCCAG
AAGGCCCTGGAGAGCTTCGAGAAGGCCCTGCGCTATGCCCACAACAATGATGACGCCATGCTCGAGTGCC
GCGTGTGCTGCAGCCTGGGCAGCTTCTATGCCCAGGTCAAGGACTACGAGAAAGCCCTGTTCTTCCCCTG
CAAGGCGGCAGAGCTTGTCAACAACTATGGCAAAGGCTGGAGCCTGAAGTACCGGGCCATGAGCCAGTAC
CACATGGCCGTGGCCTATCGCCTGCTGGGCCGCCTGGGCAGTGCCATGGAGTGTTGTGAGGAGTCTATGA
AGATCGCGCTGCAGCACGGGGACCGGCCACTGCAGGCGCTCTGCCTGCTCTGCTTCGCTGACATCCACCG
GAGCCGTGGGGACCTGGAGACAGCCTTCCCCAGGTACGACTCCGCCATGAGCATCATGACCGAGATCGGA
AACCGCCTGGGGCAGGTGCAGGCGCTGCTGGGTGTGGCCAAGTGCTGGGTGGCCAGGAAGGCGCTGGACA
AGGCTCTGGATGCCATCGAGAGAGCCCAGGATCTGGCCGAGGAGGTGGGGAACAAGCTGAGCCAGCTCAA
GCTGCACTGTCTGAGCGAGAGCATTTACCGCAGCAAAGGGCTGCAGCGGGAACTGCGGGCGCACGTTGTG
AGGTTCCACGAGTGCGTGGAGGAGACGGAGCTCTACTGCGGCCTGTGCGGCGAGTCCATAGGCGAGAAGA
ACAGCCGGCTGCAGGCCCTACCTTGCTCCCACATCTTCCACCTCAGGTGCCTGCAGAACAACGGGACCCG
GAGCTGTCCCAACTGCCGCCGCTCATCCATGAAGCCTGGCTTTGTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC222710 representing NM_005055
Red=Cloning site Green=Tags(s)

MGQDQTKKQIEKGLQLYQSNQTEKALQVWTKVLEKSSDLMGRFRVLGCLVTAHSEMGRYKEMLKFAVVQI
DTARELEDADFLLESYLNLARSNEKLCEFHKTISYCKTCLGLPGTRAGAQLGGQVSLSMGNAFLGLSVFQ
KALESFEKALRYAHNNDDAMLECRVCCSLGSFYAQVKDYEKALFFPCKAAELVNNYGKGWSLKYRAMSQY
HMAVAYRLLGRLGSAMECCEESMKIALQHGDRPLQALCLLCFADIHRSRGDLETAFPRYDSAMSIMTEIG
NRLGQVQALLGVAKCWVARKALDKALDAIERAQDLAEEVGNKLSQLKLHCLSESIYRSKGLQRELRAHVV
RFHECVEETELYCGLCGESIGEKNSRLQALPCSHIFHLRCLQNNGTRSCPNCRRSSMKPGFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_005055
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005055.5
RefSeq Size 1664 bp
RefSeq ORF 1239 bp
Locus ID 5913
UniProt ID Q13702
Cytogenetics 11p11.2
Protein Families Druggable Genome
MW 46.1 kDa
Summary This gene encodes a member of a family of proteins that are receptor associated proteins of the synapse. The encoded protein contains a conserved cAMP-dependent protein kinase phosphorylation site, and plays a critical role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites by linking the receptors to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Mutations in this gene may play a role in postsynaptic congenital myasthenic syndromes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011]
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SKU Description Size Price
RC222710L1 Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC222710L2 Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, mGFP tagged 10 ug
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RC222710L3 Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC222710L4 Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, mGFP tagged 10 ug
$757.00
RG222710 RAPSN (tGFP-tagged) - Human receptor-associated protein of the synapse (RAPSN), transcript variant 1 10 ug
$489.00 MSRP $657.00 MSRP $657.00
SC303569 RAPSN (untagged)-Human receptor-associated protein of the synapse (RAPSN), transcript variant 1 10 ug
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Citations

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