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PGGT1B (NM_005023) Human Tagged ORF Clone

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SKU
RC222599
PGGT1B (Myc-DDK-tagged)-Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PGGT1B
Synonyms BGGI; GGTI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222599 representing NM_005023
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCCACTGAGGATGAGAGGCTAGCAGGGAGCGGTGAGGGAGAGCGGCTGGATTTCTTACGGGATC
GGCACGTGCGATTTTTCCAGCGCTGCCTCCAGGTTTTGCCGGAGCGCTATTCTTCACTCGAGACAAGCAG
GTTGACAATTGCATTTTTTGCACTCTCCGGGCTGGATATGTTGGATTCCTTAGATGTGGTGAACAAAGAT
GATATAATAGAGTGGATTTATTCCCTGCAGGTCCTTCCCACAGAAGACAGATCAAATCTAAATCGCTGTG
GTTTCCGAGGCTCTTCATACCTGGGTATTCCGTTCAATCCATCAAAGGCTCCTGGAACAGCTCATCCTTA
TGATAGTGGCCACATTGCAATGACCTACACTGGCCTCTCATGCTTAGTTATTCTTGGAGACGACTTAAGC
CGAGTAAATAAAGAAGCTTGCTTAGCGGGCTTGAGAGCCCTTCAGCTGGAAGATGGGAGTTTTTGTGCAG
TACCTGAAGGCAGTGAAAATGACATGCGATTTGTGTACTGTGCTTCCTGTATTTGCTATATGCTCAACAA
CTGGTCAGGCATGGATATGAAAAAAGCCATCACCTATATTAGAAGGAGTATGTCCTATGACAATGGACTG
GCACAGGGAGCTGGACTTGAATCTCATGGAGGATCAACTTTTTGTGGCATTGCCTCACTATGTCTGATGG
GTAAACTAGAAGAAGTTTTTTCAGAAAAAGAATTGAACAGGATAAAGAGGTGGTGTATAATGAGGCAACA
AAATGGTTATCATGGAAGACCTAATAAGCCTGTAGACACCTGTTATTCTTTTTGGGTGGGAGCAACTCTG
AAGCTTCTAAAAATTTTCCAATACACTAACTTTGAGAAAAATAGAAATTACATCTTATCAACTCAAGATC
GCCTTGTAGGGGGATTTGCCAAGTGGCCAGACAGTCATCCAGATGCTTTGCATGCATACTTTGGGATCTG
TGGCCTGTCACTAATGGAGGAAAGTGGAATTTGTAAAGTTCATCCTGCTCTGAATGTAAGCACACGGACT
TCTGAACGCCTTCTAGATCTCCATCAAAGCTGGAAAACCAAGGACTCTAAACAATGCTCAGAGAATGTAC
ATATCTCCACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222599 representing NM_005023
Red=Cloning site Green=Tags(s)

MVATEDERLAGSGEGERLDFLRDRHVRFFQRCLQVLPERYSSLETSRLTIAFFALSGLDMLDSLDVVNKD
DIIEWIYSLQVLPTEDRSNLNRCGFRGSSYLGIPFNPSKAPGTAHPYDSGHIAMTYTGLSCLVILGDDLS
RVNKEACLAGLRALQLEDGSFCAVPEGSENDMRFVYCASCICYMLNNWSGMDMKKAITYIRRSMSYDNGL
AQGAGLESHGGSTFCGIASLCLMGKLEEVFSEKELNRIKRWCIMRQQNGYHGRPNKPVDTCYSFWVGATL
KLLKIFQYTNFEKNRNYILSTQDRLVGGFAKWPDSHPDALHAYFGICGLSLMEESGICKVHPALNVSTRT
SERLLDLHQSWKTKDSKQCSENVHIST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005023
ORF Size 1131 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005023.2, NP_005014.1
RefSeq Size 1659 bp
RefSeq ORF 1134 bp
Locus ID 5229
UniProt ID P53609
Cytogenetics 5q22.3
Domains prenyltrans
Protein Families Druggable Genome
MW 42.2 kDa
Summary Protein geranylgeranyltransferase type I (GGTase-I) transfers a geranylgeranyl group to the cysteine residue of candidate proteins containing a C-terminal CAAX motif in which 'A' is an aliphatic amino acid and 'X' is leucine (summarized by Zhang et al., 1994 PubMed 8106351). The enzyme is composed of a 48-kD alpha subunit (FNTA; MIM 134635) and a 43-kD beta subunit, encoded by the PGGT1B gene. The FNTA gene encodes the alpha subunit for both GGTase-I and the related enzyme farnesyltransferase.supplied by OMIM, Mar 2010
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"NM_005023" in other vectors (6)
SKU Description Size Price
RC222599L1 Lenti ORF clone of Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B), Myc-DDK-tagged 10 ug
$986.00
RC222599L2 Lenti ORF clone of Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B), mGFP tagged 10 ug
$986.00
RC222599L3 Lenti ORF clone of Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B), Myc-DDK-tagged 10 ug
$986.00
RC222599L4 Lenti ORF clone of Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B), mGFP tagged 10 ug
$986.00
RG222599 PGGT1B (tGFP-tagged) - Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B) 10 ug
$886.00
SC313210 PGGT1B (untagged)-Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B) 10 ug
$457.00

Plasmid Map for RC222599

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222599
Circular map for RC222599

file-alt Citations

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